Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats

I.S.R. Punitha, N. Bhat, K. Rajendran, A. Shirwaikar

Research output: Contribution to journalArticle

Abstract

The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.
Original languageEnglish
Pages (from-to)155-159
Number of pages5
JournalNatural Product Sciences
Volume11
Issue number3
Publication statusPublished - 2005

Fingerprint

Niacinamide
Rats
Antioxidants
Glutathione Peroxidase
Glutathione Synthase
Ceruloplasmin
Oxidative stress
Tocopherols
Medical problems
Catalase
Type 2 Diabetes Mellitus
Peroxidase
Ascorbic Acid
Superoxide Dismutase
Oxidative Stress

Cite this

Punitha, I. S. R., Bhat, N., Rajendran, K., & Shirwaikar, A. (2005). Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats. Natural Product Sciences, 11(3), 155-159.
Punitha, I.S.R. ; Bhat, N. ; Rajendran, K. ; Shirwaikar, A. / Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats. In: Natural Product Sciences. 2005 ; Vol. 11, No. 3. pp. 155-159.
@article{e2b4c74b613c43998eff8a23016a6dfd,
title = "Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats",
abstract = "The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.",
author = "I.S.R. Punitha and N. Bhat and K. Rajendran and A. Shirwaikar",
note = "Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; ceruloplasmin, 9031-37-2; glutathione peroxidase, 9013-66-5; glutathione synthase, 9023-62-5; nicotinamide, 11032-50-1, 98-92-0; peroxidase, 9003-99-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tocopherol, 1406-66-2 References: Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 273. , Verlag Chemie, Deerfield Beach, Florida; Atanasiu, R.L., Stea, D., Mateescu, M.A., Direct evidence of ceruloplasmin antioxidant properties (1998) Mol. Cell. Biochem., 189, pp. 127-129; Bruce, A., Freeman, D., James, C., Biology of disease-free radicals and tissue injury (1982) Lab. Invest., 47, pp. 412-426; Chatterjee, I.B., Nandi, A., Ascorbic acid: A scavenger of oxy radicals (1991) Ind. J. Biochem. Biophys., 28, pp. 233-236; Coscinium fenestratum (1950) The Wealth of India, 2, p. 360. , Publication, Information and Directorate, CSIR, India; Datta, S.C., Mathur, R.K., Baruah, J.N., Minor alkaloids of Coscinium fenestratum root (1988) Indian Drugs, 25 (8), p. 350; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods in Enzymol., 105, pp. 138-142; Ewis, S.A., Abdel Rahman, M.S., Effect of metformin on glutathione and magnesium in normal and TSZ induced diabetic rats (1995) J. Appl. Toxicol., 15, pp. 387-390; Halliwell, B., Gutteridge, J.M.C., Oxygen toxicity: Oxygen radical, transition metal and disease (1984) Biochem. J., 219, pp. 1-14; Hallliwell, B., Gutteridge, J.M.C., (1989) Free Radicals in Biology and Medicine, 2nd Edition, pp. 1-27. , Clarendon Press Publications, Oxford; Harborne, J.B., (1988) Phytochemical Methods, pp. 60-66. , Chapmann & Hall, London; Inefers, H., Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E (1988) Eur. J. Biochem., 174, pp. 353-357; Johann, P., Reinhild, B., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 286. , Verlag Chemie, Deerfield Beach, Florida; Kakkar, P., Dos, B., Viswanathan, R.N., Modified spectrophotometric assay of superoxide dismutase (1984) Ind. J. Biochem. Biophys., 21, pp. 130-132; Kalavincela, T., (1998) Studies on Rural Medicine of Kanyakumari Diatrict with Special Reference to Medicinal Plants, p. 185. , M. Phil Thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India; Kokate, C.K., Purohit, A.P., Gokhale, S.B., (1994) Practical Pharmacognosy, pp. 107-113. , Vallabh Prakashan, New Delhi; Loven, D., Sched, H., Wilson, H., Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with STZ induced (1986) Diabetes, 35, pp. 503-507; Lowry, O.H., Roesborough, M.J., Farr, A.L., Protein measurement with Folin- Phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; Mahapatra, B., Hypoglycemic activity of Coscinium fenestratum (1997) J. Res. Ayu. Siddha, 18, pp. 89-91; Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G., Development of a new model of type 2 diabetes in adult rats administered with Streptozotocin and nicotinamide (1998) Diabetes., 47, pp. 224-228; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD (Eds.), Academic press, New York; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J. Labor. Clin. Med., 589, pp. 161-168; Rotruck, J.T., Pope, A.L., Ganther, H.E., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Saxena, A.K., Srivastava, P., Kale, R.K., Impaired antioxidant status in diabetic rat liver: Effect of vandale (1993) Biochem. Pharmacol., 45, pp. 539-542; Searle, A.J., Wilson, R., Glutathione peroxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity (1980) Int. J. Radiat. Biol., 37, pp. 213-217; Selvam, R., Anuradha, C.V., Effect of oral methionine on blood lipid peroxidation and antioxidants in alloxan induced diabetic rats (1990) J. Nutr. Biochem., 1, pp. 653-658; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in Streptozotocin nicotinamide induced type 2 diabetic rats (2005) J. Ethnopharmacol., 97, pp. 369-374; Singh, G.B., Singh, S., Bani, S., Malhotra, S., Hypotensive action of a Coscinium fenestratum stem extract (1990) J. Ethnopharmacol, 38, pp. 151-155; Sinha, K.A., Colorimetric assay of catalase (1972) Anal. Biochem., 47, pp. 389-1384; Stanely Mainzen Prince, P., Kamalakannan, N., Hypoglycaemic effect of water extracts of Aegle marmelose fruits in Streptozotocin diabetic rats (2003) J. Ethnopharmacol., 87, pp. 207-210; Takenaka, Y., Miki, M., Yasudo, H., The effect of alpha tocopherol as an antioxidant on the oxidation membrane protein thiols induced by free radicals generated in different sites (1991) Arch. Biochem. Biophys., 283, pp. 344-350; Varier, P.S., Coscinium fenestratum (1994) Indian Medicinal Plants, 2, pp. 191-193. , Compendium of 500 species, Orient Longman Ltd., Hyderabad, India; Venukumar, M.R., Latha, M.S., Effect of Coscinium fenestratum on hepatotoxicity in rats (2004) Ind. J. Exp. Biol., 42, pp. 792-797; Wohaieb, S.A., Godin, D.V., Alterations in free radical tissue defense mechanism in STZ induced diabetes in rats. Effects of insulin treatment (1987) Diabetes, 36, pp. 1014-1018",
year = "2005",
language = "English",
volume = "11",
pages = "155--159",
journal = "Natural Product Sciences",
issn = "1226-3907",
publisher = "Korean Society of Pharmacognosy",
number = "3",

