Antiinflammatory and antiulcer activities of phytic acid in rats

M. Sudheer Kumar, B. Sridhar Reddy, S. Kiran Babu, P.M. Bhilegaonkar, A. Shirwaikar, M.K. Unnikrishnan

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

Maximum antiinflammatory activity of phytic acid (PA) was seen at an oral dose of 150 mg/kg in the carrageenan induced rat paw edema model. Although PA showed ability to prevent denaturation of proteins, it showed less antiinflammatory activity than ibuprofen. Ability of PA to bring down thermal denaturation of proteins might be a contributing factor in the mechanism of action against inflammation. PA, at all the doses tested, showed significant protection from ulcers induced by ibuprofen, ethanol and cold stress, with a maximum activity at 150 mg/kg. There was a significant increase in gastric tissue malondialdehyde levels in ethanol treated rats but these levels decreased following PA pretreatment, Moreover, pretreatment with PA significantly inhibited various effects of ethanol on gastric mucosa, such as, reduction in the concentration of nonprotein sulfhydryl groups, necrosis, erosions, congestion and hemorrhage. These results suggested that gastro-protective effect of PA could be mediated by its antioxidant activity and cytoprotection of gastric mucosa.
Original languageEnglish
Pages (from-to)179-185
Number of pages7
JournalIndian Journal of Experimental Biology
Volume42
Issue number2
Publication statusPublished - 2004

Fingerprint

Phytic Acid
Anti-Inflammatory Agents
Protein Denaturation
Ethanol
Ibuprofen
Gastric Mucosa
Cytoprotection
Carrageenan
Malondialdehyde
Ulcer
Edema
Stomach
Necrosis
Antioxidants
Hot Temperature
Hemorrhage
Inflammation

