Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study

V.P. Veerapur, K.R. Prabhakar, B.S. Thippeswamy, P. Bansal, K.K. Srinivasan, M.K. Unnikrishnan

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

To study the effect and mode of action of water extract (DVW) and polar fraction of ethanol extract (DVE-4) of D. viscosa in high-fructose diet induced insulin resistance in male Wistar rats. D. viscosa's effects were evaluated on a battery of targets involved in glucose homeostasis (in vitro studies). Rats were rendered insulin resistant by feeding 66% (w/w) fructose and 1.1% (v/w) coconut oil mixed with normal pellet diet (NPD) for six weeks. DVW and DVE4 at different doses were administered simultaneously. At the end of the study, blood glucose, oral glucose tolerance test, lipid profile and insulin were estimated and homeostatic model assessment (HOMA) levels were calculated. In addition, enzymatic and nonenzymatic liver antioxidant levels were also estimated. Quantification of biomarker quercetin was done using HPLC. Fructose diet with DVW, DVE-4 significantly reduced blood glucose, serum insulin, HOMA, lipid profiles and significantly improved glucose tolerance and HDL-c levels. In addition, these extract and fraction also decreased oxidative stress by improving endogenous antioxidants. In different bioassays, DVW and DVE-4 inhibited protein tyrosine phosphatase-1B with IC50 65.8 and 54.9 μg/ml respectively and showed partial inhibition of dipeptidyl peptidase-IV. Moreover, DVW and DVE-4, at 10 μg/ml showed 60 and 54.2% binding to peroxisome proliferator-activated receptor-γ. Further, 2.1% (w/w) of quercetin was quantified in bioactive-DVE-4 using HPLC method. The results provide pharmacological evidence of D. viscosa in treatment of prediabetic conditions and these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.
Original languageEnglish
Pages (from-to)800-810
Number of pages11
JournalIndian Journal of Experimental Biology
Volume48
Issue number8
Publication statusPublished - 2010

Fingerprint

Fructose
Hypoglycemic Agents
Quercetin
Insulin
Diet
Blood Glucose
Antioxidants
Non-Receptor Type 1 Protein Tyrosine Phosphatase
High Pressure Liquid Chromatography
Dipeptidyl Peptidase 4
Lipids
Glucose
Peroxisome Proliferator-Activated Receptors
Glucose Tolerance Test
Biological Assay
Inhibitory Concentration 50
Insulin Resistance
Wistar Rats
Diabetes Mellitus
Oxidative Stress

