Antifungal effect of cow's urine distillate on Candida species

Jian Meng Hoh, B Dhanashree

Research output: Contribution to journalArticle

Abstract

BACKGROUND: Increase in resistance of Candida species, to routinely used antifungal agents has necessitated the quest for new drugs. Few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. However there is no published report on antifungal effects of cow's urine on clinical Candida isolates.

OBJECTIVE: The present study aims at exploring the antifungal potential of cow's urine on clinical isolates of Candida species.

MATERIAL & METHODS: In this in-vitro experimental study four standard strains and 37 clinical isolates of Candida species were tested for their susceptibility to amphotericin B, fluconazole and voriconazole, by disk diffusion method. Detection of MIC of cow's urine for the Candida isolates was done by agar dilution method using 20-50% concentration of cow's urine.

RESULTS: Clinical isolates of Candida albicans n = 22 (59.5%) Candida glabrata n = 6 (16.2%), Candida tropicalis n = 3 (8.1%) and other Candida species n = 6 were tested for their antifungal susceptibility. Among them, 18.9% were resistant to voriconazole, 24.3% to amphotericin B and 35.1% to fluconazole. Statistically significant association was observed between susceptibility of voriconazole and that of cow's urine (p = 0.045). C.albicans ATCC14053, Candida parapsilosis ATCC22019 and 75.7% of clinical isolates of Candida were susceptible to cow's urine.

CONCLUSIONS: Cow's urine distillate has concentration dependent inhibitory effect on Candida species and is effective on the isolates that are either resistant or sensitive to the routinely used antifungal agents.

Original languageEnglish
JournalJournal of Ayurveda and Integrative Medicine
DOIs
Publication statusE-pub ahead of print - 30-08-2017

Fingerprint

Candida
Urine
Fluconazole
Antifungal Agents
Amphotericin B
Candida albicans
Candida tropicalis
Candida glabrata
Agar
Fungi
Growth
Pharmaceutical Preparations
Voriconazole

Cite this

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title = "Antifungal effect of cow's urine distillate on Candida species",
abstract = "BACKGROUND: Increase in resistance of Candida species, to routinely used antifungal agents has necessitated the quest for new drugs. Few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. However there is no published report on antifungal effects of cow's urine on clinical Candida isolates.OBJECTIVE: The present study aims at exploring the antifungal potential of cow's urine on clinical isolates of Candida species.MATERIAL & METHODS: In this in-vitro experimental study four standard strains and 37 clinical isolates of Candida species were tested for their susceptibility to amphotericin B, fluconazole and voriconazole, by disk diffusion method. Detection of MIC of cow's urine for the Candida isolates was done by agar dilution method using 20-50{\%} concentration of cow's urine.RESULTS: Clinical isolates of Candida albicans n = 22 (59.5{\%}) Candida glabrata n = 6 (16.2{\%}), Candida tropicalis n = 3 (8.1{\%}) and other Candida species n = 6 were tested for their antifungal susceptibility. Among them, 18.9{\%} were resistant to voriconazole, 24.3{\%} to amphotericin B and 35.1{\%} to fluconazole. Statistically significant association was observed between susceptibility of voriconazole and that of cow's urine (p = 0.045). C.albicans ATCC14053, Candida parapsilosis ATCC22019 and 75.7{\%} of clinical isolates of Candida were susceptible to cow's urine.CONCLUSIONS: Cow's urine distillate has concentration dependent inhibitory effect on Candida species and is effective on the isolates that are either resistant or sensitive to the routinely used antifungal agents.",
author = "Hoh, {Jian Meng} and B Dhanashree",
note = "Copyright {\circledC} 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Published by Elsevier B.V. All rights reserved.",
year = "2017",
month = "8",
day = "30",
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Antifungal effect of cow's urine distillate on Candida species. / Hoh, Jian Meng; Dhanashree, B.

In: Journal of Ayurveda and Integrative Medicine, 30.08.2017.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antifungal effect of cow's urine distillate on Candida species

AU - Hoh, Jian Meng

AU - Dhanashree, B

N1 - Copyright © 2017 Transdisciplinary University, Bangalore and World Ayurveda Foundation. Published by Elsevier B.V. All rights reserved.

PY - 2017/8/30

Y1 - 2017/8/30

N2 - BACKGROUND: Increase in resistance of Candida species, to routinely used antifungal agents has necessitated the quest for new drugs. Few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. However there is no published report on antifungal effects of cow's urine on clinical Candida isolates.OBJECTIVE: The present study aims at exploring the antifungal potential of cow's urine on clinical isolates of Candida species.MATERIAL & METHODS: In this in-vitro experimental study four standard strains and 37 clinical isolates of Candida species were tested for their susceptibility to amphotericin B, fluconazole and voriconazole, by disk diffusion method. Detection of MIC of cow's urine for the Candida isolates was done by agar dilution method using 20-50% concentration of cow's urine.RESULTS: Clinical isolates of Candida albicans n = 22 (59.5%) Candida glabrata n = 6 (16.2%), Candida tropicalis n = 3 (8.1%) and other Candida species n = 6 were tested for their antifungal susceptibility. Among them, 18.9% were resistant to voriconazole, 24.3% to amphotericin B and 35.1% to fluconazole. Statistically significant association was observed between susceptibility of voriconazole and that of cow's urine (p = 0.045). C.albicans ATCC14053, Candida parapsilosis ATCC22019 and 75.7% of clinical isolates of Candida were susceptible to cow's urine.CONCLUSIONS: Cow's urine distillate has concentration dependent inhibitory effect on Candida species and is effective on the isolates that are either resistant or sensitive to the routinely used antifungal agents.

AB - BACKGROUND: Increase in resistance of Candida species, to routinely used antifungal agents has necessitated the quest for new drugs. Few studies have revealed that cow's urine can suppress the growth of pathogenic fungi. However there is no published report on antifungal effects of cow's urine on clinical Candida isolates.OBJECTIVE: The present study aims at exploring the antifungal potential of cow's urine on clinical isolates of Candida species.MATERIAL & METHODS: In this in-vitro experimental study four standard strains and 37 clinical isolates of Candida species were tested for their susceptibility to amphotericin B, fluconazole and voriconazole, by disk diffusion method. Detection of MIC of cow's urine for the Candida isolates was done by agar dilution method using 20-50% concentration of cow's urine.RESULTS: Clinical isolates of Candida albicans n = 22 (59.5%) Candida glabrata n = 6 (16.2%), Candida tropicalis n = 3 (8.1%) and other Candida species n = 6 were tested for their antifungal susceptibility. Among them, 18.9% were resistant to voriconazole, 24.3% to amphotericin B and 35.1% to fluconazole. Statistically significant association was observed between susceptibility of voriconazole and that of cow's urine (p = 0.045). C.albicans ATCC14053, Candida parapsilosis ATCC22019 and 75.7% of clinical isolates of Candida were susceptible to cow's urine.CONCLUSIONS: Cow's urine distillate has concentration dependent inhibitory effect on Candida species and is effective on the isolates that are either resistant or sensitive to the routinely used antifungal agents.

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DO - 10.1016/j.jaim.2017.04.009

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JO - Journal of Ayurveda and Integrative Medicine

JF - Journal of Ayurveda and Integrative Medicine

SN - 0975-9476

ER -