Cite this

Sudheer Kumar, M., Sridhar Reddy, B., Kiran Babu, S., Bhilegaonkar, P. M., Shirwaikar, A., & Unnikrishnan, M. K. (2004). Antiinflammatory and antiulcer activities of phytic acid in rats. Indian Journal of Experimental Biology, 42(2), 179-185.
Sudheer Kumar, M. ; Sridhar Reddy, B. ; Kiran Babu, S. ; Bhilegaonkar, P.M. ; Shirwaikar, A. ; Unnikrishnan, M.K. / Antiinflammatory and antiulcer activities of phytic acid in rats. In: Indian Journal of Experimental Biology. 2004 ; Vol. 42, No. 2. pp. 179-185.
@article{affb3726156a47099e30af4036bf5bbc,
title = "Antiinflammatory and antiulcer activities of phytic acid in rats",
abstract = "Maximum antiinflammatory activity of phytic acid (PA) was seen at an oral dose of 150 mg/kg in the carrageenan induced rat paw edema model. Although PA showed ability to prevent denaturation of proteins, it showed less antiinflammatory activity than ibuprofen. Ability of PA to bring down thermal denaturation of proteins might be a contributing factor in the mechanism of action against inflammation. PA, at all the doses tested, showed significant protection from ulcers induced by ibuprofen, ethanol and cold stress, with a maximum activity at 150 mg/kg. There was a significant increase in gastric tissue malondialdehyde levels in ethanol treated rats but these levels decreased following PA pretreatment, Moreover, pretreatment with PA significantly inhibited various effects of ethanol on gastric mucosa, such as, reduction in the concentration of nonprotein sulfhydryl groups, necrosis, erosions, congestion and hemorrhage. These results suggested that gastro-protective effect of PA could be mediated by its antioxidant activity and cytoprotection of gastric mucosa.",
author = "{Sudheer Kumar}, M. and {Sridhar Reddy}, B. and {Kiran Babu}, S. and P.M. Bhilegaonkar and A. Shirwaikar and M.K. Unnikrishnan",
note = "Cited By :18 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, College of Pharmaceutical Sciences, Manipal 576 119, India; email: cheruvaloor@yahoo.com Chemicals/CAS: alcohol, 64-17-5; famotidine, 76824-35-6; ibuprofen, 15687-27-1; malonaldehyde, 542-78-9; phytic acid, 83-86-3; thiol derivative, 13940-21-1; Anti-Inflammatory Agents; Anti-Ulcer Agents; Ethanol, 64-17-5; Famotidine, 76824-35-6; Ibuprofen, 15687-27-1; Malondialdehyde, 542-78-9; Phytic Acid, 83-86-3; Plant Extracts; Sulfhydryl Compounds References: Graf, E., Eaton, J.W., Suppression of colonic cancer by dietary phytic acid (1993) Nutr Cancer, 19, p. 11; Shamsuddin, A.M., Inositol phosphates have novel anticancer function (1995) J Nutr, 125, pp. 725S; Graf, E., Eaton, J.W., Antioxidant functions of phytic acid (1990) Free Radical Biol Med, 8, p. 61; Hawkins, P.T., Poyner, D.R., Jackson, T.R., Letcher, A.J., Lander, D.A., Irvine, R.F., Inhibition of iron-catalyzed hydroxyl radical formation by inositol polyphosphates: A possible physiological function for myo-inositol hexakisphosphate (1993) Biochem J, 294, p. 929; Phillippy, B.Q., Ullah, A.H., Ehrlich, K.C., Purification and some properties of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from immature soyabean seeds (1994) J Biol Chem, 269, p. 28393; Timerman, A.P., Mayrleitner, M.M., Lukas, T.J., Chadwick, C.C., Watterson, D.M., Schlindler, H., Fleischer, S., Inositol polyphosphate receptor and clathrin assembly protein AP-2 are related proteins that form potassium-selective ion channels in planner lipid bilayers (1992) Proc Natl Acad Sci USA, 89, p. 8976; Shamsuddin, A.M., Yang, G.Y., Vucenik, I., Novel anti-cancer functions of IP6: Growth inhibition and differentiation of human mammary cancer cell lines in vitro (1996) Anticancer Res, 16, p. 3287; Graf, E., Katherine, L.E., John, W.E., Phytic acid a natural antioxidant (1987) J Biol Chem, 262, p. 11647; Mizushima, Y., Kobayashi, M., Interaction of antiinflammatory drugs with