}

Punitha, ISR, Bhat, N, Rajendran, K & Shirwaikar, A 2005, 'Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats', Natural Product Sciences, vol. 11, no. 3, pp. 155-159.

Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats. / Punitha, I.S.R.; Bhat, N.; Rajendran, K.; Shirwaikar, A.

In: Natural Product Sciences, Vol. 11, No. 3, 2005, p. 155-159.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant activity of aqueous extract of Coscinium fenestratum in STZ-nicotinamide induced diabetic rats

AU - Punitha, I.S.R.

AU - Bhat, N.

AU - Rajendran, K.

AU - Shirwaikar, A.

N1 - Export Date: 10 November 2017 CODEN: NPSCF Correspondence Address: Shirwaikar, A.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Karnataka, India; email: annieshirwaikar@yahoo.com Chemicals/CAS: ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; ceruloplasmin, 9031-37-2; glutathione peroxidase, 9013-66-5; glutathione synthase, 9023-62-5; nicotinamide, 11032-50-1, 98-92-0; peroxidase, 9003-99-0; streptozocin, 18883-66-4; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; tocopherol, 1406-66-2 References: Aebi, E.H., Catalase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 273. , Verlag Chemie, Deerfield Beach, Florida; Atanasiu, R.L., Stea, D., Mateescu, M.A., Direct evidence of ceruloplasmin antioxidant properties (1998) Mol. Cell. Biochem., 189, pp. 127-129; Bruce, A., Freeman, D., James, C., Biology of disease-free radicals and tissue injury (1982) Lab. Invest., 47, pp. 412-426; Chatterjee, I.B., Nandi, A., Ascorbic acid: A scavenger of oxy radicals (1991) Ind. J. Biochem. Biophys., 28, pp. 233-236; Coscinium fenestratum (1950) The Wealth of India, 2, p. 360. , Publication, Information and Directorate, CSIR, India; Datta, S.C., Mathur, R.K., Baruah, J.N., Minor alkaloids of Coscinium fenestratum root (1988) Indian Drugs, 25 (8), p. 350; Desai, L.D., Vitamin E analysis methods for animal tissues (1984) Methods in Enzymol., 105, pp. 138-142; Ewis, S.A., Abdel Rahman, M.S., Effect of metformin on glutathione and magnesium in normal and TSZ induced diabetic rats (1995) J. Appl. Toxicol., 15, pp. 387-390; Halliwell, B., Gutteridge, J.M.C., Oxygen toxicity: Oxygen radical, transition metal and disease (1984) Biochem. J., 219, pp. 1-14; Hallliwell, B., Gutteridge, J.M.C., (1989) Free Radicals in Biology and Medicine, 2nd Edition, pp. 1-27. , Clarendon Press Publications, Oxford; Harborne, J.B., (1988) Phytochemical Methods, pp. 60-66. , Chapmann & Hall, London; Inefers, H., Sies, H., The protection by ascorbate and glutathione against microsomal lipid peroxidation is dependent on vitamin E (1988) Eur. J. Biochem., 174, pp. 353-357; Johann, P., Reinhild, B., Peroxidase (1983) Bergmeyer Methods of Enzymatic Analysis, Vol. III, 3rd Edition, 3, p. 286. , Verlag Chemie, Deerfield Beach, Florida; Kakkar, P., Dos, B., Viswanathan, R.N., Modified spectrophotometric assay of superoxide dismutase (1984) Ind. J. Biochem. Biophys., 21, pp. 130-132; Kalavincela, T., (1998) Studies on Rural Medicine of Kanyakumari Diatrict with Special Reference to Medicinal Plants, p. 185. , M. Phil Thesis, Manonmaniam