7 Citations (Scopus)

Abstract

Context: Berberis aristata DC (Berberidaceae) is an important medicinal plant with claims of widespread medicinal value in indigenous medicine. It is used by herbal healers to treat oral cancers. Objective: To evaluate the antineoplastic activity of the extracts of Berberis aristata in Ehrlich ascites carcinoma (EAC)-bearing mice with cisplatin as positive control in the advanced stage of tumorigenesis. Materials and methods: Brine shrimp lethality bioassay (BSL) of extracts and effect on the tumor cell viability in vitro were carried out. EAC was induced in Swiss albino mice by injecting 10 6 cell/mL of tumor cell suspension i.p. Antineoplastic activity of the aqueous and ethanol extracts (100 and 6.5mg/kg i.p., respectively) was compared with that of cisplatin (3.5mg/kg i.p.) on the parameters such as percentage increase in weight, median survival time, and hematology. Results: Ethanol extract attenuated percentage increase in weight gain (-6.86±1.50) due to tumor cell proliferation and increased the survival time (19.5 days) when compared to control group (19.10±2.31 and 16 days, respectively). However, the effect was less than that of cisplatin. In vitro cytotoxicity assay as well as BSL test showed the cytotoxic effect of the extracts. Cisplatin and the extracts reversed the tumor-induced alterations in total white blood cell count, differential leukocyte counts, total red blood cell count, and hemoglobin contents. Discussion and conclusion: Of the two extracts, the ethanol extract was observed to be more efficient and the presence of alkaloids and flavonoids may be responsible for the observed anticancer effects.

Original languageEnglish
Pages (from-to)270-277
Number of pages8
JournalPharmaceutical Biology
Volume50
Issue number3
DOIs
Publication statusPublished - 03-2012

Fingerprint

Berberis
Ascites
Cisplatin
Carcinoma
Artemia
Ethanol
Leukocyte Count
Biological Assay
Antineoplastic Agents
Berberidaceae
Neoplasms
Erythrocyte Count
Mouth Neoplasms
Traditional Medicine
Hematology
Medicinal Plants
Alkaloids
Flavonoids
Weight Gain
Cell Survival

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

@article{b0160418c2164b98befd8cfe8c281131,
title = "Anticancer activity of Berberis aristata in Ehrlich ascites carcinoma-bearing mice: A preliminary study",
abstract = "Context: Berberis aristata DC (Berberidaceae) is an important medicinal plant with claims of widespread medicinal value in indigenous medicine. It is used by herbal healers to treat oral cancers. Objective: To evaluate the antineoplastic activity of the extracts of Berberis aristata in Ehrlich ascites carcinoma (EAC)-bearing mice with cisplatin as positive control in the advanced stage of tumorigenesis. Materials and methods: Brine shrimp lethality bioassay (BSL) of extracts and effect on the tumor cell viability in vitro were carried out. EAC was induced in Swiss albino mice by injecting 10 6 cell/mL of tumor cell suspension i.p. Antineoplastic activity of the aqueous and ethanol extracts (100 and 6.5mg/kg i.p., respectively) was compared with that of cisplatin (3.5mg/kg i.p.) on the parameters such as percentage increase in weight, median survival time, and hematology. Results: Ethanol extract attenuated percentage increase in weight gain (-6.86±1.50) due to tumor cell proliferation and increased the survival time (19.5 days) when compared to control group (19.10±2.31 and 16 days, respectively). However, the effect was less than that of cisplatin. In vitro cytotoxicity assay as well as BSL test showed the cytotoxic effect of the extracts. Cisplatin and the extracts reversed the tumor-induced alterations in total white blood cell count, differential leukocyte counts, total red blood cell count, and hemoglobin contents. Discussion and conclusion: Of the two extracts, the ethanol extract was observed to be more efficient and the presence of alkaloids and flavonoids may be responsible for the observed anticancer effects.",
author = "K.S.R. Pai and P. Srilatha and Kumar Suryakant and Setty, {M. Manjunath} and Nayak, {Pawan G.} and Rao, {C. Mallikarjuna} and Baliga, {M. S.}",
year = "2012",
month = "3",
doi = "10.3109/13880209.2011.599035",
language = "English",
volume = "50",
pages = "270--277",
journal = "Pharmaceutical Biology",
issn = "1388-0209",
publisher = "Informa Healthcare",
number = "3",

}

Anticancer activity of Berberis aristata in Ehrlich ascites carcinoma-bearing mice : A preliminary study. / Pai, K.S.R.; Srilatha, P.; Suryakant, Kumar; Setty, M. Manjunath; Nayak, Pawan G.; Rao, C. Mallikarjuna; Baliga, M. S.

