Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats

R. Govindarajan, M. Vijayakumar, Ch.V. Rao, A. Shirwaikar, S. Kumar, A.K.S. Rawat, P. Pushpangadan

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan-induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization. Copyright © 2007 John Wiley & Sons, Ltd.
Original languageEnglish
Pages (from-to)975-979
Number of pages5
JournalPhytotherapy Research
Volume21
Issue number10
DOIs
Publication statusPublished - 2007

Fingerprint

Carrageenan
Flavonoids
ferrous sulfate
Anti-Inflammatory Agents
Antioxidants
Glutathione Peroxidase
Alkaloids
Catalase
Lipid Peroxidation
Superoxide Dismutase
Spleen
Liver
Indomethacin
Glutathione
High Pressure Liquid Chromatography
Pharmaceutical Preparations

Cite this

Govindarajan, R., Vijayakumar, M., Rao, C. V., Shirwaikar, A., Kumar, S., Rawat, A. K. S., & Pushpangadan, P. (2007). Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. Phytotherapy Research, 21(10), 975-979. https://doi.org/10.1002/ptr.2199
Govindarajan, R. ; Vijayakumar, M. ; Rao, Ch.V. ; Shirwaikar, A. ; Kumar, S. ; Rawat, A.K.S. ; Pushpangadan, P. / Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. In: Phytotherapy Research. 2007 ; Vol. 21, No. 10. pp. 975-979.
@article{e1a25d43d99d48e4b266ca51017091d8,
title = "Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats",
abstract = "Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan-induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization. Copyright {\circledC} 2007 John Wiley & Sons, Ltd.",
author = "R. Govindarajan and M. Vijayakumar and Ch.V. Rao and A. Shirwaikar and S. Kumar and A.K.S. Rawat and P. Pushpangadan",
note = "Cited By :22 Export Date: 10 November 2017 CODEN: PHYRE Correspondence Address: Govindarajan, R.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Rana Pratap Marg 226 001, India; email: govind108@yahoo.com Chemicals/CAS: caffeic acid, 27323-69-9, 331-39-5; carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; catalase, 9001-05-2; chlorogenic acid, 327-97-9; ferrous sulfate, 10028-21-4, 10124-49-9, 13463-43-9, 7720-78-7, 7782-63-0; glutathione peroxidase, 9013-66-5; indometacin, 53-86-1, 74252-25-8, 7681-54-1; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Anti-Inflammatory Agents; Antioxidants; Carrageenan, 9000-07-1; Catalase, 1.11.1.6; Desmodium gangeticum extract; Flavonoids; Glutathione Peroxidase, 1.11.1.9; Indomethacin, 53-86-1; Plant Extracts; Superoxide Dismutase, 1.15.1.1 Manufacturers: Sigma, United States References: Al-Qirim, T.M., Shahwan, M., Zaidi, K.R., Uddin, Q., Banu, N., Effect of khat, its constituents and restraint stress in free radical metabolism of rats (2002) J Ethnopharmacol, 83, pp. 245-250; Carlo, G.D., Mascolo, N., Izzo, A.A., Capasso, F., Flavonoids: Old and new aspects of a class of natural therapeutic drugs (1999) Life Sci, 65, pp. 337-353; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, pp. 33-35. , Council of Scientific and Industrial Research: New Delhi; Cohen, G., Dembiec, D., Marcus, J., Measurement of catalase activity in tissue extracts (1970) Anal Biochem, 34, pp. 30-38; Foley, S., Navaratnam, S., David, J.M., Edward, J.L., George, T., Rice-Evans, C.A., Singlet oxygen quenching and the redox properties of hydroxycinnamic acids (1999) Free Radic Biol Med, 26, pp. 1206-1208; Ghosal, S., Bhattacharya, S.K., Desmodium alkaloids II, chemical and pharmacological evaluation of D. gangeticum (1972) Planta Med, 22, pp. 434-440; Ghosh, D., Anandkumar, A., Anti-inflammatory and analgesic activities of gangetin - A pterocarpenoid from Desmodium gangeticum (1981) Indian J Pharmacol, 15, pp. 391-402; Govindarajan, R., Rastogi, S., Vijayakumar, M., Studies on antioxidant activities