Antihepatotoxic effect of grape seed oil in rat

M. Uma Maheswari, P.G.M. Rao

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Abstract

Objectives: To study the effect of oral administration of grape seed oil (GSO) against carbontetrachloride (CCl4)-induced hepatotoxicity in rats. Methods: Liver damage was induced in male Wistar rats (150-250 g) by administering CCl4 (0.5 ml/kg, i.p.) once per day for 7 days and the extent of damage was studied by assessing biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum and concentrations of malondialdehyde (MDA), hydroperoxides, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein (TP) in liver. The effect of co-administration of GSO (3.7 g/kg, orally) on the above parameters was further investigated and compared with a vitamin E (100 mg/kg, orally) treated group. Histopatholgical studies of the experimental animals were also done. Results: Oral administration of GSO (3.7 g/kg, body weight orally) for 7 days resulted in a significant reduction in serum AST, ALT, and ALP levels and liver MDA and hydroperoxides and significant improvement in glutathione, SOD, CAT, and TP, when compared with CCl4 damaged rats. The antioxidant effect of GSO at 3.7 g/kg for 7 days was found to be comparable with vitamin E (100 mg/kg, orally) in CCl4-treated rats. Profound fatty degeneration, fibrosis, and necrosis observed in the hepatic architecture of CCl4-treated rats were found to acquire near - normalcy in drug co-administered rats. Conclusion: The GSO has protected the liver from CCl4 damage. Probable mechanism of action may be due to the protection against oxidative damage produced by CCl4.
Original languageEnglish
Pages (from-to)179-182
Number of pages4
JournalIndian Journal of Pharmacology
Volume37
Issue number3
Publication statusPublished - 2005

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Vitis
Seeds
Oils
Liver
Aspartate Aminotransferases
Malondialdehyde
Alanine Transaminase
Vitamin E
Catalase
Hydrogen Peroxide
Superoxide Dismutase
Glutathione
Alkaline Phosphatase
Oral Administration
Serum
Wistar Rats
Proteins
Fibrosis
Necrosis
Antioxidants