Cite this

Veerapur, V. P., Prabhakar, K. R., Thippeswamy, B. S., Bansal, P., Srinivasan, K. K., & Unnikrishnan, M. K. (2010). Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study. Indian Journal of Experimental Biology, 48(8), 800-810.
Veerapur, V.P. ; Prabhakar, K.R. ; Thippeswamy, B.S. ; Bansal, P. ; Srinivasan, K.K. ; Unnikrishnan, M.K. / Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study. In: Indian Journal of Experimental Biology. 2010 ; Vol. 48, No. 8. pp. 800-810.
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abstract = "To study the effect and mode of action of water extract (DVW) and polar fraction of ethanol extract (DVE-4) of D. viscosa in high-fructose diet induced insulin resistance in male Wistar rats. D. viscosa's effects were evaluated on a battery of targets involved in glucose homeostasis (in vitro studies). Rats were rendered insulin resistant by feeding 66{\%} (w/w) fructose and 1.1{\%} (v/w) coconut oil mixed with normal pellet diet (NPD) for six weeks. DVW and DVE4 at different doses were administered simultaneously. At the end of the study, blood glucose, oral glucose tolerance test, lipid profile and insulin were estimated and homeostatic model assessment (HOMA) levels were calculated. In addition, enzymatic and nonenzymatic liver antioxidant levels were also estimated. Quantification of biomarker quercetin was done using HPLC. Fructose diet with DVW, DVE-4 significantly reduced blood glucose, serum insulin, HOMA, lipid profiles and significantly improved glucose tolerance and HDL-c levels. In addition, these extract and fraction also decreased oxidative stress by improving endogenous antioxidants. In different bioassays, DVW and DVE-4 inhibited protein tyrosine phosphatase-1B with IC50 65.8 and 54.9 μg/ml respectively and showed partial inhibition of dipeptidyl peptidase-IV. Moreover, DVW and DVE-4, at 10 μg/ml showed 60 and 54.2{\%} binding to peroxisome proliferator-activated receptor-γ. Further, 2.1{\%} (w/w) of quercetin was quantified in bioactive-DVE-4 using HPLC method. The results provide pharmacological evidence of D. viscosa in treatment of prediabetic conditions and these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.",
author = "V.P. Veerapur and K.R. Prabhakar and B.S. Thippeswamy and P. Bansal and K.K. Srinivasan and M.K. Unnikrishnan",
note = "Cited By :25 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Veerapur, V. P.; Sree Siddaganga College of Pharmacy, Tumkur 572 102, India; email: veeresh36@gmail.com Chemicals/CAS: dipeptidyl peptidase IV, 54249-88-6; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; insulin, 9004-10-8; quercetin, 117-39-5; Antioxidants; Blood Glucose; Dipeptidyl Peptidase 4, 3.4.14.5; Fructose, 30237-26-4; Hypoglycemic Agents; Insulin, 11061-68-0; PPAR gamma; Plant Extracts; Protein Tyrosine Phosphatase, Non-Receptor Type 1, 3.1.3.48; Quercetin, 117-39-5 References: Keller, K.B., Lemberg, L., Obesity and the metabolic syndrome (2003) Am J Crit Care, 12, p. 167; Zimmet, P., Albert, K.G., Shaw, J., Global and societal implications of the diabetes epidemic (2001) Nature, 414, p. 782; Kasim-Karakas, S.E., Vriend, H., Almario, R., Chow, L.C., Goodman, M.N., Effects of dietary carbohydrates on glucose and lipid metabolism in golden Syrian hamsters (1996) J Lab Clin Med, 128, p. 208; Perry, L.M., Metzger, J., (1980) Medicinal plants of East Southeast Asia, p. 375. , (MIT Press, Cambridge, London); Kirtikar, K.R., Basu, B.D., (1993) Indian medicinal plants, 1, p. 641. , 2nd ed., (International Book Publisher, Dehradun, India); Getie, M., Gebre-Mariam, T., Rietz, R., Hohne, C., Huschka, C., Schmidtke, M., Abate, A., Neubert, R.H., Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants (2003) Dodonaea Viscosa, Rumex Nervosus and Rumex Abyssinicus, Fitoterapia, 74 (1-2), p. 139; Veerapur, V.P., Badiger, A.M., Joshi, S.D., Nayak, V.P., Shastry, C.S., Antiulcerogenic activity of various extracts of Dodonaea viscosa (L) Jacq. leaves (2004) Indian J Pharm Sci, 66 (4), p. 407; Joshi, S.D., Badiger, A.M., Ashok, K., Veerapur, V.P., Shastry, C.S., Wound healing