Antidiabetic activity of benzyl tetra isoquinoline alkaloid berberine in streptozotocin-nicotinamide induced type 2 diabetic rats

I.S.R. Punitha, A. Shirwaikar

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

The antidiabetic potential of berberine was evaluated in a streptozotocin-nicotinamide induced type 2 diabetic rat model. On administration of graded doses of berberine to normal and experimental diabetic rats for 12 days, a significant (p <0.05) reduction was, observed in fasting blood glucose levels. However, serum insulin levels failed to be stimulated on treatment with berberine. Significant changes were observed in serum lipid profiles, thiobarbituric acid reactive substance levels, glycosylated hemoglobin and liver glycogen levels in berberine treated diabetic rats as compared with diabetic control and normal animals. The effect of berberine was also studied for its carbohydrate metabolism and antioxidant status in streptozotocin- nicotinamide induced type 2 diabetic rats. Oral administration of berberine caused a significant increase in both enzymatic and nonenzymatic antioxidants. Studies of the effect of berberine on glycolytic enzymes showed a significant increase in their levels whilst a significant decrease was observed in the levels of the gluconeogenic enzymes in treated diabetic rats. Serum creatinine and urea levels also declined significantly.
Original languageEnglish
Pages (from-to)117-128
Number of pages12
JournalDiabetologia Croatica
Volume34
Issue number4
Publication statusPublished - 2006
Externally publishedYes

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Berberine Alkaloids
Benzylisoquinolines
Berberine
Niacinamide
Streptozocin
Hypoglycemic Agents
Antioxidants
Serum
Liver Glycogen
Thiobarbituric Acid Reactive Substances
Glycosylated Hemoglobin A
Carbohydrate Metabolism
Enzymes
Oral Administration
Blood Glucose
Urea
Fasting
Creatinine
Insulin
Lipids