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Uma Maheswari, M., & Rao, P. G. M. (2005). Antihepatotoxic effect of grape seed oil in rat. Indian Journal of Pharmacology, 37(3), 179-182.
Uma Maheswari, M. ; Rao, P.G.M. / Antihepatotoxic effect of grape seed oil in rat. In: Indian Journal of Pharmacology. 2005 ; Vol. 37, No. 3. pp. 179-182.
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abstract = "Objectives: To study the effect of oral administration of grape seed oil (GSO) against carbontetrachloride (CCl4)-induced hepatotoxicity in rats. Methods: Liver damage was induced in male Wistar rats (150-250 g) by administering CCl4 (0.5 ml/kg, i.p.) once per day for 7 days and the extent of damage was studied by assessing biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum and concentrations of malondialdehyde (MDA), hydroperoxides, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein (TP) in liver. The effect of co-administration of GSO (3.7 g/kg, orally) on the above parameters was further investigated and compared with a vitamin E (100 mg/kg, orally) treated group. Histopatholgical studies of the experimental animals were also done. Results: Oral administration of GSO (3.7 g/kg, body weight orally) for 7 days resulted in a significant reduction in serum AST, ALT, and ALP levels and liver MDA and hydroperoxides and significant improvement in glutathione, SOD, CAT, and TP, when compared with CCl4 damaged rats. The antioxidant effect of GSO at 3.7 g/kg for 7 days was found to be comparable with vitamin E (100 mg/kg, orally) in CCl4-treated rats. Profound fatty degeneration, fibrosis, and necrosis observed in the hepatic architecture of CCl4-treated rats were found to acquire near - normalcy in drug co-administered rats. Conclusion: The GSO has protected the liver from CCl4 damage. Probable mechanism of action may be due to the protection against oxidative damage produced by CCl4.",
author = "{Uma Maheswari}, M. and P.G.M. Rao",
note = "Cited By :26 Export Date: 10 November 2017 CODEN: INJPD Correspondence Address: Uma Maheswari, M.; College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamilnadu, India; email: muma_prakash@yahoo.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; aminotransferase, 9031-66-7; carbon tetrachloride, 56-23-5; catalase, 9001-05-2; glutathione, 70-18-8; malonaldehyde, 542-78-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1 Manufacturers: loduca bros, United States References: Natella, F., Belleli, F., Gentili, V., Ursini, F., Scaccini, C., Grape seed proanthocyanidins prevent plasma post prandial oxidative stress in humans (2002) J Agric Food Chem, 26, pp. 7720-7725; Nash, D.T., Nash, S.D., Grapeseed oil, a natural agent which raises serum HDL levels (1993) J Am Coll Cardiol, 21, pp. 318-320; Bagchi, D., Bagchi, M., Stohs, S., Ray, S.D., Sen, C.K., Preuss, H.G., Cellular protection with proanthocyanidins derived from grape seeds (2002) Ann NY Acad Sci, 957, pp. 260-270; Bagchi, D., Ray, S.D., Bagchi, M., Preuss, H.G., Stohs, S.J., Mechanistic pathways of antioxidant cytoprotection by a novel IH 636 grape seed proanthocyanidin extract (2002) Indian J Exp Biol, 6, pp. 717-726; Deleve, L.D., Kaplowitz, N., Mechanisms of drug induced liver diseases (1995) Gastroenterol Clin N Am, 24, pp. 787-810; Bhat, A.D., Bhat, S., Indigenous drugs and liver diseases (1996) Indian J Gastroenterol, 15, pp. 63-67; Paget, G.E., Barnes, J.M., (1964) Pharmacometrics, 1. , Evaluation of drug activities. Lawrence DR, Bachrach AL, editors. New York: Academic Press;; Acute oral Toxicity - Acute oral toxic class method (2000) Eleventh Addendum to the OECD Guidelines for the Testing of Chemicals, , Guideline 423, adopted 23.03.1996. Organisation for Economic Co-Operation and Development, Paris, June 2000; Rao, P.G.M., Rao, S.G., Kumar, V., Effect of Hepatogard against carbontetrachloride induced liver damage in rats (1993) Fitoterapia, 64, pp. 108-113; Dwivedi, Y., Rastogi, R., Chander, R., Sharma, S.K., Kapoor, N.K., Garg, N.K., Hepatoprotective activity of Picroliv against carbontetrachloride induced liver damage in rats (1990) Indian J Med Res, 92, pp. 195-200; Kataria, M., Singh, L.N., Hepatoprotective activity of Liv 52 and Kumaryasava on carbontetrachloride induced hepatic damage in rats (1997) Indian J Exp Biol, 35, pp. 655-657; Sheweita, S.A., Abd El-Gabar, M., Bastawy, M., Carbon tetrachloride induced changes in the activity of Phase II drug metabolizing enzymes (2001) Toxicology, 165, pp. 217-224; Reitman, S., Frankel, S., A colorimetric method for the determination of SGOT and SGPT (1957) Am J Clin Pathol, 28, pp. 56-63; Kind, P.R.N., Kings, E.J., Estimation of plasma phosphatase by determination of hydrolysed phenol with antipyrine (1954) J Clin Pathol, 7, pp. 322-330; Ellaman, G.L., Tissue sulfhydryl group (1959) Arch Biochem Biophys, 82, pp. 70-72; Yagi, K., Rastogi, R., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Analytical Biochem, 95, pp. 351-358; Jiang, Zy., Hunt, J.V., Wolff, S.P., Ferrous ion oxidation in the presence of Xylenol orange for detection of lipid hydroxide in low-density lipoprotein (1992) Anal Biochem, 202, pp. 384-389; Kakkar, P., Dass, B., Visvanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1972) Indian J Biochem, 197, pp. 588-590; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Lowry, O.H., Rosenberg, N.J., Farr, A.L., Randall, R.J., Protein measurement with Folin Phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Prophet, E.B., Mills, B., Arrington, J.B., Sobin, L.H., (1992) Laboratory Methods in Histotechnology, , Washington DC: Armed Forces Institute of Pathology;; Bancroft, J.D., Stevens, A., Dawson, I.M.S., (1977) Theory and Practice of Histological T Echniques, , Edinburgh, London, New York. Churchill-Livingstone; Armitate, P., Bregg, G., (1985) Statistical Methods in Medical Research. 2nd Ed., , London: Blackwell Scientific Publications;; Johnson, D.E., Kroening, C., Mechanism of early carbontetrchloride toxicity in cultured rat hepatocytes (1998) Pharmacol Toxicol, 83, pp. 231-239; Srivastava, S.P., Chen, N.O., Holtzman, J.L., The in vitro NADPH dependent inhibition by CCl4 of the ATP dependent calcium uptake of hepatic microsomes from male rats. Studies on the mechanism of inactivation of the hepatic microsomal calcium pump by the CCl3 radical (1990) J Biol Chem, 265, pp. 8392-8399",
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Uma Maheswari, M & Rao, PGM 2005, 'Antihepatotoxic effect of grape seed oil in rat', Indian Journal of Pharmacology, vol. 37, no. 3, pp. 179-182.

