Antigen identification using skin deficient in basement-membrane protein

A novel tool for the diagnosis of subepidermal immunobullous diseases

R. Rao, B. Bhogal, R. Groves

Research output: Contribution to journalReview article

3 Citations (Scopus)

Abstract

Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate. Click here for the corresponding questions to this CME article.

Original languageEnglish
Pages (from-to)289-294
Number of pages6
JournalClinical and Experimental Dermatology
Volume38
Issue number3
DOIs
Publication statusPublished - 01-04-2013

Fingerprint

Staphylococcal Protein A
Immunoblotting
Basement Membrane
Membrane Proteins
Epidermolysis Bullosa
Antigens
Skin
Blister
Indirect Fluorescent Antibody Technique
Fluorescent Antibody Technique

All Science Journal Classification (ASJC) codes

  • Dermatology

Cite this

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Antigen identification using skin deficient in basement-membrane protein : A novel tool for the diagnosis of subepidermal immunobullous diseases. / Rao, R.; Bhogal, B.; Groves, R.

In: Clinical and Experimental Dermatology, Vol. 38, No. 3, 01.04.2013, p. 289-294.

Research output: Contribution to journalReview article

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