serum proteins, especially with some biologically active proteins (1967) J Pharm Pharmacol, 20, p. 169; Elias, G., Rao, M.N.A., Inhibition of albumin denaturation and antiinflammatory activity of dehydrozingerone and its analogs (1988) Indian J Exp Biol, 26, p. 540; Rao, C.M., Ramesh, K.V., Bairy, K.L., Kulkarni, D.R., Zinc complexes of NSAIDs abolish gastric ulceration prospensity of parent drugs (1990) Indian Drugs, 28, p. 64; Shah, A.H., Khan, Z.A., Baig, M.Z.A., Qureshi, S., Al-Bekairi, A.M., Gastroprotective effect of pretreatment with Zizyphus saliva fruits against toxic damage in rats (1997) Fitoterapia, 3, p. 226; Takeuchi, K., Ueshima, K., Ohuchi, T., Okabe, S., The role of capsiacin sensitive sensory neurons in healing of HCl-induced mucosal lesions in rats (1994) Gastroenterolgy, 104, p. 1524; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, p. 351; Sedlak, J., Lindsay, R.H., Estimation of total protein and non protein sulfhydryl groups in tissue with Ellam's reagent (1968) Anal Biochem, 25, p. 192; Sies, H., Antioxidants, Redox-regulated transcription factors and inflammation (1997) Antioxidants in Disease Mechanism and Therapy, p. 403. , edited by P G Winyard & D R Blake, (London: Academic Press, California, USA); Robert, A., Nezamis, J.E., Lancaster, C., Hanchar, A.J., Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury (1979) Gastroenterology, 77, p. 433; Naito, Y., Yoshikawa, T., Matsuyama, K., Yagi, N., Aral, M., Nakamura, S., Yoshida, N., Konda, M., Effect of oxygen radical scavengers on the quality of gastric ulcer healing in rats (1995) J Clin Gastroenterol, 21, pp. S82; Desai, J.K., Goyal, R.K., Parmar, N.S., Pathogenesis of peptic ulcer disease and current trends in therapy (1997) Indian J Physio Pharmacol, 41, p. 3; Szelenyi, I., Brune, K., Possible role of free radicals in ethanol-induced gastric mucosal damage in rats (1988) Dig Dis Sci, 33, p. 865; Szabo, S., Trier, J.S., Frankel, P.W., Sulfhydryl compounds may mediate gastric cytoprotection (1981) Science, 214, p. 200; Hua, X.Y., Dahlen, S.E., Lundberg, J.M., Hammerstrom, S., Hedqvist, P., Leckotrienes C4, D4, E4 cause wide spread and extensive plasma extravasation in the guineapig (1985) Naunyn-Schmiedeberg's Arch Pharmacol, 330, p. 136; Bandyopadhyay, U., Das, D., Bandyopadhyay, D., Bhattacharjee, M., Banerjee, R.K., Role of reactive oxygen species in mercaptomethylimidazole-induced gastric acid secretion and stress-induced gastric ulceration (1999) Curr Sci, 76, p. 55; Holley, A.E., Cheeseman, K.H., Measuring free radical reaction in vivo (1993) Br Med Bull, 49, p. 407; Al-Harabi, M.M., Qureshi, S., Raza, M., Ahmed, M.M., Afzal, M., Shah, A.H., Evaluation of caralluma tuberculata pretreatment for the protection of rat gastric mucosa against toxic damage (1994) Toxicol Applied Pharmacol, 128, p. 1; Al-Bekairi, A.M., Qureshi, S., Ahmed, M.M., Afzal, M., Shah, A.H., A study of uric acid pretreatment for the protection of gastric mucosa against toxic damage (1992) Food Chem Toxicol, 30, p. 525; Phillipy, B.Q., Graf, E., Antioxidant functions of inositol 1,2,3-triphosphate and inositol 1,2,3,6-tetrakisphosphate (1997) Free Radical Biol Med, 22, p. 939; Albin, J., Shalev, O., Karmeli, F., Rachmilewitz, D., Deferiprone, an oral iron chelator, ameliorates experimental colitis and gastric ulceration in rats (1999) Inflamm Bowel Dis, 5, p. 253; Singh, A., Singh, S.P., Bamezai, R., Modulatory influence of arecoline on phytic acid altered hepatic biotransformation system enzymes, sulfhydryl content and lipid peroxidation in a murine system (1997) Cancer Lett, 117, p. 1",
year = "2004",
language = "English",
volume = "42",
pages = "179--185",
journal = "Journal of scientific & industrial research. C. Biological sciences",
issn = "0019-5189",
publisher = "National Institute of Science Communication",
number = "2",

