Antimicrobial, anticancer and cytotoxic activities of acetone extract fraction from stem bark of Thespesia populnea (Linn.)

E. Jeyaseeli Florance, D. Dhayabaran, K. Nandakumar

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Abstract

The fraction from the acetone extract of stem bark of Thespesia populnea (Linn.) was investigated to explore the antimicrobial, anticancer and cytotoxic properties. The screening of antimicrobial activity of the fraction was carried out by cup plate method against four standard bacterial [Staphylococcus aureus (MTCC 96), Micrococcus luteus (MTCC 1541), Klebsiella pneumonia (MTCC 109) and Escherichia coli (MTCC 118)] and three fungal strains [Candida albicans (MTCC 183), C. parapsilosis (MTCC 2509) and C. tropicalis (MTCC 184)]. The anticancerous activity against HEp2 cell line and cytotoxicity assay against HEL cell line were carried out by standard protocols. The results indicated that the fraction possess comparatively higher antibacterial activity than antifungal activity. While the fraction inhibited all the other three bacteria at the concentration of ≥ 500 μg/ml, it was ineffective against K. pneumonia. A moderate antifungal activity against all the Candidal species at the concentration of ≥ 750 μg/ml was recorded. The fraction exhibited satisfactory anticancerous activity against HEp2 cell line with the concentration of 5 mg/ml (14.6% of cell viability). The cytotoxic activity of the fraction on normal cell line by MTT assay indicated that a concentration of 5 mg/ml was required to cause 50% (IC50) of inhibition of HEL cell line. Thus, the findings of the present study vividly present the potential of acetone extract fraction from stem bark of T. populnea, to be a promising antimicrobial and anticancerous with low cytotoxicity on normal cells.

Original languageEnglish
Pages (from-to)1722-1728
Number of pages7
JournalResearch Journal of Pharmaceutical, Biological and Chemical Sciences
Volume7
Issue number3
Publication statusPublished - 2016

Fingerprint

Acetone
Cells
Cell Line
Klebsiella pneumoniae
Cytotoxicity
Assays
Micrococcus luteus
Candida albicans
Candida
Inhibitory Concentration 50
Staphylococcus aureus
Cell Survival
Escherichia coli
Bacteria
Screening

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

Cite this

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title = "Antimicrobial, anticancer and cytotoxic activities of acetone extract fraction from stem bark of Thespesia populnea (Linn.)",
abstract = "The fraction from the acetone extract of stem bark of Thespesia populnea (Linn.) was investigated to explore the antimicrobial, anticancer and cytotoxic properties. The screening of antimicrobial activity of the fraction was carried out by cup plate method against four standard bacterial [Staphylococcus aureus (MTCC 96), Micrococcus luteus (MTCC 1541), Klebsiella pneumonia (MTCC 109) and Escherichia coli (MTCC 118)] and three fungal strains [Candida albicans (MTCC 183), C. parapsilosis (MTCC 2509) and C. tropicalis (MTCC 184)]. The anticancerous activity against HEp2 cell line and cytotoxicity assay against HEL cell line were carried out by standard protocols. The results indicated that the fraction possess comparatively higher antibacterial activity than antifungal activity. While the fraction inhibited all the other three bacteria at the concentration of ≥ 500 μg/ml, it was ineffective against K. pneumonia. A moderate antifungal activity against all the Candidal species at the concentration of ≥ 750 μg/ml was recorded. The fraction exhibited satisfactory anticancerous activity against HEp2 cell line with the concentration of 5 mg/ml (14.6{\%} of cell viability). The cytotoxic activity of the fraction on normal cell line by MTT assay indicated that a concentration of 5 mg/ml was required to cause 50{\%} (IC50) of inhibition of HEL cell line. Thus, the findings of the present study vividly present the potential of acetone extract fraction from stem bark of T. populnea, to be a promising antimicrobial and anticancerous with low cytotoxicity on normal cells.",
author = "{Jeyaseeli Florance}, E. and D. Dhayabaran and K. Nandakumar",
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T1 - Antimicrobial, anticancer and cytotoxic activities of acetone extract fraction from stem bark of Thespesia populnea (Linn.)

AU - Jeyaseeli Florance, E.

AU - Dhayabaran, D.

AU - Nandakumar, K.

PY - 2016

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N2 - The fraction from the acetone extract of stem bark of Thespesia populnea (Linn.) was investigated to explore the antimicrobial, anticancer and cytotoxic properties. The screening of antimicrobial activity of the fraction was carried out by cup plate method against four standard bacterial [Staphylococcus aureus (MTCC 96), Micrococcus luteus (MTCC 1541), Klebsiella pneumonia (MTCC 109) and Escherichia coli (MTCC 118)] and three fungal strains [Candida albicans (MTCC 183), C. parapsilosis (MTCC 2509) and C. tropicalis (MTCC 184)]. The anticancerous activity against HEp2 cell line and cytotoxicity assay against HEL cell line were carried out by standard protocols. The results indicated that the fraction possess comparatively higher antibacterial activity than antifungal activity. While the fraction inhibited all the other three bacteria at the concentration of ≥ 500 μg/ml, it was ineffective against K. pneumonia. A moderate antifungal activity against all the Candidal species at the concentration of ≥ 750 μg/ml was recorded. The fraction exhibited satisfactory anticancerous activity against HEp2 cell line with the concentration of 5 mg/ml (14.6% of cell viability). The cytotoxic activity of the fraction on normal cell line by MTT assay indicated that a concentration of 5 mg/ml was required to cause 50% (IC50) of inhibition of HEL cell line. Thus, the findings of the present study vividly present the potential of acetone extract fraction from stem bark of T. populnea, to be a promising antimicrobial and anticancerous with low cytotoxicity on normal cells.

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