Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats

Jyoti Shrestha, Tara Shanbhag, Smita Shenoy, Arul Amuthan, Krishnananda Prabhu, Stuti Sharma, Samik Banerjee, Sajala Kafle

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Objectives : To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats. Materials and Methods : For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16 th day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6 th to 15 th day of pregnancy. Rats were laparotomised on 19 th day. The number of implantation sites and live fetuses were observed in both horns of the uterus. Results : The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5% and 72.22%, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control. Conclusion : The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.

Original languageEnglish
Pages (from-to)306-311
Number of pages6
JournalIndian Journal of Pharmacology
Volume42
Issue number5
DOIs
Publication statusPublished - 01-10-2010

Fingerprint

Abortifacient Agents
Areca
Estrus
Cholesterol
Metestrus
Diestrus
Weights and Measures
Proestrus
Vaginal Smears
Uterus
Ovary
Fetus
Pregnancy

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Pharmacology (medical)

Cite this

Shrestha, Jyoti ; Shanbhag, Tara ; Shenoy, Smita ; Amuthan, Arul ; Prabhu, Krishnananda ; Sharma, Stuti ; Banerjee, Samik ; Kafle, Sajala. / Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats. In: Indian Journal of Pharmacology. 2010 ; Vol. 42, No. 5. pp. 306-311.
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title = "Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats",
abstract = "Objectives : To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats. Materials and Methods : For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16 th day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6 th to 15 th day of pregnancy. Rats were laparotomised on 19 th day. The number of implantation sites and live fetuses were observed in both horns of the uterus. Results : The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5{\%} and 72.22{\%}, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control. Conclusion : The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.",
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Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats. / Shrestha, Jyoti; Shanbhag, Tara; Shenoy, Smita; Amuthan, Arul; Prabhu, Krishnananda; Sharma, Stuti; Banerjee, Samik; Kafle, Sajala.

In: Indian Journal of Pharmacology, Vol. 42, No. 5, 01.10.2010, p. 306-311.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antiovulatory and abortifacient effects of Areca catechu (betel nut) in female rats

AU - Shrestha, Jyoti

AU - Shanbhag, Tara

AU - Shenoy, Smita

AU - Amuthan, Arul

AU - Prabhu, Krishnananda

AU - Sharma, Stuti

AU - Banerjee, Samik

AU - Kafle, Sajala

PY - 2010/10/1

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N2 - Objectives : To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats. Materials and Methods : For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16 th day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6 th to 15 th day of pregnancy. Rats were laparotomised on 19 th day. The number of implantation sites and live fetuses were observed in both horns of the uterus. Results : The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5% and 72.22%, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control. Conclusion : The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.

AB - Objectives : To study the antiovulatory and abortifacient effects of ethanolic extract of Areca catechu in female rats. Materials and Methods : For antiovulatory effect, ethanolic extract of A. catechu at 100 and 300 mg/kg doses was administered orally for 15 days. Vaginal smears were examined daily microscopically for estrus cycle. Rats were sacrificed on 16 th day. Ovarian weight, cholesterol estimation, and histopathological studies were done. Abortifacient activity was studied in rats at 100 and 300 mg/kg doses administered orally from 6 th to 15 th day of pregnancy. Rats were laparotomised on 19 th day. The number of implantation sites and live fetuses were observed in both horns of the uterus. Results : The extract of A. catechu showed a significant decrease in the duration of estrus at 100 mg/kg (P = 0.015) and 300 mg/kg doses (P = 0.002) as compared with control. Metestrus phase was also significantly reduced at 100 mg/kg (P = 0.024) and 300 mg/kg doses (P = 0.002). There was a significant increase in proestrus (P < 0.001) phase. However, diestrus phase was unchanged. Histopathological study of the ovaries showed mainly primordial, primary, and secondary follicles in the test groups as compared to control. There was also a significant (P = 0.002) decrease in ovarian weight and a significant (P = 0.021) increase in ovarian cholesterol level at 100 mg/kg dose. In the study to evaluate abortifacient effect, the mean percentage of abortion with 100 and 300 mg/kg doses were 75.5% and 72.22%, respectively, which was significantly (P = 0.008 and P = 0.006, respectively) increased when compared with control. Conclusion : The ethanolic extract of A. catechu at doses of 100 and 300 mg/kg has antiovulatory and abortifacient effects.

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