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@article{2b4a16de012247519b161a685cde502e,
title = "Antidiabetic activity of benzyl tetra isoquinoline alkaloid berberine in streptozotocin-nicotinamide induced type 2 diabetic rats",
abstract = "The antidiabetic potential of berberine was evaluated in a streptozotocin-nicotinamide induced type 2 diabetic rat model. On administration of graded doses of berberine to normal and experimental diabetic rats for 12 days, a significant (p <0.05) reduction was, observed in fasting blood glucose levels. However, serum insulin levels failed to be stimulated on treatment with berberine. Significant changes were observed in serum lipid profiles, thiobarbituric acid reactive substance levels, glycosylated hemoglobin and liver glycogen levels in berberine treated diabetic rats as compared with diabetic control and normal animals. The effect of berberine was also studied for its carbohydrate metabolism and antioxidant status in streptozotocin- nicotinamide induced type 2 diabetic rats. Oral administration of berberine caused a significant increase in both enzymatic and nonenzymatic antioxidants. Studies of the effect of berberine on glycolytic enzymes showed a significant increase in their levels whilst a significant decrease was observed in the levels of the gluconeogenic enzymes in treated diabetic rats. Serum creatinine and urea levels also declined significantly.",
author = "I.S.R. Punitha and A. Shirwaikar",
note = "Cited By :32 Export Date: 10 November 2017 CODEN: DBCRB Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: glycosylated hemoglobin, 9062-63-9; streptozocin, 18883-66-4 References: Stermitz, F.R., Lorenz, P., Tawara, J.N., Zenewica, L.A., Lewis, K., Synergy in a medicinal plant: Antimicrobial action of berberine potentiated by 5′-methoxyhydrocarpin a multidrug pump inhibitor (2001) Proc Natl Acad Sci USA, 97, pp. 1433-1437; Amin, A.H., Subbaiah, T.V., Abbasi, K.M., Berberine sulfate: Antimicrobial activity, bioassay and mode of action (1969) Can J Microbiol, 15, pp. 1067-1076; Mahajan, V.M., Sharma, A., Rattan, A., Antimycotic activity of berberine sulphate: An alkaloid from an Indian medicinal herb (1982) Sabouraudia, 20, pp. 79-81; Subbaiah, T.V., Amin, A.H., Effect of berberine sulfate on Entamoeba histolytica (1967) Nature, 215, pp. 527-528; Gudima, S.O., Memelova, L.V., Borodulin, V.B., Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids (1994) Mol Biol (Mosk), 28, pp. 1308-1314; Singhal, K.C., Anthelmintic activity of berberine hydrochloride in trachoma (1982) Indian J Ophthalmol, 30, pp. 69-75; Mohan, M., Pant, C.R., Angea, S.K., Mahajan, V.M., Berberine in trachoma (1982) Indian J Ophthalmol, 30, pp. 69-75; Sabir, M., Mahajan, V.M., Mohapatra, L.N., Bhide, N.K., Experimental study of the antitrachoma action of berberine (1976) Indian J Med Res, 64, pp. 1160-1167; Taylor, C.T., Baird, A.W., Berberine inhibition of electrogenic ion transport in rat colon (1995) Br J Pharmacol, 116, pp. 2667-2672; Akhter, M.H., Sabir, M., Bhide, N.K., Possible mechanism of antidiarrhoeal effect of berberine (1979) Indian J Med Res, 70, pp. 233-241; Xu, Z., Cao, H.Y., Li, Q., Protective effects of berberine on spontaneous ventricular fibrillation in dogs after myocardial infarction (1989) Chung Kuo Yao Li Hsueh Pao, 10, pp. 320-324; Ckless, K., Schlottfeldt, J., Pasqual, M., Inhibition of in vitro lymphocyte transformation by the isoquinoline alkaloid berberine (1995) J Pharm Pharmacol, 47, pp. 1029-1031; Wu, J.F., Liu, T.P., Effects of berberine in platelet aggregation and plasma levels of TXb2 and 6-keto-PGF1 alpha in rats with reversible middle cerebral artery occlusion (1995) Yao Hsueh Hsueh Pao, 30, pp. 98-102; Chan, M.Y., The effects of berberine on bilirubin excretion in the rat (1977) Gomp Med East West, 5, pp. 161-168; Huang, W., The role and mechanism of berberine on coronary arteries (1990) Chung Hua Hsin Hsueh Kuan Ping Tsa Chih, 18, pp. 231-234; Hua, Z., Wang, X.L., Inhibitory effect of berberine on potassium channels in guinea pig ventricular mycocytes (1994) Acta Pharm Sinica, 29, pp. 576-580; Hua, W.G., Song, J.M., Liao, H., Effect of Huang Lian Su on nerve conduction velocity and hormone level to diabetic neuropathy in rats (2001) Labeled Immunoassays & Clin Med, 8, pp. 212-214; Pan, G.-Y., Huang, Z.-J., Wang, G.-J., The antihyperglycaemic activity of berberine arises from a decrease of glucose absorption (2003) Planta Med, 69, pp. 632-636; Leng, S.H., Lu, E., Xu, L.J., Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion (2004) Acta Pharmacol Sinica, 25, pp. 496-502; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Calcutta: Scientific Book Agency; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , New York: Academic Press; Bonner-Weir, S., Dery, D., John, L.L., Gordon, C.W., Compensatory growth of pancreatic β cells in adult rats after short-term glucose infusion (1989) Diabetes, 38, pp. 49-53; Pellegrino, M., Broca, C., Gross, R., Rove, M., Manteghetti, M., Hillaire-Buys, D., Development of a new model of type 2 diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-229; Nicholas, V., The determination of glycogen in liver and muscle by use of anthrone reagent (1956) Indian J Biol Chem, 220, pp. 583-587; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-358; Brandstrup, N., Kirk, J.E., Bruni, C., Determination of hexokinase in tissues (1957) J Gerontol, 12, pp. 166-171; Deutsch, J., Glucose 6 phosphate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 190. , Deerfield Beach, FL: Verlag Chemie; Vassault, A., Lactate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 118. , Deerfield Beach, FL: Verlag Chemie; Baginsky, E.S., Foa, P.P., Zad, B., Glucose 6-phosphatase (1974) Bergmeyer's Methods in Enzymatic Analysis, 2, pp. 788-792. , New York: Academic Press; Horder, M., Rej, R., Alanine amino