Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal

Supram Hosuru Subramanya, Bharat Prasad Baral, Nawal Kishor Sharan, Niranjan Nayak, Yang Metok, Brijesh Sathian, Indira Bairy, Shishir Gokhale

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Objective: Candida species are part of the commensal microflora in many anatomical sites of the human body; however, breach in the integrity of the body part and impaired immunity of the host can lead to invasive candidiasis. A number of virulence determinants could contribute towards its pathogenicity. Thus we attempted to evaluate the in vitro expression of different virulence factors among clinical isolates of Candida species and assayed their susceptibility patterns against a range of antifungal agents. Result: Of the total of 71 isolates we obtained, 48 (67.6%) were Candida albicans, 11 (15.49%) Candida tropicalis, 09 (12.67%) Candida glabrata and 03 (4.22%) were Candida krusei. Proteinase, phospholipase and esterase production could be revealed amongst 43 (60.56%), 44 (61.97%) and 49 (69.01%) isolates respectively. None of the isolates showed DNAase activity. Fifty-five (77.39%) isolates were biofilm producers, and 53 (74.6%) exhibited high cell surface hydrophobicity.

Original languageEnglish
Article number543
JournalBMC Research Notes
Volume10
Issue number1
DOIs
Publication statusPublished - 02-11-2017

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Nepal
Candida
Virulence
Human Body
Invasive Candidiasis
Candida tropicalis
Candida glabrata
Deoxyribonucleases
Phospholipases
Antifungal Agents
Virulence Factors
Esterases
Biofilms
Candida albicans
Hydrophobic and Hydrophilic Interactions
Immunity
Peptide Hydrolases
Hydrophobicity

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Subramanya, S. H., Baral, B. P., Sharan, N. K., Nayak, N., Metok, Y., Sathian, B., ... Gokhale, S. (2017). Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal. BMC Research Notes, 10(1), [543]. https://doi.org/10.1186/s13104-017-2852-x
Subramanya, Supram Hosuru ; Baral, Bharat Prasad ; Sharan, Nawal Kishor ; Nayak, Niranjan ; Metok, Yang ; Sathian, Brijesh ; Bairy, Indira ; Gokhale, Shishir. / Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal. In: BMC Research Notes. 2017 ; Vol. 10, No. 1.
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abstract = "Objective: Candida species are part of the commensal microflora in many anatomical sites of the human body; however, breach in the integrity of the body part and impaired immunity of the host can lead to invasive candidiasis. A number of virulence determinants could contribute towards its pathogenicity. Thus we attempted to evaluate the in vitro expression of different virulence factors among clinical isolates of Candida species and assayed their susceptibility patterns against a range of antifungal agents. Result: Of the total of 71 isolates we obtained, 48 (67.6{\%}) were Candida albicans, 11 (15.49{\%}) Candida tropicalis, 09 (12.67{\%}) Candida glabrata and 03 (4.22{\%}) were Candida krusei. Proteinase, phospholipase and esterase production could be revealed amongst 43 (60.56{\%}), 44 (61.97{\%}) and 49 (69.01{\%}) isolates respectively. None of the isolates showed DNAase activity. Fifty-five (77.39{\%}) isolates were biofilm producers, and 53 (74.6{\%}) exhibited high cell surface hydrophobicity.",
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Subramanya, SH, Baral, BP, Sharan, NK, Nayak, N, Metok, Y, Sathian, B, Bairy, I & Gokhale, S 2017, 'Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal', BMC Research Notes, vol. 10, no. 1, 543. https://doi.org/10.1186/s13104-017-2852-x

Antifungal susceptibility and phenotypic virulence markers of Candida species isolated from Nepal. / Subramanya, Supram Hosuru; Baral, Bharat Prasad; Sharan, Nawal Kishor; Nayak, Niranjan; Metok, Yang; Sathian, Brijesh; Bairy, Indira; Gokhale, Shishir.

In: BMC Research Notes, Vol. 10, No. 1, 543, 02.11.2017.

Research output: Contribution to journalArticle

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AU - Subramanya, Supram Hosuru

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AU - Sharan, Nawal Kishor

AU - Nayak, Niranjan

AU - Metok, Yang

AU - Sathian, Brijesh

AU - Bairy, Indira

AU - Gokhale, Shishir

PY - 2017/11/2

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