activity of Dodonaea viscosa leaves (2003) Indian Drugs, 40 (9), p. 549; Rajas, A., Cruz, S., Ponce-Monter, H., Mata, R., Smooth muscle relaxing compounds from Dodonaea viscosa (1996) Planta Med, 62, p. 154; Aswal, B.S., Bhakuni, D.S., Goel, A.K., Kar, K., Mehrotra, B.N., Screening of Indian Plants for Biological Activity-Part XI (1984) Indian J Exp Biol, 22, p. 487; Veerapur, V.P., (2007) Activity Guided Phytopharmacological Analysis of Selected Indian Medicinal Plants with Special Reference to Diabetes Mellitus, , Ph.D. thesis, Manipal University, Manipal, India; Veerapur, V.P., Prabhakar, K.R., Machendar Reddy, K., Srinivasan, K.K., Unnikrishnan, M.K., Antidiabetic effect of Dodonaea viscosa aerial parts in high fat diet and low-dose streptozotocin-induced type 2 diabetic rats: Mechanistic approach (2010) Pharmaceutical Biol, , (in press); Hevener, A., Reichart, D., Janez, A., Olefsky, J., Female rats do not exhibit free fatty acid-induced insulin resistance (2002) Diabetes, 51, p. 1907; Galipeau, D., Verma, S., McNeill, J.H., Female rats are protected against fructose-induced changes in metabolism and blood pressure (2002) Am J Physiol Heart Circ Physiol, 283, pp. H2478; Rajasekar, P., Kaviarasan, S., Anuradha, C.V., L-Carnitine administration prevents oxidative stress in high fructose-fed insulin resistant rats (2005) Diabetologica Croatica, 34 (1), p. 21; Pickavance, L.C., Tadayyon, M., Widdowson, P.S., Buckingham, R.E., Wilding, J.P.H., Therapeutic index for rosiglitazone in dietary obese rats: Separation of efficacy and haemodilution (1999) Brit J Pharmacol, 128, p. 1570; Friedewald, W.T., Levy, R.I., Fredrickson, D.S., Estimation of low-density lipoprotein cholesterol in plasma, without use of the preparative centrifuge (1972) Clin Chem, 18, p. 499; Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, p. 265; Prabhakar, K.R., Veerapur, V.P., Parihar, K.V., Priyadarsini, K.I., Rao, B.S., Unnikrishnan, M.K., Evaluation and optimization of radioprotective activity of Coronopus didymus Linn. in gamma-irradiated mice (2006) Int J Rad Biol, 82, p. 525; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochimica Biophysica Acta, 1035, p. 353; Zang, Z.Y., Lee, S.Y., PTP-1B inhibitors as potential therapeutics in treatment of type 2 diabetes (2003) Exp Opin Investig Drugs, 12 (2), p. 223; Ji-Hu, Z., Chung, T.D.Y., Oldenburg, K.R., A simple statistical parameter for use in evaluation and validation of high throughput screening assays (1999) J Biomol Screen, 4, p. 67; Kojima, K., Hama, T., Kato, T., Nagastu, T., Rapid chromatogrphic purification of DPP-IV in human submaxillary gland (1980) J. Chromatography, 189, p. 233; Liang-Yi, W., Chi-Chang, J., Lucy Sun, H., Yung-Pei, H., Pei-Hsuan, H., Low-Tone, H., Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model (2004) Eur J Nutr, 43, p. 116; Kannappan, S., Jayaraman, T., Rajasekar, P., Ravichandran, M.K., Anuradha, C.V., Cinnamon bark extract improves glucose metabolism and lipid profile in the fructose-fed rat (2006) Singapore Med J, 47 (10), p. 858; Shimizu, S., Ugi, S., Maegawa, H., Egawa, K., Nishio, Y., Yoshizaki, T., Shi, K., Nemoto, K., Protein-tyrosine phosphatase I B as new activator for hepatic lipogenesis via sterol regulatory element-binding protein-1 gene expression (2003) J Biol Chem, 278, p. 43095; Faure, P., Rossinoc, E., Wiernsperger, N., Richard, M.J., Favier, A., Halimi, S., Vitamin E improves the free radical defense system potential and insulin sensitivity of rats fed high fructose diets (1997) J Nutr, 127, p. 103; Mahesh, T., Menon Venugopal, P., Quercetin alleviates oxidative stress in streptozotocin-induced diabetic rats (2004) Phytother Res, 18, p. 123; Vessal, M., Hemmati, M., Vasei, M., Antidiabetic effects of quercetin in streptozocin-induced diabetic rats (2003) Comp Biochem Physiol C Toxicol Pharmacol, 135 C (3), p. 357",
year = "2010",
language = "English",
volume = "48",
pages = "800--810",
journal = "Journal of scientific & industrial research. C. Biological sciences",
issn = "0019-5189",
publisher = "National Institute of Science Communication",
number = "8",