}

Sudheer Kumar, M, Sridhar Reddy, B, Kiran Babu, S, Bhilegaonkar, PM, Shirwaikar, A & Unnikrishnan, MK 2004, 'Antiinflammatory and antiulcer activities of phytic acid in rats', Indian Journal of Experimental Biology, vol. 42, no. 2, pp. 179-185.

Antiinflammatory and antiulcer activities of phytic acid in rats. / Sudheer Kumar, M.; Sridhar Reddy, B.; Kiran Babu, S.; Bhilegaonkar, P.M.; Shirwaikar, A.; Unnikrishnan, M.K.

In: Indian Journal of Experimental Biology, Vol. 42, No. 2, 2004, p. 179-185.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiinflammatory and antiulcer activities of phytic acid in rats

AU - Sudheer Kumar, M.

AU - Sridhar Reddy, B.

AU - Kiran Babu, S.

AU - Bhilegaonkar, P.M.

AU - Shirwaikar, A.

AU - Unnikrishnan, M.K.

N1 - Cited By :18 Export Date: 10 November 2017 CODEN: IJEBA Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, College of Pharmaceutical Sciences, Manipal 576 119, India; email: cheruvaloor@yahoo.com Chemicals/CAS: alcohol, 64-17-5; famotidine, 76824-35-6; ibuprofen, 15687-27-1; malonaldehyde, 542-78-9; phytic acid, 83-86-3; thiol derivative, 13940-21-1; Anti-Inflammatory Agents; Anti-Ulcer Agents; Ethanol, 64-17-5; Famotidine, 76824-35-6; Ibuprofen, 15687-27-1; Malondialdehyde, 542-78-9; Phytic Acid, 83-86-3; Plant Extracts; Sulfhydryl Compounds References: Graf, E., Eaton, J.W., Suppression of colonic cancer by dietary phytic acid (1993) Nutr Cancer, 19, p. 11; Shamsuddin, A.M., Inositol phosphates have novel anticancer function (1995) J Nutr, 125, pp. 725S; Graf, E., Eaton, J.W., Antioxidant functions of phytic acid (1990) Free Radical Biol Med, 8, p. 61; Hawkins, P.T., Poyner, D.R., Jackson, T.R., Letcher, A.J., Lander, D.A., Irvine, R.F., Inhibition of iron-catalyzed hydroxyl radical formation by inositol polyphosphates: A possible physiological function for myo-inositol hexakisphosphate (1993) Biochem J, 294, p. 929; Phillippy, B.Q., Ullah, A.H., Ehrlich, K.C., Purification and some properties of inositol 1,3,4,5,6-pentakisphosphate 2-kinase from immature soyabean seeds (1994) J Biol Chem, 269, p. 28393; Timerman, A.P., Mayrleitner, M.M., Lukas, T.J., Chadwick, C.C., Watterson, D.M., Schlindler, H., Fleischer, S., Inositol polyphosphate receptor and clathrin assembly protein AP-2 are related proteins that form potassium-selective ion channels in planner lipid bilayers (1992) Proc Natl Acad Sci USA, 89, p. 8976; Shamsuddin, A.M., Yang, G.Y., Vucenik, I., Novel anti-cancer functions of IP6: Growth inhibition and differentiation of human mammary cancer cell lines in vitro (1996) Anticancer Res, 16, p. 3287; Graf, E., Katherine, L.E., John, W.E., Phytic acid a natural antioxidant (1987) J Biol Chem, 262, p. 11647; Mizushima, Y., Kobayashi, M., Interaction of antiinflammatory drugs with serum proteins, especially with some biologically active proteins (1967) J Pharm Pharmacol, 20, p. 169; Elias, G., Rao, M.N.A., Inhibition of albumin denaturation and antiinflammatory activity of dehydrozingerone and its analogs (1988) Indian J Exp Biol, 26, p. 540; Rao, C.M., Ramesh, K.V., Bairy, K.L., Kulkarni, D.R., Zinc complexes of NSAIDs abolish gastric ulceration prospensity of parent drugs (1990) Indian Drugs, 28, p. 64; Shah, A.H., Khan, Z.A., Baig, M.Z.A., Qureshi, S., Al-Bekairi, A.M., Gastroprotective effect of pretreatment with Zizyphus saliva fruits against toxic damage in rats (1997) Fitoterapia, 3, p. 226; Takeuchi, K., Ueshima, K., Ohuchi, T., Okabe, S., The role of capsiacin sensitive sensory neurons in healing of HCl-induced mucosal lesions in rats (1994) Gastroenterolgy, 104, p. 1524; Ohkawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxidation in animal tissues by thiobarbituric acid reaction (1979) Anal Biochem, 95, p. 351; Sedlak, J., Lindsay, R.H., Estimation of total protein and non protein sulfhydryl groups in tissue with Ellam's reagent (1968) Anal Biochem, 25, p. 192; Sies, H., Antioxidants, Redox-regulated transcription factors and inflammation (1997) Antioxidants in Disease Mechanism and Therapy, p. 403. , edited by P G Winyard & D R Blake, (London: Academic Press, California, USA); Robert, A., Nezamis, J.E., Lancaster, C., Hanchar, A.J., Cytoprotection by prostaglandins in rats. Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury (1979) Gastroenterology, 77, p. 433; Naito, Y., Yoshikawa, T., Matsuyama, K., Yagi, N., Aral, M., Nakamura, S., Yoshida, N., Konda, M., Effect of oxygen radical scavengers on the quality of gastric ulcer healing in rats (1995) J Clin Gastroenterol, 21, pp. S82; Desai, J.K., Goyal, R.K., Parmar, N.S., Pathogenesis of peptic ulcer disease and current trends in therapy (1997) Indian J Physio Pharmacol, 41, p. 3; Szelenyi, I., Brune, K., Possible role of free radicals in ethanol-induced gastric mucosal damage in rats (1988) Dig Dis Sci, 33, p. 865; Szabo, S., Trier, J.S., Frankel, P.W., Sulfhydryl compounds may mediate gastric cytoprotection (1981) Science, 214, p. 200; Hua, X.Y., Dahlen, S.E., Lundberg, J.M., Hammerstrom, S., Hedqvist, P., Leckotrienes C4, D4, E4 cause wide spread and extensive plasma extravasation in the guineapig (1985) Naunyn-Schmiedeberg's Arch Pharmacol, 330, p. 136; Bandyopadhyay, U., Das, D., Bandyopadhyay, D., Bhattacharjee, M., Banerjee, R.K., Role of reactive oxygen species in mercaptomethylimidazole-induced gastric acid secretion and stress-induced gastric ulceration (1999) Curr Sci, 76, p. 55; Holley, A.E., Cheeseman, K.H., Measuring free radical reaction in vivo (1993) Br Med Bull, 49, p. 407; Al-Harabi, M.M., Qureshi, S., Raza, M., Ahmed, M.M., Afzal, M., Shah, A.H., Evaluation of caralluma tuberculata pretreatment for the protection of rat gastric mucosa against toxic damage (1994) Toxicol Applied Pharmacol, 128, p. 1; Al-Bekairi, A.M., Qureshi, S., Ahmed, M.M., Afzal, M., Shah, A.H., A study of uric acid pretreatment for the protection of gastric mucosa against toxic damage (1992) Food Chem Toxicol, 30, p. 525; Phillipy, B.Q., Graf, E., Antioxidant functions of inositol 1,2,3-triphosphate and inositol 1,2,3,6-tetrakisphosphate (1997) Free Radical Biol Med, 22, p. 939; Albin, J., Shalev, O., Karmeli, F., Rachmilewitz, D., Deferiprone, an oral iron chelator, ameliorates experimental colitis and gastric ulceration in rats (1999) Inflamm Bowel Dis, 5, p. 253; Singh, A., Singh, S.P., Bamezai, R., Modulatory influence of arecoline on phytic acid altered hepatic biotransformation system enzymes, sulfhydryl content and lipid peroxidation in a murine system (1997) Cancer Lett, 117, p. 1

