Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride

E. Kasi Viswanadh, B. Nageshwar Rao, B. S. Satish Rao

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Cadmium is an environmental metal toxin implicated in human diseases. Mangiferin (MGN), a naturally occurring glucosylxanthone, is present in Mangifera indica. In this study, the protective role of MGN against cadmium chloride (CdCl2)-induced genotoxicity was studied in Swiss albino mice. Mice were administered with single intra-peritoneal (i.p.) optimal dose of MGN (2.5 mg/kg b.wt.) before treatment with various concentrations of CdCl 2 (7, 8, 9, 10 and 11 mg/kg b.wt.). The LD50(30) was found to be 8.5 mg/kg b.wt. for DDW + CdCl2 group, while it was increased to 9.77 mg/kg after MGN treatment resulting in increase in the LD 50(30) value by 1.26 mg, with a dose reduction factor (DRF) of 1.14. Treatment of mice to various doses of CdCl2 resulted in a dose-dependent increase in the frequency of micronucleated polychromatic (MnPCE) and normochromatic erythrocytes (MnNCE), with corresponding decrease in the polychromatic / normochromatic erythrocyte ratio (PCE/NCE ratio) at various post-treatment times. MGN (2.5 mg/kg b.wt.) pretreatment significantly (p <.001) reduced the frequency of MnPCE, MnNCE and increased PCE/NCE ratio when compared with the DDW + CdCl2 group at all post-treatment times indicating its antigenotoxic effect. Further, pretreatment of MGN declined the lipid peroxidation (LPx) content in liver, whereas significant increase was observed in hepatic Glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity. Our study revealed that MGN has potent antigenotoxic effect against CdCl2-induced toxicity in mice, which may be due to the scavenging of free radicals and increased antioxidant status.

Original languageEnglish
Pages (from-to)409-418
Number of pages10
JournalHuman and Experimental Toxicology
Volume29
Issue number5
DOIs
Publication statusPublished - 05-2010

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Cadmium Chloride
Antioxidants
Enzymes
Erythrocytes
Mangifera
Liver
Lethal Dose 50
Scavenging
mangiferin
Glutathione Transferase
Cadmium
Catalase
Lipid Peroxidation
Superoxide Dismutase
Free Radicals
Glutathione
Toxicity
Metals
Lipids

All Science Journal Classification (ASJC) codes

  • Toxicology
  • Health, Toxicology and Mutagenesis

Cite this

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title = "Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride",
abstract = "Cadmium is an environmental metal toxin implicated in human diseases. Mangiferin (MGN), a naturally occurring glucosylxanthone, is present in Mangifera indica. In this study, the protective role of MGN against cadmium chloride (CdCl2)-induced genotoxicity was studied in Swiss albino mice. Mice were administered with single intra-peritoneal (i.p.) optimal dose of MGN (2.5 mg/kg b.wt.) before treatment with various concentrations of CdCl 2 (7, 8, 9, 10 and 11 mg/kg b.wt.). The LD50(30) was found to be 8.5 mg/kg b.wt. for DDW + CdCl2 group, while it was increased to 9.77 mg/kg after MGN treatment resulting in increase in the LD 50(30) value by 1.26 mg, with a dose reduction factor (DRF) of 1.14. Treatment of mice to various doses of CdCl2 resulted in a dose-dependent increase in the frequency of micronucleated polychromatic (MnPCE) and normochromatic erythrocytes (MnNCE), with corresponding decrease in the polychromatic / normochromatic erythrocyte ratio (PCE/NCE ratio) at various post-treatment times. MGN (2.5 mg/kg b.wt.) pretreatment significantly (p <.001) reduced the frequency of MnPCE, MnNCE and increased PCE/NCE ratio when compared with the DDW + CdCl2 group at all post-treatment times indicating its antigenotoxic effect. Further, pretreatment of MGN declined the lipid peroxidation (LPx) content in liver, whereas significant increase was observed in hepatic Glutathione (GSH), glutathione-S-transferase (GST), superoxide dismutase (SOD) and catalase (CAT) activity. Our study revealed that MGN has potent antigenotoxic effect against CdCl2-induced toxicity in mice, which may be due to the scavenging of free radicals and increased antioxidant status.",
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Antigenotoxic effect of mangiferin and changes in antioxidant enzyme levels of Swiss albino mice treated with cadmium chloride. / Kasi Viswanadh, E.; Nageshwar Rao, B.; Satish Rao, B. S.

In: Human and Experimental Toxicology, Vol. 29, No. 5, 05.2010, p. 409-418.

Research output: Contribution to journalArticle

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