Antihepatotoxic effect of grape seed oil in rat. / Uma Maheswari, M.; Rao, P.G.M.

In: Indian Journal of Pharmacology, Vol. 37, No. 3, 2005, p. 179-182.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antihepatotoxic effect of grape seed oil in rat

AU - Uma Maheswari, M.

AU - Rao, P.G.M.

N1 - Cited By :26 Export Date: 10 November 2017 CODEN: INJPD Correspondence Address: Uma Maheswari, M.; College of Pharmacy, Sri Ramakrishna Institute of Paramedical Sciences, Coimbatore, Tamilnadu, India; email: muma_prakash@yahoo.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alkaline phosphatase, 9001-78-9; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; aminotransferase, 9031-66-7; carbon tetrachloride, 56-23-5; catalase, 9001-05-2; glutathione, 70-18-8; malonaldehyde, 542-78-9; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1 Manufacturers: loduca bros, United States References: Natella, F., Belleli, F., Gentili, V., Ursini, F., Scaccini, C., Grape seed proanthocyanidins prevent plasma post prandial oxidative stress in humans (2002) J Agric Food Chem, 26, pp. 7720-7725; Nash, D.T., Nash, S.D., Grapeseed oil, a natural agent which raises serum HDL levels (1993) J Am Coll Cardiol, 21, pp. 318-320; Bagchi, D., Bagchi, M., Stohs, S., Ray, S.D., Sen, C.K., Preuss, H.G., Cellular protection with proanthocyanidins derived from grape seeds (2002) Ann NY Acad Sci, 957, pp. 260-270; Bagchi, D., Ray, S.D., Bagchi, M., Preuss, H.G., Stohs, S.J., Mechanistic pathways of antioxidant cytoprotection by a novel IH 636 grape seed proanthocyanidin extract (2002) Indian J Exp Biol, 6, pp. 717-726; Deleve, L.D., Kaplowitz, N., Mechanisms of drug induced liver diseases (1995) Gastroenterol Clin N Am, 24, pp. 787-810; Bhat, A.D., Bhat, S., Indigenous drugs and liver diseases (1996) Indian J Gastroenterol, 15, pp. 63-67; Paget, G.E., Barnes, J.M., (1964) Pharmacometrics, 1. , Evaluation of drug activities. Lawrence DR, Bachrach AL, editors. New York: Academic Press;; Acute oral Toxicity - Acute oral toxic class method (2000) Eleventh Addendum to the OECD Guidelines for the Testing of Chemicals, , Guideline 423, adopted 23.03.1996. Organisation for Economic Co-Operation and Development, Paris, June 2000; Rao, P.G.M., Rao, S.G., Kumar, V., Effect of Hepatogard against carbontetrachloride induced liver damage in rats (1993) Fitoterapia, 64, pp. 108-113; Dwivedi, Y., Rastogi, R., Chander, R., Sharma, S.K., Kapoor, N.K., Garg, N.K., Hepatoprotective activity of Picroliv against carbontetrachloride induced liver damage in rats (1990) Indian J Med Res, 92, pp. 195-200; Kataria, M., Singh, L.N., Hepatoprotective activity of Liv 52 and Kumaryasava on carbontetrachloride induced hepatic damage in rats (1997) Indian J Exp Biol, 35, pp. 655-657; Sheweita, S.A., Abd El-Gabar, M., Bastawy, M., Carbon tetrachloride induced changes in the activity of Phase II drug metabolizing enzymes (2001) Toxicology, 165, pp. 217-224; Reitman, S., Frankel, S., A colorimetric method for the determination of SGOT and SGPT (1957) Am J Clin Pathol, 28, pp. 56-63; Kind, P.R.N., Kings, E.J., Estimation of plasma phosphatase by determination of hydrolysed phenol with antipyrine (1954) J Clin Pathol, 7, pp. 322-330; Ellaman, G.L., Tissue sulfhydryl group (1959) Arch Biochem Biophys, 82, pp. 70-72; Yagi, K., Rastogi, R., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Analytical Biochem, 95, pp. 351-358; Jiang, Zy., Hunt, J.V., Wolff, S.P., Ferrous ion oxidation in the presence of Xylenol orange for detection of lipid hydroxide in low-density lipoprotein (1992) Anal Biochem, 202, pp. 384-389; Kakkar, P., Dass, B., Visvanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1972) Indian J Biochem, 197, pp. 588-590; Sinha, K.A., Colorimetric assay of catalase (1972) Anal Biochem, 47, pp. 389-394; Lowry, O.H., Rosenberg, N.J., Farr, A.L., Randall, R.J., Protein measurement with Folin Phenol reagent (1951) J Biol Chem, 193, pp. 265-275; Prophet, E.B., Mills, B., Arrington, J.B., Sobin, L.H., (1992) Laboratory Methods in Histotechnology, , Washington DC: Armed Forces Institute of Pathology;; Bancroft, J.D., Stevens, A., Dawson, I.M.S., (1977) Theory and Practice of Histological T Echniques, , Edinburgh, London, New York. Churchill-Livingstone; Armitate, P., Bregg, G., (1985) Statistical Methods in Medical Research. 2nd Ed., , London: Blackwell Scientific Publications;; Johnson, D.E., Kroening, C., Mechanism of early carbontetrchloride toxicity in cultured rat hepatocytes (1998) Pharmacol Toxicol, 83, pp. 231-239; Srivastava, S.P., Chen, N.O., Holtzman, J.L., The in vitro NADPH dependent inhibition by CCl4 of the ATP dependent calcium uptake of hepatic microsomes from male rats. Studies on the mechanism of inactivation of the hepatic microsomal calcium pump by the CCl3 radical (1990) J Biol Chem, 265, pp. 8392-8399