of Desmodium gangeticum (2003) Biol Pharm Bull, 26, pp. 1424-1427; Govindarajan, R., Vijayakumar, M., Pushpangadan, P., Antioxidant approach to disease management and the role of Rasayana herbs of Ayurveda (2005) J Ethnopharmacol, 99, pp. 165-178; Halliwell, B., Gutteridge, J.M.C., Cross, C.E., Free radicals, antioxidants and human diseases: Where are we now? (1992) J Lab Clin Med, 119, pp. 598-620; Haraguchi, H., Saitao, T., Ishikawa, H., Antiperoxidative components in Thymus vulgaris (1996) Planta Med, 62, pp. 217-221; Harman, D., The aging process: Major risk factor for disease and death (1991) Proc Natl Acad Sci, 88, pp. 5360-5370; Hogg, N., Free radicals in disease (1998) Semin Reprod Endocrinol, 16, pp. 241-248; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Indian J Biochem Biophys, 21, pp. 130-132; Landolfi, R., Mower, R.L., Steiner, M., Modification of platelet function and arachidonic acid metabolism by bioflavonoids: Structure-activity relations (1984) Biochem Pharmacol, 33, pp. 1525-1530; Lefkowitz, D.L., Gelderman, M.P., Fuhrmann, S.R., Neutrophilic lysozyme-macrophage interactions perpetuate chronic inflammation associated with experimental arthritis (1999) Clin Immunol, 91, pp. 145-155; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-Ciocalteau reagent (1951) J Biol Chem, 193, pp. 438-448; Meister, A., Selective modification of glutathione metabolism (1983) Science, 220, pp. 472-477; Moran, D., De Buitrago, J.M., Fernandez, E., Galan, A.I., Munoz, M.E., Jimenez, R., Inhibition of biliary glutathione secretion by cyclosporine A in the rat: Possible mechanisms and role in the cholestasis induced by the drug (1998) J Hepatol, 29, pp. 68-77; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-355; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., The structure and stereochemistry of gangetin. A new pterocarpan from Desmodium gangeticum (1971) J Chem Soc, 100, pp. 2420-2422; Rathi, A., ChV, R., Ravishankar, B., De, S., Mehrotra, S., Antiinflammatory and anti-nociceptive activity of water decoction of Desmodium gangeticum (2004) J Ethnopharmacol, 95, pp. 259-263; Rotruck, J.T., Poe, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium-biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Sudina, G.F., Mirzoeva, O.K., Pushkareva, M.A., Korshunova, G.A., Sumbatyan, N.V., Varfolomeev, S.D., Caffeic acid phenethyl ester as lipoxygenase inhibitor with antioxidant properties (1993) FEBS Lett, 329, pp. 21-24; Turner, R.A., (1965) Screening Methods in Pharmacology, 1, pp. 26-17. , Academic Press: New York; Valentao, P., Fernandes, E., Canvalho, E., Andrade, P.B., Seabra, R.M., Bastos, M.L., Antioxidant activity of Hypericum androsaenium infusion scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol Pharm Bull, 25, pp. 1324-1327; Winter, C.A., Risley, E.A., Nuss, G.W., Carrageenan induced oedema in hind paw of the rat as assay for anti-inflammatory drugs (1962) Exp Biol Med, 111, pp. 544-547; Yoshikawa, T., Naito, Y., The role of neutrophils and inflammation in gastric mucosal injury (2000) Free Radic Res, 33, pp. 785-794",
year = "2007",
doi = "10.1002/ptr.2199",
language = "English",
volume = "21",
pages = "975--979",
journal = "Phytotherapy Research",
issn = "0951-418X",
publisher = "John Wiley and Sons Ltd",
number = "10",

}

Govindarajan, R, Vijayakumar, M, Rao, CV, Shirwaikar, A, Kumar, S, Rawat, AKS & Pushpangadan, P 2007, 'Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats', Phytotherapy Research, vol. 21, no. 10, pp. 975-979. https://doi.org/10.1002/ptr.2199

Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats. / Govindarajan, R.; Vijayakumar, M.; Rao, Ch.V.; Shirwaikar, A.; Kumar, S.; Rawat, A.K.S.; Pushpangadan, P.