transferase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 190. , Deerfield Beach, FL: Verlag Chemie; Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 273. , Deerfield Beach, FL: Verlag Chemie; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Putter, J., Becker, R., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 286. , Deerfield Beach, FL: Verlag Chemie; Kakkar, P., Dos, B., Viswanathan, R.N., A modified spectrophotometric assay of superoxide dismutase (1984) Ind J Biochem Biophys, 21, pp. 130-132; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J Labor Clin Med, 589, pp. 161-168; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods Enzymol, 105, pp. 138-142; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD, eds. New York: Academic Press; Lowry, O.H., Roesborough, M.J., Farr, A.L., Randall, R.J., Protein measurement with Folin- phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Mohamed, A.K., Bierhaus, A., Sciekofer, S., Tritschler, H., Ziegler, H., Nawroth, P.P., The role of oxidative stress and NF (B) activation in late diabetic complications (1999) Biofactors, 10, pp. 175-179; Donnini, D., Zambito, A.M., Perella, G., Ambesi-Impiombat, O., Curcio, F., Glucose may induce cell death through a free radical mediated mechanism (1996) Biochem Biophys Res Commun, 219, pp. 412-417; Baynes, J.W., Thorpe, S.R., Role of oxidative stress in diabetic complications (1999) Diabetes, 48, pp. 1-9; Ozturk, Y., Altan, V.M., Yildizoglu, A., Effect of experimental diabetes and insulin on smooth muscle functions (1996) Pharmacol Rev, 48, pp. 69-112; Swanston-Flat, S.K., Day, C., Bailey, C.J., Flatt, P.R., Traditional plant treatments for diabetes: Studies in normal and streptozotocin diabetic mice (1990) Diabetologia, 33, pp. 462-464; Chase, P.H., Glasgow, A.M., Juvenile diabetes mellitus and serum lipids and lipoprotein levels (1976) Am J Dis Child, 130, p. 1113; Nikkila, E.A., Kekki, M., Plasma transport kinetics in diabetes mellitus (1973) Metabolism, 22, pp. 1-22; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in streptozotocin nicotinamide induced type 2 diabetic rats (2005) J Ethnopharmacol, 97, pp. 369-374; Baquer, N.Z., Gupta, D., Raju, J., Regulation of metabolic pathways in liver and kidney during experimental diabetes: Effects of antidiabetic compounds (1998) Ind J Clin Biochem, 13, pp. 63-80; Ugochukwu, N.H., Babady, N.E., Antihyperglycaemic effect of aqueous and ethanolic extract of Gongronema latifolium levels of glucose and glycogen metabolism in liver of normal and STZ induced diabetic rats (2003) Life Sci, 73, pp. 1924-1938; Burtis, C.A., Ashwood, E.R., Carbohydrate metabolism (1999) Tietz Textbook of Clinical Chemistry, 3rd Edition, pp. 594-655. , Philadelphia: WB. Saunders; Berg, J.M., Tymoczko, J.L., Stryer, L., Glycolysis and gluconeogenesis (2001) Biochemistry, pp. 425-464. , Berg JM, Tymoczko JL, Stryer L, eds. New York: WH. Freeman and Company; Ghosh, S., Suryawanshi, S.A., Effect of Vinca rosea extracts in treatment of alloxan diabetes in male albino rats (2001) Ind J Exp Biol, 39, pp. 748-759; Felig, P., Marliss, E., Ohman, J., Cahill, J.F., Plasma amino acid levels in diabetic ketoacidosis (1970) Diabetes, 19, pp. 727-730; Huang, X., Vaag, A., Hanson, M., Weng, J., Goop, L., Impaired insulin stimulated expression of the glycogen synthase gene in skeletal muscle of type 2 diabetic parents is acquired rather than inherited (2000) J Clin Endocrinol Metab, 85, pp. 1584-1590; Whitton, P.P., Hems, D.A., Glycogen synthesis in perfused liver of STZ diabetic rats (1975) Biochem J, 150, p. 153; Halliwell, B., Gutteridge, J.M.C., (1985) Free Radicals in Biology and Medicine, pp. 1-27. , Oxford: Clarendon Press; McCord, J.M., Keele, B.B., Fridovich, I., An enzyme based theory of obligate anaerobiosis, the physiological functions of superoxide dismutase (1976) Proc Natl Acad Sci USA, 68, pp. 1024-1027; Chance, B., Greenstein, D.S., Roughton, R.J.W., The mechanism of catalase action - Steady state analysis (1952) Arch Biochem Biophys, 37, pp. 301-339; Searle, A.J., Wilson, R., Glutathione peroxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity (1980) Int J Radiat Biol, 37, pp. 213-217; Meister, A., New aspects of glutathione biochemistry and transport selective alterations of glutathione metabolism (1984) Nutr Rev, 42, pp. 397-410; Nicotera, P., Orrenius, S., Role of thiols in protection against biological reactive intermediates (1986) Adv Exp Med Biol, 97, pp. 41-49; Anuradha, C.V., Selvam, R., Effect of oral methionine on tissue lipid peroxidation and antioxidants in alloxan induced diabetic rats (1993) J Nutr Biochem, 4, pp. 212-217; Bruce, A., Freeman, D., James, C., Biology of disease-free radicals and tissue injury (1982) Lab Invest, 47, pp. 412-426; Matcovis, B., Varga, S.I., Szaluo Witsas, H., The effect of diabetes on the activities of the peroxide metabolic enzymes (1982) Horm Metab Res, 14, pp. 77-79; Kinalski, M., Sledziewski, A., Telejko, B., Zarzycki, W., Kinalska, T., Lipid peroxidation and scavenging enzyme activity in streptozotocin induced diabetes (2000) Acta Diabetol, 37, pp. 179-183; Halliwell, B., Gutteridge, J.M.C., The antioxidants of human extracellular fluids (1990) Arch Biochem Biophys, 280, pp. 1-8; Frei, B., England, L., Ames, B.N., Ascorbate is an outstanding antioxidant in human blood plasma (1986) Proc Natl Acad Sci USA, 86, pp. 6377-6381; Inefers, H., Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin e (1988) Eur J Biochem, 174, pp. 353-357; Sajithlal, G.B., Chitra, P., Chandrakasan, G., Effect of curcumin on the advanced glycation and cross linking of collagen in diabetic rats (1998) Biochem Pharmacol, 56, pp. 1607-1614",
year = "2006",
language = "English",
volume = "34",
pages = "117--128",
journal = "Diabetologia Croatica",
issn = "0351-0042",
publisher = "Vuk Vrhovac Institute",
number = "4",

