Antidiabetic and hypolipidemic effect of salacia oblonga in streptozotocin induced diabetic rats

Bhagyajyothi M. Bhat, C. V. Raghuveer, Vivian D'Souza, Poornima A. Manjrekar

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objectives: The present study was conducted to evaluate the effect of a standardized hydroalcoholic root extract of Salacia oblonga (SOE) on the Random Blood Glucose (RBG) levels, serum insulin, glycated haemoglobin (HbA1c) and the serum lipid profile in long standing, experimentally induced Diabetes Mellitus (DM) with glibenclamide (Glb) as the standard. Materials and Methods: Streptozotocin (STZ) induced, dia-betic, Wistar rats of either sex were treated with two oral doses of SOE, 100 and 50mg/kg body wt /day, for a period of 16 weeks. The RBG was estimated at day-1 and at the end of the 16 weeks by using a glucometer. The fasting serum insulin was determined by an ELISA technique. The plasma HbA1c was evaluated by a Turbidimetric Inhibition Immunoassay (TINIA) and the lipid profile was estimated enzymatically. Results and Analysis: A 45% decrease in the RBG was seen after the treatment with the higher dose of SOE, whereas a 44% decrease was observed with the lower dose as compared to the diabetic control. Serum insulin was significantly increased (P<0.05) in all the treated groups as compared to the diabetic control. Plasma HbA1c was significantly decreased (P<0.05). The serum Triacyl Glycerol (TG) levels were significantly decreased (P<0.05) in the treated rats as compared to the diabetic control. A significant increase in HDL-cholesterol (P<0.05) in the diabetic rats as a result of the 100mg/kg SOE treatment was a remarkable finding. Conclusion: SOE improves the glycaemic parameters in diabetic rats after a prolonged treatment. The serum TG levels were normalized on treatment. A higher dose of the extract could not alter the parameters significantly, except for HDL-C.

Original languageEnglish
Pages (from-to)1685-1687
Number of pages3
JournalJournal of Clinical and Diagnostic Research
Volume6
Issue number10
DOIs
Publication statusPublished - 15-12-2012

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Salacia
Streptozocin
Hypoglycemic Agents
Rats
Blood Glucose
Serum
Glycerol
Insulin
Lipids
Plasmas
Glyburide
Glycosylated Hemoglobin A
Medical problems
HDL Cholesterol
Therapeutics
Immunoassay
Wistar Rats
Fasting
Diabetes Mellitus
Enzyme-Linked Immunosorbent Assay

All Science Journal Classification (ASJC) codes

  • Clinical Biochemistry

Cite this

@article{b4832da642004d45818b050b69d6cacc,
title = "Antidiabetic and hypolipidemic effect of salacia oblonga in streptozotocin induced diabetic rats",
abstract = "Objectives: The present study was conducted to evaluate the effect of a standardized hydroalcoholic root extract of Salacia oblonga (SOE) on the Random Blood Glucose (RBG) levels, serum insulin, glycated haemoglobin (HbA1c) and the serum lipid profile in long standing, experimentally induced Diabetes Mellitus (DM) with glibenclamide (Glb) as the standard. Materials and Methods: Streptozotocin (STZ) induced, dia-betic, Wistar rats of either sex were treated with two oral doses of SOE, 100 and 50mg/kg body wt /day, for a period of 16 weeks. The RBG was estimated at day-1 and at the end of the 16 weeks by using a glucometer. The fasting serum insulin was determined by an ELISA technique. The plasma HbA1c was evaluated by a Turbidimetric Inhibition Immunoassay (TINIA) and the lipid profile was estimated enzymatically. Results and Analysis: A 45{\%} decrease in the RBG was seen after the treatment with the higher dose of SOE, whereas a 44{\%} decrease was observed with the lower dose as compared to the diabetic control. Serum insulin was significantly increased (P<0.05) in all the treated groups as compared to the diabetic control. Plasma HbA1c was significantly decreased (P<0.05). The serum Triacyl Glycerol (TG) levels were significantly decreased (P<0.05) in the treated rats as compared to the diabetic control. A significant increase in HDL-cholesterol (P<0.05) in the diabetic rats as a result of the 100mg/kg SOE treatment was a remarkable finding. Conclusion: SOE improves the glycaemic parameters in diabetic rats after a prolonged treatment. The serum TG levels were normalized on treatment. A higher dose of the extract could not alter the parameters significantly, except for HDL-C.",
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Antidiabetic and hypolipidemic effect of salacia oblonga in streptozotocin induced diabetic rats. / Bhat, Bhagyajyothi M.; Raghuveer, C. V.; D'Souza, Vivian; Manjrekar, Poornima A.