In: Phytotherapy Research, Vol. 21, No. 10, 2007, p. 975-979.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiinflammatory and antioxidant activities of Desmodium gangeticum fractions in carrageenan-induced inflamed rats

AU - Govindarajan, R.

AU - Vijayakumar, M.

AU - Rao, Ch.V.

AU - Shirwaikar, A.

AU - Kumar, S.

AU - Rawat, A.K.S.

AU - Pushpangadan, P.

N1 - Cited By :22 Export Date: 10 November 2017 CODEN: PHYRE Correspondence Address: Govindarajan, R.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Rana Pratap Marg 226 001, India; email: govind108@yahoo.com Chemicals/CAS: caffeic acid, 27323-69-9, 331-39-5; carrageenan, 9000-07-1, 9049-05-2, 9061-82-9, 9064-57-7; catalase, 9001-05-2; chlorogenic acid, 327-97-9; ferrous sulfate, 10028-21-4, 10124-49-9, 13463-43-9, 7720-78-7, 7782-63-0; glutathione peroxidase, 9013-66-5; indometacin, 53-86-1, 74252-25-8, 7681-54-1; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Anti-Inflammatory Agents; Antioxidants; Carrageenan, 9000-07-1; Catalase, 1.11.1.6; Desmodium gangeticum extract; Flavonoids; Glutathione Peroxidase, 1.11.1.9; Indomethacin, 53-86-1; Plant Extracts; Superoxide Dismutase, 1.15.1.1 Manufacturers: Sigma, United States References: Al-Qirim, T.M., Shahwan, M., Zaidi, K.R., Uddin, Q., Banu, N., Effect of khat, its constituents and restraint stress in free radical metabolism of rats (2002) J Ethnopharmacol, 83, pp. 245-250; Carlo, G.D., Mascolo, N., Izzo, A.A., Capasso, F., Flavonoids: Old and new aspects of a class of natural therapeutic drugs (1999) Life Sci, 65, pp. 337-353; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, pp. 33-35. , Council of Scientific and Industrial Research: New Delhi; Cohen, G., Dembiec, D., Marcus, J., Measurement of catalase activity in tissue extracts (1970) Anal Biochem, 34, pp. 30-38; Foley, S., Navaratnam, S., David, J.M., Edward, J.L., George, T., Rice-Evans, C.A., Singlet oxygen quenching and the redox properties of hydroxycinnamic acids (1999) Free Radic Biol Med, 26, pp. 1206-1208; Ghosal, S., Bhattacharya, S.K., Desmodium alkaloids II, chemical and pharmacological evaluation of D. gangeticum (1972) Planta Med, 22, pp. 434-440; Ghosh, D., Anandkumar, A., Anti-inflammatory and analgesic activities of gangetin - A pterocarpenoid from Desmodium gangeticum (1981) Indian J Pharmacol, 15, pp. 391-402; Govindarajan, R., Rastogi, S., Vijayakumar, M., Studies on antioxidant activities of Desmodium gangeticum (2003) Biol Pharm Bull, 26, pp. 1424-1427; Govindarajan, R., Vijayakumar, M., Pushpangadan, P., Antioxidant approach to disease management and the role of Rasayana herbs of Ayurveda (2005) J Ethnopharmacol, 99, pp. 165-178; Halliwell, B., Gutteridge, J.M.C., Cross, C.E., Free radicals, antioxidants and human diseases: Where are we now? (1992) J Lab Clin Med, 119, pp. 598-620; Haraguchi, H., Saitao, T., Ishikawa, H., Antiperoxidative components in Thymus vulgaris (1996) Planta Med, 62, pp. 217-221; Harman, D., The aging process: Major risk factor for disease and death (1991) Proc Natl Acad Sci, 88, pp. 5360-5370; Hogg, N., Free radicals in disease (1998) Semin Reprod Endocrinol, 16, pp. 241-248; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Indian J Biochem Biophys, 21, pp. 130-132; Landolfi, R., Mower, R.L., Steiner, M., Modification of platelet function and arachidonic acid metabolism by bioflavonoids: Structure-activity relations (1984) Biochem Pharmacol, 33, pp. 