Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc

M. Govindappa, S. Naga Sravya, M. N. Poojashri, T. S. Sadananda, C. P. Chandrappa, Gustavo Santoyo, P. Sharanappa, N. V. Anil Kumar

Research output: Contribution to journalArticle

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Abstract

The aim of the study was to evaluate antimicrobial, antioxidant and anti-inflammatory activity of dry and fresh parts of leaf, stem and flower from the water extract of Wedelia trilobata. The antimicrobial activity of water extracts of fresh and dry parts against 9 different strains of bacteria and 11 different species of fungi were determined using standard method (paper disc method). The fresh parts water extracts showed that, leaf and flower extracts were most potent inhibiting all isolates of with different zones of inhibition but did not inhibited the growth of fungi tested. All the extracts have only moderately inhibited the all fungi. The minimum microbial concentration (MMC) of the active extract was observed from fresh part extracts of leaf, flower and stem ranged from 0.4 to 5.0 mg/ml for the sensitive bacteria. In case of fungi, the minimum inhibitory concentration (MIC) of the active extracts ranged from 2.4 to 6.0 mg/ml. Together, these data suggest that the W. trilobata fresh parts extracts analyzed are potential antimicrobial candidates with a broad range of activity. Phytochemical screening of extracts showed the presence of tannins, cardiac glycosides, flavonoids, terpenoids, phenols, saponins and coumarins. Leaf and flower water have showed highest total phenolic content. In 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) method, the leaf and flower had showed free radical inhibition of 86, 83 and 1623.21, 1611.26, respectively and they also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and red blood cells membrane stabilization with 89.61 and 86.81 and 78.82, 76.65 g/ml, respectively. Proteinase activity was also significantly inhibited by the leaf (83.91 g/ml) and flower (81.17 g/ml). From the result, it is concluded that phytochemicals present in the W. trilobata extract may be responsible and can be used as antimicrobial, antioxidant and anti-inflammatory agent.

Original languageEnglish
Pages (from-to)5718-5729
Number of pages12
JournalJournal of Medicinal Plant Research
Volume5
Issue number24
Publication statusPublished - 30-10-2011

Fingerprint

Wedelia
Sphagneticola trilobata
Phytochemicals
anti-inflammatory activity
phytopharmaceuticals
Anti-Inflammatory Agents
Antioxidants
anti-infective agents
screening
antioxidants
Water
extracts
Fungi
flowers
water
Fresh Water
fungi
Bacteria
Coumarins
leaf extracts

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmaceutical Science
  • Plant Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Govindappa, M. ; Naga Sravya, S. ; Poojashri, M. N. ; Sadananda, T. S. ; Chandrappa, C. P. ; Santoyo, Gustavo ; Sharanappa, P. ; Anil Kumar, N. V. / Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc. In: Journal of Medicinal Plant Research. 2011 ; Vol. 5, No. 24. pp. 5718-5729.
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abstract = "The aim of the study was to evaluate antimicrobial, antioxidant and anti-inflammatory activity of dry and fresh parts of leaf, stem and flower from the water extract of Wedelia trilobata. The antimicrobial activity of water extracts of fresh and dry parts against 9 different strains of bacteria and 11 different species of fungi were determined using standard method (paper disc method). The fresh parts water extracts showed that, leaf and flower extracts were most potent inhibiting all isolates of with different zones of inhibition but did not inhibited the growth of fungi tested. All the extracts have only moderately inhibited the all fungi. The minimum microbial concentration (MMC) of the active extract was observed from fresh part extracts of leaf, flower and stem ranged from 0.4 to 5.0 mg/ml for the sensitive bacteria. In case of fungi, the minimum inhibitory concentration (MIC) of the active extracts ranged from 2.4 to 6.0 mg/ml. Together, these data suggest that the W. trilobata fresh parts extracts analyzed are potential antimicrobial candidates with a broad range of activity. Phytochemical screening of extracts showed the presence of tannins, cardiac glycosides, flavonoids, terpenoids, phenols, saponins and coumarins. Leaf and flower water have showed highest total phenolic content. In 2, 2-diphenyl-1-picrylhydrazyl (DPPH) and ferric ion reducing antioxidant power (FRAP) method, the leaf and flower had showed free radical inhibition of 86, 83 and 1623.21, 1611.26, respectively and they also showed in vitro anti-inflammatory activity by inhibiting the heat induced albumin denaturation and red blood cells membrane stabilization with 89.61 and 86.81 and 78.82, 76.65 g/ml, respectively. Proteinase activity was also significantly inhibited by the leaf (83.91 g/ml) and flower (81.17 g/ml). From the result, it is concluded that phytochemicals present in the W. trilobata extract may be responsible and can be used as antimicrobial, antioxidant and anti-inflammatory agent.",
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Govindappa, M, Naga Sravya, S, Poojashri, MN, Sadananda, TS, Chandrappa, CP, Santoyo, G, Sharanappa, P & Anil Kumar, NV 2011, 'Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc', Journal of Medicinal Plant Research, vol. 5, no. 24, pp. 5718-5729.

Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc. / Govindappa, M.; Naga Sravya, S.; Poojashri, M. N.; Sadananda, T. S.; Chandrappa, C. P.; Santoyo, Gustavo; Sharanappa, P.; Anil Kumar, N. V.

In: Journal of Medicinal Plant Research, Vol. 5, No. 24, 30.10.2011, p. 5718-5729.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc

AU - Govindappa, M.

AU - Naga Sravya, S.

AU - Poojashri, M. N.

AU - Sadananda, T. S.

AU - Chandrappa, C. P.

AU - Santoyo, Gustavo

AU - Sharanappa, P.

AU - Anil Kumar, N. V.

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Govindappa M, Naga Sravya S, Poojashri MN, Sadananda TS, Chandrappa CP, Santoyo G et al. Antimicrobial, antioxidant and in vitro anti-inflammatory activity and phytochemical screening of water extract of wedelia trilobata (L.) hitchc. Journal of Medicinal Plant Research. 2011 Oct 30;5(24):5718-5729.