Abstract
Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate. Click here for the corresponding questions to this CME article.
| Original language | English |
|---|---|
| Pages (from-to) | 289-294 |
| Number of pages | 6 |
| Journal | Clinical and Experimental Dermatology |
| Volume | 38 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - 01-04-2013 |
Fingerprint
All Science Journal Classification (ASJC) codes
- Dermatology
Cite this
}
Antigen identification using skin deficient in basement-membrane protein : A novel tool for the diagnosis of subepidermal immunobullous diseases. / Rao, R.; Bhogal, B.; Groves, R.
In: Clinical and Experimental Dermatology, Vol. 38, No. 3, 01.04.2013, p. 289-294.Research output: Contribution to journal › Review article
TY - JOUR
T1 - Antigen identification using skin deficient in basement-membrane protein
T2 - A novel tool for the diagnosis of subepidermal immunobullous diseases
AU - Rao, R.
AU - Bhogal, B.
AU - Groves, R.
PY - 2013/4/1
Y1 - 2013/4/1
N2 - Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate. Click here for the corresponding questions to this CME article.
AB - Common unifying features of the subepidermal blistering diseases are the presence of tense blisters clinically and demonstration by immunofluorescence of linear deposition of immunoreactants along the dermoepidermal junction. Further characterization of subtype is possible by identification of the target antigen by immunoblotting. However, immunoblotting is time-consuming and may not be practical for routine use in the laboratory. In this report, we describe a simple technique to identify the target antigen by indirect immunofluorescence, using epidermolysis bullosa skin as substrate. Click here for the corresponding questions to this CME article.
UR - http://www.scopus.com/inward/record.url?scp=84875304569&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84875304569&partnerID=8YFLogxK
U2 - 10.1111/ced.12115
DO - 10.1111/ced.12115
M3 - Review article
C2 - 23517360
AN - SCOPUS:84875304569
VL - 38
SP - 289
EP - 294
JO - Clinical and Experimental Dermatology
JF - Clinical and Experimental Dermatology
SN - 0307-6938
IS - 3
ER -