Antifungal susceptibility and biofilm production of candida spp. Isolated from clinical samples

Munmun B. Marak, Biranthabail Dhanashree

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.

Original languageEnglish
Article number7495218
JournalInternational Journal of Microbiology
Volume2018
DOIs
Publication statusPublished - 01-01-2018

Fingerprint

Biofilms
Candida
Suppuration
Urine
Dimercaprol
Fluconazole
Candidiasis
Suction
Body Fluids
Amphotericin B
Sputum
Urinary Tract
Bile
Diabetes Mellitus
Age Groups
Demography
Anti-Bacterial Agents
Wounds and Injuries
Pharmaceutical Preparations

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Microbiology (medical)

Cite this

@article{b7b26b8534ec45b19c02dadcb7fe654b,
title = "Antifungal susceptibility and biofilm production of candida spp. Isolated from clinical samples",
abstract = "Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5{\%}) followed by C. tropicalis (28.88{\%}), C. krusei (20{\%}), C. glabrata (3.33{\%}), and C. parapsilosis (2.22{\%}). Candida spp. were isolated from urine (43{\%}), BAL/sputum (18.88{\%}), high vaginal swab (8.88{\%}), suction tips (7.77{\%}), blood and wound swabs (6.66{\%}), pus (3.33{\%}), bile aspirate (2.22{\%}), and deep tissue (1.11{\%}). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100{\%} sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11{\%} susceptibility. 24.44{\%} of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.",
author = "Marak, {Munmun B.} and Biranthabail Dhanashree",
year = "2018",
month = "1",
day = "1",
doi = "10.1155/2018/7495218",
language = "English",
volume = "2018",
journal = "International Journal of Microbiology",
issn = "1687-918X",
publisher = "Hindawi Publishing Corporation",

}

Antifungal susceptibility and biofilm production of candida spp. Isolated from clinical samples. / Marak, Munmun B.; Dhanashree, Biranthabail.

In: International Journal of Microbiology, Vol. 2018, 7495218, 01.01.2018.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antifungal susceptibility and biofilm production of candida spp. Isolated from clinical samples

AU - Marak, Munmun B.

AU - Dhanashree, Biranthabail

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.

AB - Objective. The study aims to speciate clinical Candida isolates and detect their biofilm-forming ability and antifungal resistance. Methods. All the Candida spp. isolated from different clinical samples like pus, urine, blood, and body fluid were included in the study. Biofilm production was tested by the microtiter plate method. Antifungal susceptibility was studied by the disk diffusion method. Patient's demographic details such as age, sex, and clinical information were collected. Presence of other risk factors such as diabetes mellitus, history of antibiotic use, and any urinary tract instrumentations was also recorded. Results. Among 90 Candida species isolated, most predominant species was found to be C. albicans (45.5%) followed by C. tropicalis (28.88%), C. krusei (20%), C. glabrata (3.33%), and C. parapsilosis (2.22%). Candida spp. were isolated from urine (43%), BAL/sputum (18.88%), high vaginal swab (8.88%), suction tips (7.77%), blood and wound swabs (6.66%), pus (3.33%), bile aspirate (2.22%), and deep tissue (1.11%). A larger number of females were affected than males, and the age group of 51 to 60 years was more susceptible to candidiasis. A higher number of C. albicans isolates produced biofilm followed by C. parapsilosis, C. tropicalis, and C. krusei. However, C. glabrata showed no biofilm production in our study. All Candida isolates were 100% sensitive to amphotericin B. Voriconazole was the next effective drug with 81.11% susceptibility. 24.44% of strains were resistant to fluconazole. Conclusion. Speciation of Candida isolates, detection of ability to form the biofilm, and monitoring of antifungal susceptibility testing are necessary for appropriate treatment.

UR - http://www.scopus.com/inward/record.url?scp=85056606612&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85056606612&partnerID=8YFLogxK

U2 - 10.1155/2018/7495218

DO - 10.1155/2018/7495218

M3 - Article

AN - SCOPUS:85056606612

VL - 2018

JO - International Journal of Microbiology

JF - International Journal of Microbiology

SN - 1687-918X

M1 - 7495218

ER -