1525-1530; Lefkowitz, D.L., Gelderman, M.P., Fuhrmann, S.R., Neutrophilic lysozyme-macrophage interactions perpetuate chronic inflammation associated with experimental arthritis (1999) Clin Immunol, 91, pp. 145-155; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-Ciocalteau reagent (1951) J Biol Chem, 193, pp. 438-448; Meister, A., Selective modification of glutathione metabolism (1983) Science, 220, pp. 472-477; Moran, D., De Buitrago, J.M., Fernandez, E., Galan, A.I., Munoz, M.E., Jimenez, R., Inhibition of biliary glutathione secretion by cyclosporine A in the rat: Possible mechanisms and role in the cholestasis induced by the drug (1998) J Hepatol, 29, pp. 68-77; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, pp. 351-355; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., The structure and stereochemistry of gangetin. A new pterocarpan from Desmodium gangeticum (1971) J Chem Soc, 100, pp. 2420-2422; Rathi, A., ChV, R., Ravishankar, B., De, S., Mehrotra, S., Antiinflammatory and anti-nociceptive activity of water decoction of Desmodium gangeticum (2004) J Ethnopharmacol, 95, pp. 259-263; Rotruck, J.T., Poe, A.L., Ganther, H.E., Swanson, A.B., Hafeman, D.G., Hoekstra, W.G., Selenium-biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Sudina, G.F., Mirzoeva, O.K., Pushkareva, M.A., Korshunova, G.A., Sumbatyan, N.V., Varfolomeev, S.D., Caffeic acid phenethyl ester as lipoxygenase inhibitor with antioxidant properties (1993) FEBS Lett, 329, pp. 21-24; Turner, R.A., (1965) Screening Methods in Pharmacology, 1, pp. 26-17. , Academic Press: New York; Valentao, P., Fernandes, E., Canvalho, E., Andrade, P.B., Seabra, R.M., Bastos, M.L., Antioxidant activity of Hypericum androsaenium infusion scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol Pharm Bull, 25, pp. 1324-1327; Winter, C.A., Risley, E.A., Nuss, G.W., Carrageenan induced oedema in hind paw of the rat as assay for anti-inflammatory drugs (1962) Exp Biol Med, 111, pp. 544-547; Yoshikawa, T., Naito, Y., The role of neutrophils and inflammation in gastric mucosal injury (2000) Free Radic Res, 33, pp. 785-794

PY - 2007

Y1 - 2007

N2 - Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan-induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization. Copyright © 2007 John Wiley & Sons, Ltd.

AB - Flavonoid and alkaloid fractions of Desmodium gangeticum were evaluated for antiinflammatory and antioxidant activities in carrageenan-induced inflamed rats with the aim of studying the promising fraction for inhibitory action on ferrous sulphate induced lipid peroxidation, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPX) and total reduced glutathione in liver and spleen homogenates of inflamed rats. The results showed that the flavonoid fraction of D. gangeticum possesses potent antioxidant activity compared with the alkaloid fraction and also with respect to the standard drug indomethacin, in terms of augmentation of the liver and spleen SOD, CAT and GPX activities, concomitant with a reduction in lipid peroxidation (TBARS). In addition, the quantification of caffeic and chlorogenic acid in the flavonoid fraction has also been carried out using HPLC, which can be utilized as a marker compound in the standardization. Copyright © 2007 John Wiley & Sons, Ltd.

U2 - 10.1002/ptr.2199

DO - 10.1002/ptr.2199

M3 - Article

VL - 21

SP - 975

EP - 979

JO - Phytotherapy Research

JF - Phytotherapy Research

SN - 0951-418X

IS - 10

ER -