}

Veerapur, VP, Prabhakar, KR, Thippeswamy, BS, Bansal, P, Srinivasan, KK & Unnikrishnan, MK 2010, 'Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study', Indian Journal of Experimental Biology, vol. 48, no. 8, pp. 800-810.

Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study. / Veerapur, V.P.; Prabhakar, K.R.; Thippeswamy, B.S.; Bansal, P.; Srinivasan, K.K.; Unnikrishnan, M.K.

In: Indian Journal of Experimental Biology, Vol. 48, No. 8, 2010, p. 800-810.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antidiabetic effect of Dodonaea viscosa (L). Lacq. aerial parts in high fructose-fed insulin resistant rats: A mechanism based study

AU - Veerapur, V.P.

AU - Prabhakar, K.R.

AU - Thippeswamy, B.S.

AU - Bansal, P.

AU - Srinivasan, K.K.

AU - Unnikrishnan, M.K.

N1 - Cited By :25 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Veerapur, V. P.; Sree Siddaganga College of Pharmacy, Tumkur 572 102, India; email: veeresh36@gmail.com Chemicals/CAS: dipeptidyl peptidase IV, 54249-88-6; fructose, 30237-26-4, 57-48-7, 7660-25-5, 77907-44-9; insulin, 9004-10-8; quercetin, 117-39-5; Antioxidants; Blood Glucose; Dipeptidyl Peptidase 4, 3.4.14.5; Fructose, 30237-26-4; Hypoglycemic Agents; Insulin, 11061-68-0; PPAR gamma; Plant Extracts; Protein Tyrosine Phosphatase, Non-Receptor Type 1, 3.1.3.48; Quercetin, 117-39-5 References: Keller, K.B., Lemberg, L., Obesity and the metabolic syndrome (2003) Am J Crit Care, 12, p. 167; Zimmet, P., Albert, K.G., Shaw, J., Global and societal implications of the diabetes epidemic (2001) Nature, 414, p. 782; Kasim-Karakas, S.E., Vriend, H., Almario, R., Chow, L.C., Goodman, M.N., Effects of dietary carbohydrates on glucose and lipid metabolism in golden Syrian hamsters (1996) J Lab Clin Med, 128, p. 208; Perry, L.M., Metzger, J., (1980) Medicinal plants of East Southeast Asia, p. 375. , (MIT Press, Cambridge, London); Kirtikar, K.R., Basu, B.D., (1993) Indian medicinal plants, 1, p. 641. , 2nd ed., (International Book Publisher, Dehradun, India); Getie, M., Gebre-Mariam, T., Rietz, R., Hohne, C., Huschka, C., Schmidtke, M., Abate, A., Neubert, R.H., Evaluation of the anti-microbial and anti-inflammatory activities of the medicinal plants (2003) Dodonaea Viscosa, Rumex Nervosus and Rumex Abyssinicus, Fitoterapia, 74 (1-2), p. 139; Veerapur, V.P., Badiger, A.M., Joshi, S.D., Nayak, V.P., Shastry, C.S., Antiulcerogenic activity of various extracts of Dodonaea viscosa (L) Jacq. leaves (2004) Indian J Pharm Sci, 66 (4), p. 407; Joshi, S.D., Badiger, A.M., Ashok, K., Veerapur, V.P., Shastry, C.S., Wound healing activity of Dodonaea viscosa leaves (2003) Indian Drugs, 40 (9), p. 549; Rajas, A., Cruz, S., Ponce-Monter, H., Mata, R., Smooth muscle relaxing compounds from Dodonaea viscosa (1996) Planta Med, 62, p. 154; Aswal, B.S., Bhakuni, D.S., Goel, A.K., Kar, K., Mehrotra, B.N., Screening of Indian Plants for Biological Activity-Part XI (1984) Indian J Exp Biol, 22, p. 487; Veerapur, V.P., (2007) Activity Guided Phytopharmacological Analysis of Selected Indian Medicinal Plants with Special Reference to Diabetes Mellitus, , Ph.D. thesis, Manipal University, Manipal, India; Veerapur, V.P., Prabhakar, K.R., Machendar Reddy, K., Srinivasan, K.K., Unnikrishnan, M.K., Antidiabetic effect of Dodonaea