}

Antidiabetic activity of benzyl tetra isoquinoline alkaloid berberine in streptozotocin-nicotinamide induced type 2 diabetic rats. / Punitha, I.S.R.; Shirwaikar, A.

In: Diabetologia Croatica, Vol. 34, No. 4, 2006, p. 117-128.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antidiabetic activity of benzyl tetra isoquinoline alkaloid berberine in streptozotocin-nicotinamide induced type 2 diabetic rats

AU - Punitha, I.S.R.

AU - Shirwaikar, A.

N1 - Cited By :32 Export Date: 10 November 2017 CODEN: DBCRB Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka 576 104, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: glycosylated hemoglobin, 9062-63-9; streptozocin, 18883-66-4 References: Stermitz, F.R., Lorenz, P., Tawara, J.N., Zenewica, L.A., Lewis, K., Synergy in a medicinal plant: Antimicrobial action of berberine potentiated by 5′-methoxyhydrocarpin a multidrug pump inhibitor (2001) Proc Natl Acad Sci USA, 97, pp. 1433-1437; Amin, A.H., Subbaiah, T.V., Abbasi, K.M., Berberine sulfate: Antimicrobial activity, bioassay and mode of action (1969) Can J Microbiol, 15, pp. 1067-1076; Mahajan, V.M., Sharma, A., Rattan, A., Antimycotic activity of berberine sulphate: An alkaloid from an Indian medicinal herb (1982) Sabouraudia, 20, pp. 79-81; Subbaiah, T.V., Amin, A.H., Effect of berberine sulfate on Entamoeba histolytica (1967) Nature, 215, pp. 527-528; Gudima, S.O., Memelova, L.V., Borodulin, V.B., Kinetic analysis of interaction of human immunodeficiency virus reverse transcriptase with alkaloids (1994) Mol Biol (Mosk), 28, pp. 1308-1314; Singhal, K.C., Anthelmintic activity of berberine hydrochloride in trachoma (1982) Indian J Ophthalmol, 30, pp. 69-75; Mohan, M., Pant, C.R., Angea, S.K., Mahajan, V.M., Berberine in trachoma (1982) Indian J Ophthalmol, 30, pp. 69-75; Sabir, M., Mahajan, V.M., Mohapatra, L.N., Bhide, N.K., Experimental study of the antitrachoma action of berberine (1976) Indian J Med Res, 64, pp. 1160-1167; Taylor, C.T., Baird, A.W., Berberine inhibition of electrogenic ion transport in rat colon (1995) Br J Pharmacol, 116, pp. 2667-2672; Akhter, M.H., Sabir, M., Bhide, N.K., Possible mechanism of antidiarrhoeal effect of berberine (1979) Indian J Med Res, 70, pp. 233-241; Xu, Z., Cao, H.Y., Li, Q., Protective effects of berberine on spontaneous ventricular fibrillation in dogs after myocardial infarction (1989) Chung Kuo Yao Li Hsueh Pao, 10, pp. 320-324; Ckless, K., Schlottfeldt, J., Pasqual, M., Inhibition of in vitro lymphocyte transformation by the isoquinoline alkaloid berberine (1995) J Pharm Pharmacol, 47, pp. 1029-1031; Wu, J.F., Liu, T.P., Effects of berberine in platelet aggregation and plasma levels of TXb2 and 6-keto-PGF1 alpha in rats with reversible middle cerebral artery occlusion (1995) Yao Hsueh Hsueh Pao, 30, pp. 98-102; Chan, M.Y., The effects of berberine on bilirubin excretion in the rat (1977) Gomp Med East West, 5, pp. 161-168; Huang, W., The role and mechanism of berberine on coronary arteries (1990) Chung Hua Hsin Hsueh Kuan Ping Tsa Chih, 18, pp. 231-234; Hua, Z., Wang, X.L., Inhibitory effect of berberine on potassium channels in guinea pig ventricular mycocytes (1994) Acta Pharm Sinica, 29, pp. 576-580; Hua, W.G., Song, J.M., Liao, H., Effect of Huang Lian Su on nerve conduction velocity and hormone level to diabetic neuropathy in rats (2001) Labeled Immunoassays & Clin Med, 8, pp. 212-214; Pan, G.-Y., Huang, Z.-J., Wang, G.-J., The antihyperglycaemic activity of berberine arises from a decrease of glucose absorption (2003) Planta Med, 69, pp. 632-636; Leng, S.H., Lu, E., Xu, L.J., Therapeutic effects of berberine in impaired glucose tolerance rats and its influence on insulin secretion (2004) Acta Pharmacol Sinica, 25, pp. 496-502; Ghosh, M.N., Toxicity studies (1984) Fundamentals of Experimental Pharmacology, pp. 153-158. , Calcutta: Scientific Book Agency; Turner, M.A., (1965) Screening Methods in Pharmacology, p. 26. , New York: Academic Press; Bonner-Weir, S., Dery, D., John, L.L., Gordon, C.W., Compensatory growth of pancreatic β cells