Antibiogram profile and biofilm forming potential of pseudomonas species isolated from various clinical specimens

C. P. Jamsheera, Ethel Suman

Research output: Contribution to journalArticle

Abstract

Objective: The present study aimed at finding the resistance pattern of Pseudomonas aeruginosa and other Pseudomonas species isolated from various clinical specimens in the laboratory. Methods: A total of 150 isolates of different species of Pseudomonas obtained from various clinical specimens processed at the Microbiology laboratory of Kasturba Medical College, Manipal Academy of Higher Education, were taken for this study. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method and interpreted according to the CLSI guidelines. Biofilm assay was performed by modified O’Toole and Kolter method. The results were analyzed using SPSS 17.0 and Student’s unpaired t-test, Kruskal–Wallis, Mann–Whitney, ANOVA, and Chi-square test. p<0.05 was considered statistically significant. Results: Increased resistance was observed by P. aeruginosa to cefotaxime, cotrimoxazole, levofloxacin, ofloxacin, and ticarcillin clavulanate. There was also a good correlation between antibiotic resistance to aztreonam, netilmicin, and ceftazidime and biofilm production. Results of the present study, therefore, demonstrated the occurrence of resistance to various antipseudomonal agents among the biofilm-producing P. aeruginosa isolates. Conclusion: The present study may help in assessing the seriousness of drug resistance caused by biofilm formation in P. aeruginosa and devise strategies through antibiotic policies to minimize such problems.

Original languageEnglish
Pages (from-to)150-154
Number of pages5
JournalAsian Journal of Pharmaceutical and Clinical Research
Volume11
Issue number10
DOIs
Publication statusPublished - 01-10-2018

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Microbial Sensitivity Tests
Biofilms
Pseudomonas
Pseudomonas aeruginosa
Disk Diffusion Antimicrobial Tests
Netilmicin
Ticarcillin
Anti-Bacterial Agents
Aztreonam
Clavulanic Acid
Levofloxacin
Ofloxacin
Ceftazidime
Cefotaxime
Sulfamethoxazole Drug Combination Trimethoprim
Chi-Square Distribution
Microbial Drug Resistance
Microbiology
Drug Resistance
Analysis of Variance

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

Cite this

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abstract = "Objective: The present study aimed at finding the resistance pattern of Pseudomonas aeruginosa and other Pseudomonas species isolated from various clinical specimens in the laboratory. Methods: A total of 150 isolates of different species of Pseudomonas obtained from various clinical specimens processed at the Microbiology laboratory of Kasturba Medical College, Manipal Academy of Higher Education, were taken for this study. Antibiotic susceptibility testing was performed by Kirby-Bauer disc diffusion method and interpreted according to the CLSI guidelines. Biofilm assay was performed by modified O’Toole and Kolter method. The results were analyzed using SPSS 17.0 and Student’s unpaired t-test, Kruskal–Wallis, Mann–Whitney, ANOVA, and Chi-square test. p<0.05 was considered statistically significant. Results: Increased resistance was observed by P. aeruginosa to cefotaxime, cotrimoxazole, levofloxacin, ofloxacin, and ticarcillin clavulanate. There was also a good correlation between antibiotic resistance to aztreonam, netilmicin, and ceftazidime and biofilm production. Results of the present study, therefore, demonstrated the occurrence of resistance to various antipseudomonal agents among the biofilm-producing P. aeruginosa isolates. Conclusion: The present study may help in assessing the seriousness of drug resistance caused by biofilm formation in P. aeruginosa and devise strategies through antibiotic policies to minimize such problems.",
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