viscosa aerial parts in high fat diet and low-dose streptozotocin-induced type 2 diabetic rats: Mechanistic approach (2010) Pharmaceutical Biol, , (in press); Hevener, A., Reichart, D., Janez, A., Olefsky, J., Female rats do not exhibit free fatty acid-induced insulin resistance (2002) Diabetes, 51, p. 1907; Galipeau, D., Verma, S., McNeill, J.H., Female rats are protected against fructose-induced changes in metabolism and blood pressure (2002) Am J Physiol Heart Circ Physiol, 283, pp. H2478; Rajasekar, P., Kaviarasan, S., Anuradha, C.V., L-Carnitine administration prevents oxidative stress in high fructose-fed insulin resistant rats (2005) Diabetologica Croatica, 34 (1), p. 21; Pickavance, L.C., Tadayyon, M., Widdowson, P.S., Buckingham, R.E., Wilding, J.P.H., Therapeutic index for rosiglitazone in dietary obese rats: Separation of efficacy and haemodilution (1999) Brit J Pharmacol, 128, p. 1570; Friedewald, W.T., Levy, R.I., Fredrickson, D.S., Estimation of low-density lipoprotein cholesterol in plasma, without use of the preparative centrifuge (1972) Clin Chem, 18, p. 499; Lowry, O.H., Rosenbrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J Biol Chem, 193, p. 265; Prabhakar, K.R., Veerapur, V.P., Parihar, K.V., Priyadarsini, K.I., Rao, B.S., Unnikrishnan, M.K., Evaluation and optimization of radioprotective activity of Coronopus didymus Linn. in gamma-irradiated mice (2006) Int J Rad Biol, 82, p. 525; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochimica Biophysica Acta, 1035, p. 353; Zang, Z.Y., Lee, S.Y., PTP-1B inhibitors as potential therapeutics in treatment of type 2 diabetes (2003) Exp Opin Investig Drugs, 12 (2), p. 223; Ji-Hu, Z., Chung, T.D.Y., Oldenburg, K.R., A simple statistical parameter for use in evaluation and validation of high throughput screening assays (1999) J Biomol Screen, 4, p. 67; Kojima, K., Hama, T., Kato, T., Nagastu, T., Rapid chromatogrphic purification of DPP-IV in human submaxillary gland (1980) J. Chromatography, 189, p. 233; Liang-Yi, W., Chi-Chang, J., Lucy Sun, H., Yung-Pei, H., Pei-Hsuan, H., Low-Tone, H., Green tea supplementation ameliorates insulin resistance and increases glucose transporter IV content in a fructose-fed rat model (2004) Eur J Nutr, 43, p. 116; Kannappan, S., Jayaraman, T., Rajasekar, P., Ravichandran, M.K., Anuradha, C.V., Cinnamon bark extract improves glucose metabolism and lipid profile in the fructose-fed rat (2006) Singapore Med J, 47 (10), p. 858; Shimizu, S., Ugi, S., Maegawa, H., Egawa, K., Nishio, Y., Yoshizaki, T., Shi, K., Nemoto, K., Protein-tyrosine phosphatase I B as new activator for hepatic lipogenesis via sterol regulatory element-binding protein-1 gene expression (2003) J Biol Chem, 278, p. 43095; Faure, P., Rossinoc, E., Wiernsperger, N., Richard, M.J., Favier, A., Halimi, S., Vitamin E improves the free radical defense system potential and insulin sensitivity of rats fed high fructose diets (1997) J Nutr, 127, p. 103; Mahesh, T., Menon Venugopal, P., Quercetin alleviates oxidative stress in streptozotocin-induced diabetic rats (2004) Phytother Res, 18, p. 123; Vessal, M., Hemmati, M., Vasei, M., Antidiabetic effects of quercetin in streptozocin-induced diabetic rats (2003) Comp Biochem Physiol C Toxicol Pharmacol, 135 C (3), p. 357