in adult rats after short-term glucose infusion (1989) Diabetes, 38, pp. 49-53; Pellegrino, M., Broca, C., Gross, R., Rove, M., Manteghetti, M., Hillaire-Buys, D., Development of a new model of type 2 diabetes in adult rats administered with streptozotocin and nicotinamide (1998) Diabetes, 47, pp. 224-229; Nicholas, V., The determination of glycogen in liver and muscle by use of anthrone reagent (1956) Indian J Biol Chem, 220, pp. 583-587; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-358; Brandstrup, N., Kirk, J.E., Bruni, C., Determination of hexokinase in tissues (1957) J Gerontol, 12, pp. 166-171; Deutsch, J., Glucose 6 phosphate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 190. , Deerfield Beach, FL: Verlag Chemie; Vassault, A., Lactate dehydrogenase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 118. , Deerfield Beach, FL: Verlag Chemie; Baginsky, E.S., Foa, P.P., Zad, B., Glucose 6-phosphatase (1974) Bergmeyer's Methods in Enzymatic Analysis, 2, pp. 788-792. , New York: Academic Press; Horder, M., Rej, R., Alanine amino transferase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 190. , Deerfield Beach, FL: Verlag Chemie; Rotruck, J.T., Pope, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 273. , Deerfield Beach, FL: Verlag Chemie; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Putter, J., Becker, R., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 286. , Deerfield Beach, FL: Verlag Chemie; Kakkar, P., Dos, B., Viswanathan, R.N., A modified spectrophotometric assay of superoxide dismutase (1984) Ind J Biochem Biophys, 21, pp. 130-132; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J Labor Clin Med, 589, pp. 161-168; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods Enzymol, 105, pp. 138-142; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD, eds. New York: Academic Press; Lowry, O.H., Roesborough, M.J., Farr, A.L., Randall, R.J., Protein measurement with Folin- phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Mohamed, A.K., Bierhaus, A., Sciekofer, S., Tritschler, H., Ziegler, H., Nawroth, P.P., The role of oxidative stress and NF (B) activation in late diabetic complications (1999) Biofactors, 10, pp. 175-179; Donnini, D., Zambito, A.M., Perella, G., Ambesi-Impiombat, O., Curcio, F., Glucose may induce cell death through a free radical mediated mechanism (1996) Biochem Biophys Res Commun, 219, pp. 412-417; Baynes, J.W., Thorpe, S.R., Role of oxidative stress in diabetic complications (1999) Diabetes, 48, pp. 1-9; Ozturk, Y., Altan, V.M., Yildizoglu, A., Effect of experimental diabetes and insulin on smooth muscle functions (1996) Pharmacol Rev, 48, pp. 69-112; Swanston-Flat, S.K., Day, C., Bailey, C.J., Flatt, P.R., Traditional plant treatments for diabetes: Studies in normal and streptozotocin diabetic mice (1990) Diabetologia, 33, pp. 462-464; Chase, P.H., Glasgow, A.M., Juvenile diabetes mellitus and serum lipids and lipoprotein levels (1976) Am J Dis Child, 130, p. 1113; Nikkila, E.A., Kekki, M., Plasma transport kinetics in diabetes mellitus (1973) Metabolism, 22, pp. 1-22; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in streptozotocin nicotinamide induced type 2 diabetic rats (2005) J Ethnopharmacol, 97, pp. 369-374; Baquer, N.Z., Gupta, D., Raju, J., Regulation of metabolic pathways in liver and kidney during experimental diabetes: Effects of antidiabetic compounds (1998) Ind J Clin Biochem, 13, pp. 63-80; Ugochukwu, N.H., Babady, N.E., Antihyperglycaemic effect of aqueous and ethanolic extract of Gongronema latifolium levels of glucose and glycogen metabolism in liver of normal and STZ induced diabetic rats (2003) Life Sci, 73, pp. 1924-1938; Burtis, C.A., Ashwood, E.R., Carbohydrate metabolism (1999) Tietz Textbook of Clinical Chemistry, 3rd Edition, pp. 594-655. , Philadelphia: WB. Saunders; Berg, J.M., Tymoczko, J.L., Stryer, L., Glycolysis and gluconeogenesis (2001) Biochemistry, pp. 425-464. , Berg JM, Tymoczko JL, Stryer L, eds. New York: WH. 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PY - 2006