In: Pharmaceutical Biology, Vol. 50, No. 3, 03.2012, p. 270-277.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Anticancer activity of Berberis aristata in Ehrlich ascites carcinoma-bearing mice

T2 - A preliminary study

AU - Pai, K.S.R.

AU - Srilatha, P.

AU - Suryakant, Kumar

AU - Setty, M. Manjunath

AU - Nayak, Pawan G.

AU - Rao, C. Mallikarjuna

AU - Baliga, M. S.

PY - 2012/3

Y1 - 2012/3

N2 - Context: Berberis aristata DC (Berberidaceae) is an important medicinal plant with claims of widespread medicinal value in indigenous medicine. It is used by herbal healers to treat oral cancers. Objective: To evaluate the antineoplastic activity of the extracts of Berberis aristata in Ehrlich ascites carcinoma (EAC)-bearing mice with cisplatin as positive control in the advanced stage of tumorigenesis. Materials and methods: Brine shrimp lethality bioassay (BSL) of extracts and effect on the tumor cell viability in vitro were carried out. EAC was induced in Swiss albino mice by injecting 10 6 cell/mL of tumor cell suspension i.p. Antineoplastic activity of the aqueous and ethanol extracts (100 and 6.5mg/kg i.p., respectively) was compared with that of cisplatin (3.5mg/kg i.p.) on the parameters such as percentage increase in weight, median survival time, and hematology. Results: Ethanol extract attenuated percentage increase in weight gain (-6.86±1.50) due to tumor cell proliferation and increased the survival time (19.5 days) when compared to control group (19.10±2.31 and 16 days, respectively). However, the effect was less than that of cisplatin. In vitro cytotoxicity assay as well as BSL test showed the cytotoxic effect of the extracts. Cisplatin and the extracts reversed the tumor-induced alterations in total white blood cell count, differential leukocyte counts, total red blood cell count, and hemoglobin contents. Discussion and conclusion: Of the two extracts, the ethanol extract was observed to be more efficient and the presence of alkaloids and flavonoids may be responsible for the observed anticancer effects.

AB - Context: Berberis aristata DC (Berberidaceae) is an important medicinal plant with claims of widespread medicinal value in indigenous medicine. It is used by herbal healers to treat oral cancers. Objective: To evaluate the antineoplastic activity of the extracts of Berberis aristata in Ehrlich ascites carcinoma (EAC)-bearing mice with cisplatin as positive control in the advanced stage of tumorigenesis. Materials and methods: Brine shrimp lethality bioassay (BSL) of extracts and effect on the tumor cell viability in vitro were carried out. EAC was induced in Swiss albino mice by injecting 10 6 cell/mL of tumor cell suspension i.p. Antineoplastic activity of the aqueous and ethanol extracts (100 and 6.5mg/kg i.p., respectively) was compared with that of cisplatin (3.5mg/kg i.p.) on the parameters such as percentage increase in weight, median survival time, and hematology. Results: Ethanol extract attenuated percentage increase in weight gain (-6.86±1.50) due to tumor cell proliferation and increased the survival time (19.5 days) when compared to control group (19.10±2.31 and 16 days, respectively). However, the effect was less than that of cisplatin. In vitro cytotoxicity assay as well as BSL test showed the cytotoxic effect of the extracts. Cisplatin and the extracts reversed the tumor-induced alterations in total white blood cell count, differential leukocyte counts, total red blood cell count, and hemoglobin contents. Discussion and conclusion: Of the two extracts, the ethanol extract was observed to be more efficient and the presence of alkaloids and flavonoids may be responsible for the observed anticancer effects.

UR - http://www.scopus.com/inward/record.url?scp=84856928110&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856928110&partnerID=8YFLogxK

U2 - 10.3109/13880209.2011.599035

DO - 10.3109/13880209.2011.599035

M3 - Article

C2 - 22085276

AN - SCOPUS:84856928110

VL - 50

SP - 270

EP - 277

JO - Pharmaceutical Biology

JF - Pharmaceutical Biology

SN - 1388-0209

IS - 3

ER -