PY - 2010

Y1 - 2010

N2 - To study the effect and mode of action of water extract (DVW) and polar fraction of ethanol extract (DVE-4) of D. viscosa in high-fructose diet induced insulin resistance in male Wistar rats. D. viscosa's effects were evaluated on a battery of targets involved in glucose homeostasis (in vitro studies). Rats were rendered insulin resistant by feeding 66% (w/w) fructose and 1.1% (v/w) coconut oil mixed with normal pellet diet (NPD) for six weeks. DVW and DVE4 at different doses were administered simultaneously. At the end of the study, blood glucose, oral glucose tolerance test, lipid profile and insulin were estimated and homeostatic model assessment (HOMA) levels were calculated. In addition, enzymatic and nonenzymatic liver antioxidant levels were also estimated. Quantification of biomarker quercetin was done using HPLC. Fructose diet with DVW, DVE-4 significantly reduced blood glucose, serum insulin, HOMA, lipid profiles and significantly improved glucose tolerance and HDL-c levels. In addition, these extract and fraction also decreased oxidative stress by improving endogenous antioxidants. In different bioassays, DVW and DVE-4 inhibited protein tyrosine phosphatase-1B with IC50 65.8 and 54.9 μg/ml respectively and showed partial inhibition of dipeptidyl peptidase-IV. Moreover, DVW and DVE-4, at 10 μg/ml showed 60 and 54.2% binding to peroxisome proliferator-activated receptor-γ. Further, 2.1% (w/w) of quercetin was quantified in bioactive-DVE-4 using HPLC method. The results provide pharmacological evidence of D. viscosa in treatment of prediabetic conditions and these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.

AB - To study the effect and mode of action of water extract (DVW) and polar fraction of ethanol extract (DVE-4) of D. viscosa in high-fructose diet induced insulin resistance in male Wistar rats. D. viscosa's effects were evaluated on a battery of targets involved in glucose homeostasis (in vitro studies). Rats were rendered insulin resistant by feeding 66% (w/w) fructose and 1.1% (v/w) coconut oil mixed with normal pellet diet (NPD) for six weeks. DVW and DVE4 at different doses were administered simultaneously. At the end of the study, blood glucose, oral glucose tolerance test, lipid profile and insulin were estimated and homeostatic model assessment (HOMA) levels were calculated. In addition, enzymatic and nonenzymatic liver antioxidant levels were also estimated. Quantification of biomarker quercetin was done using HPLC. Fructose diet with DVW, DVE-4 significantly reduced blood glucose, serum insulin, HOMA, lipid profiles and significantly improved glucose tolerance and HDL-c levels. In addition, these extract and fraction also decreased oxidative stress by improving endogenous antioxidants. In different bioassays, DVW and DVE-4 inhibited protein tyrosine phosphatase-1B with IC50 65.8 and 54.9 μg/ml respectively and showed partial inhibition of dipeptidyl peptidase-IV. Moreover, DVW and DVE-4, at 10 μg/ml showed 60 and 54.2% binding to peroxisome proliferator-activated receptor-γ. Further, 2.1% (w/w) of quercetin was quantified in bioactive-DVE-4 using HPLC method. The results provide pharmacological evidence of D. viscosa in treatment of prediabetic conditions and these effects may be mediated by interacting with multiple targets operating in diabetes mellitus.

M3 - Article

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JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 8

ER -