In: Journal of Clinical and Diagnostic Research, Vol. 6, No. 10, 15.12.2012, p. 1685-1687.

Research output: Contribution to journalArticle

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T1 - Antidiabetic and hypolipidemic effect of salacia oblonga in streptozotocin induced diabetic rats

AU - Bhat, Bhagyajyothi M.

AU - Raghuveer, C. V.

AU - D'Souza, Vivian

AU - Manjrekar, Poornima A.

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Y1 - 2012/12/15

N2 - Objectives: The present study was conducted to evaluate the effect of a standardized hydroalcoholic root extract of Salacia oblonga (SOE) on the Random Blood Glucose (RBG) levels, serum insulin, glycated haemoglobin (HbA1c) and the serum lipid profile in long standing, experimentally induced Diabetes Mellitus (DM) with glibenclamide (Glb) as the standard. Materials and Methods: Streptozotocin (STZ) induced, dia-betic, Wistar rats of either sex were treated with two oral doses of SOE, 100 and 50mg/kg body wt /day, for a period of 16 weeks. The RBG was estimated at day-1 and at the end of the 16 weeks by using a glucometer. The fasting serum insulin was determined by an ELISA technique. The plasma HbA1c was evaluated by a Turbidimetric Inhibition Immunoassay (TINIA) and the lipid profile was estimated enzymatically. Results and Analysis: A 45% decrease in the RBG was seen after the treatment with the higher dose of SOE, whereas a 44% decrease was observed with the lower dose as compared to the diabetic control. Serum insulin was significantly increased (P<0.05) in all the treated groups as compared to the diabetic control. Plasma HbA1c was significantly decreased (P<0.05). The serum Triacyl Glycerol (TG) levels were significantly decreased (P<0.05) in the treated rats as compared to the diabetic control. A significant increase in HDL-cholesterol (P<0.05) in the diabetic rats as a result of the 100mg/kg SOE treatment was a remarkable finding. Conclusion: SOE improves the glycaemic parameters in diabetic rats after a prolonged treatment. The serum TG levels were normalized on treatment. A higher dose of the extract could not alter the parameters significantly, except for HDL-C.

AB - Objectives: The present study was conducted to evaluate the effect of a standardized hydroalcoholic root extract of Salacia oblonga (SOE) on the Random Blood Glucose (RBG) levels, serum insulin, glycated haemoglobin (HbA1c) and the serum lipid profile in long standing, experimentally induced Diabetes Mellitus (DM) with glibenclamide (Glb) as the standard. Materials and Methods: Streptozotocin (STZ) induced, dia-betic, Wistar rats of either sex were treated with two oral doses of SOE, 100 and 50mg/kg body wt /day, for a period of 16 weeks. The RBG was estimated at day-1 and at the end of the 16 weeks by using a glucometer. The fasting serum insulin was determined by an ELISA technique. The plasma HbA1c was evaluated by a Turbidimetric Inhibition Immunoassay (TINIA) and the lipid profile was estimated enzymatically. Results and Analysis: A 45% decrease in the RBG was seen after the treatment with the higher dose of SOE, whereas a 44% decrease was observed with the lower dose as compared to the diabetic control. Serum insulin was significantly increased (P<0.05) in all the treated groups as compared to the diabetic control. Plasma HbA1c was significantly decreased (P<0.05). The serum Triacyl Glycerol (TG) levels were significantly decreased (P<0.05) in the treated rats as compared to the diabetic control. A significant increase in HDL-cholesterol (P<0.05) in the diabetic rats as a result of the 100mg/kg SOE treatment was a remarkable finding. Conclusion: SOE improves the glycaemic parameters in diabetic rats after a prolonged treatment. The serum TG levels were normalized on treatment. A higher dose of the extract could not alter the parameters significantly, except for HDL-C.

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