Y1 - 2006

N2 - The antidiabetic potential of berberine was evaluated in a streptozotocin-nicotinamide induced type 2 diabetic rat model. On administration of graded doses of berberine to normal and experimental diabetic rats for 12 days, a significant (p <0.05) reduction was, observed in fasting blood glucose levels. However, serum insulin levels failed to be stimulated on treatment with berberine. Significant changes were observed in serum lipid profiles, thiobarbituric acid reactive substance levels, glycosylated hemoglobin and liver glycogen levels in berberine treated diabetic rats as compared with diabetic control and normal animals. The effect of berberine was also studied for its carbohydrate metabolism and antioxidant status in streptozotocin- nicotinamide induced type 2 diabetic rats. Oral administration of berberine caused a significant increase in both enzymatic and nonenzymatic antioxidants. Studies of the effect of berberine on glycolytic enzymes showed a significant increase in their levels whilst a significant decrease was observed in the levels of the gluconeogenic enzymes in treated diabetic rats. Serum creatinine and urea levels also declined significantly.

AB - The antidiabetic potential of berberine was evaluated in a streptozotocin-nicotinamide induced type 2 diabetic rat model. On administration of graded doses of berberine to normal and experimental diabetic rats for 12 days, a significant (p <0.05) reduction was, observed in fasting blood glucose levels. However, serum insulin levels failed to be stimulated on treatment with berberine. Significant changes were observed in serum lipid profiles, thiobarbituric acid reactive substance levels, glycosylated hemoglobin and liver glycogen levels in berberine treated diabetic rats as compared with diabetic control and normal animals. The effect of berberine was also studied for its carbohydrate metabolism and antioxidant status in streptozotocin- nicotinamide induced type 2 diabetic rats. Oral administration of berberine caused a significant increase in both enzymatic and nonenzymatic antioxidants. Studies of the effect of berberine on glycolytic enzymes showed a significant increase in their levels whilst a significant decrease was observed in the levels of the gluconeogenic enzymes in treated diabetic rats. Serum creatinine and urea levels also declined significantly.

M3 - Article

VL - 34

SP - 117

EP - 128

JO - Diabetologia Croatica

JF - Diabetologia Croatica

SN - 0351-0042

IS - 4

ER -