PY - 2005

Y1 - 2005

N2 - Objectives: To study the effect of oral administration of grape seed oil (GSO) against carbontetrachloride (CCl4)-induced hepatotoxicity in rats. Methods: Liver damage was induced in male Wistar rats (150-250 g) by administering CCl4 (0.5 ml/kg, i.p.) once per day for 7 days and the extent of damage was studied by assessing biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum and concentrations of malondialdehyde (MDA), hydroperoxides, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein (TP) in liver. The effect of co-administration of GSO (3.7 g/kg, orally) on the above parameters was further investigated and compared with a vitamin E (100 mg/kg, orally) treated group. Histopatholgical studies of the experimental animals were also done. Results: Oral administration of GSO (3.7 g/kg, body weight orally) for 7 days resulted in a significant reduction in serum AST, ALT, and ALP levels and liver MDA and hydroperoxides and significant improvement in glutathione, SOD, CAT, and TP, when compared with CCl4 damaged rats. The antioxidant effect of GSO at 3.7 g/kg for 7 days was found to be comparable with vitamin E (100 mg/kg, orally) in CCl4-treated rats. Profound fatty degeneration, fibrosis, and necrosis observed in the hepatic architecture of CCl4-treated rats were found to acquire near - normalcy in drug co-administered rats. Conclusion: The GSO has protected the liver from CCl4 damage. Probable mechanism of action may be due to the protection against oxidative damage produced by CCl4.

AB - Objectives: To study the effect of oral administration of grape seed oil (GSO) against carbontetrachloride (CCl4)-induced hepatotoxicity in rats. Methods: Liver damage was induced in male Wistar rats (150-250 g) by administering CCl4 (0.5 ml/kg, i.p.) once per day for 7 days and the extent of damage was studied by assessing biochemical parameters such as alanine aminotransferase (ALT), aspartate aminotransferase (AST), and alkaline phosphatase (ALP) in serum and concentrations of malondialdehyde (MDA), hydroperoxides, glutathione (GSH), catalase (CAT), superoxide dismutase (SOD), and total protein (TP) in liver. The effect of co-administration of GSO (3.7 g/kg, orally) on the above parameters was further investigated and compared with a vitamin E (100 mg/kg, orally) treated group. Histopatholgical studies of the experimental animals were also done. Results: Oral administration of GSO (3.7 g/kg, body weight orally) for 7 days resulted in a significant reduction in serum AST, ALT, and ALP levels and liver MDA and hydroperoxides and significant improvement in glutathione, SOD, CAT, and TP, when compared with CCl4 damaged rats. The antioxidant effect of GSO at 3.7 g/kg for 7 days was found to be comparable with vitamin E (100 mg/kg, orally) in CCl4-treated rats. Profound fatty degeneration, fibrosis, and necrosis observed in the hepatic architecture of CCl4-treated rats were found to acquire near - normalcy in drug co-administered rats. Conclusion: The GSO has protected the liver from CCl4 damage. Probable mechanism of action may be due to the protection against oxidative damage produced by CCl4.

M3 - Article

VL - 37

SP - 179

EP - 182

JO - Indian Journal of Pharmacology

JF - Indian Journal of Pharmacology

SN - 0253-7613

IS - 3

ER -