PY - 2004

Y1 - 2004

N2 - Maximum antiinflammatory activity of phytic acid (PA) was seen at an oral dose of 150 mg/kg in the carrageenan induced rat paw edema model. Although PA showed ability to prevent denaturation of proteins, it showed less antiinflammatory activity than ibuprofen. Ability of PA to bring down thermal denaturation of proteins might be a contributing factor in the mechanism of action against inflammation. PA, at all the doses tested, showed significant protection from ulcers induced by ibuprofen, ethanol and cold stress, with a maximum activity at 150 mg/kg. There was a significant increase in gastric tissue malondialdehyde levels in ethanol treated rats but these levels decreased following PA pretreatment, Moreover, pretreatment with PA significantly inhibited various effects of ethanol on gastric mucosa, such as, reduction in the concentration of nonprotein sulfhydryl groups, necrosis, erosions, congestion and hemorrhage. These results suggested that gastro-protective effect of PA could be mediated by its antioxidant activity and cytoprotection of gastric mucosa.

AB - Maximum antiinflammatory activity of phytic acid (PA) was seen at an oral dose of 150 mg/kg in the carrageenan induced rat paw edema model. Although PA showed ability to prevent denaturation of proteins, it showed less antiinflammatory activity than ibuprofen. Ability of PA to bring down thermal denaturation of proteins might be a contributing factor in the mechanism of action against inflammation. PA, at all the doses tested, showed significant protection from ulcers induced by ibuprofen, ethanol and cold stress, with a maximum activity at 150 mg/kg. There was a significant increase in gastric tissue malondialdehyde levels in ethanol treated rats but these levels decreased following PA pretreatment, Moreover, pretreatment with PA significantly inhibited various effects of ethanol on gastric mucosa, such as, reduction in the concentration of nonprotein sulfhydryl groups, necrosis, erosions, congestion and hemorrhage. These results suggested that gastro-protective effect of PA could be mediated by its antioxidant activity and cytoprotection of gastric mucosa.

M3 - Article

VL - 42

SP - 179

EP - 185

JO - Journal of scientific & industrial research. C. Biological sciences

JF - Journal of scientific & industrial research. C. Biological sciences

SN - 0019-5189

IS - 2

ER -

Sudheer Kumar M, Sridhar Reddy B, Kiran Babu S, Bhilegaonkar PM, Shirwaikar A, Unnikrishnan MK. Antiinflammatory and antiulcer activities of phytic acid in rats. Indian Journal of Experimental Biology. 2004;42(2):179-185.