Sundaranar University, Tirunelveli, Tamilnadu, India; Kokate, C.K., Purohit, A.P., Gokhale, S.B., (1994) Practical Pharmacognosy, pp. 107-113. , Vallabh Prakashan, New Delhi; Loven, D., Sched, H., Wilson, H., Effect of insulin and oral glutathione on glutathione levels and superoxide dismutase activities in organs of rats with STZ induced (1986) Diabetes, 35, pp. 503-507; Lowry, O.H., Roesborough, M.J., Farr, A.L., Protein measurement with Folin- Phenol reagent (1951) J. Biol. Chem., 193, pp. 265-275; Mahapatra, B., Hypoglycemic activity of Coscinium fenestratum (1997) J. Res. Ayu. Siddha, 18, pp. 89-91; Masiello, P., Broca, C., Gross, R., Roye, M., Manteghetti, M., Hillaire-Buys, D., Novelli, M., Ribes, G., Development of a new model of type 2 diabetes in adult rats administered with Streptozotocin and nicotinamide (1998) Diabetes., 47, pp. 224-228; Omaye, S.T., Turnball, J.D., Sauberlich, H.E., Selected methods for the determination of ascorbic acid in animal cells, tissues and fluids (1979) Methods in Enzymology, 62, pp. 1-11. , McCormick DB, Wright CD (Eds.), Academic press, New York; Ravin, H.A., An improved colorimetric assay of ceruloplasmin (1961) J. Labor. Clin. Med., 589, pp. 161-168; Rotruck, J.T., Pope, A.L., Ganther, H.E., Selenium biochemical role as a component of glutathione peroxide purification and assay (1973) Science, 179, pp. 588-590; Saxena, A.K., Srivastava, P., Kale, R.K., Impaired antioxidant status in diabetic rat liver: Effect of vandale (1993) Biochem. Pharmacol., 45, pp. 539-542; Searle, A.J., Wilson, R., Glutathione peroxide effect of superoxide, hydroxyl and bromine free radicals on enzyme activity (1980) Int. J. Radiat. Biol., 37, pp. 213-217; Selvam, R., Anuradha, C.V., Effect of oral methionine on blood lipid peroxidation and antioxidants in alloxan induced diabetic rats (1990) J. Nutr. Biochem., 1, pp. 653-658; Shirwaikar, A., Rajendran, K., Punitha, I.S.R., Antidiabetic activity of alcoholic stem extract of C. fenestratum in Streptozotocin nicotinamide induced type 2 diabetic rats (2005) J. Ethnopharmacol., 97, pp. 369-374; Singh, G.B., Singh, S., Bani, S., Malhotra, S., Hypotensive action of a Coscinium fenestratum stem extract (1990) J. Ethnopharmacol, 38, pp. 151-155; Sinha, K.A., Colorimetric assay of catalase (1972) Anal. Biochem., 47, pp. 389-1384; Stanely Mainzen Prince, P., Kamalakannan, N., Hypoglycaemic effect of water extracts of Aegle marmelose fruits in Streptozotocin diabetic rats (2003) J. Ethnopharmacol., 87, pp. 207-210; Takenaka, Y., Miki, M., Yasudo, H., The effect of alpha tocopherol as an antioxidant on the oxidation membrane protein thiols induced by free radicals generated in different sites (1991) Arch. Biochem. Biophys., 283, pp. 344-350; Varier, P.S., Coscinium fenestratum (1994) Indian Medicinal Plants, 2, pp. 191-193. , Compendium of 500 species, Orient Longman Ltd., Hyderabad, India; Venukumar, M.R., Latha, M.S., Effect of Coscinium fenestratum on hepatotoxicity in rats (2004) Ind. J. Exp. Biol., 42, pp. 792-797; Wohaieb, S.A., Godin, D.V., Alterations in free radical tissue defense mechanism in STZ induced diabetes in rats. Effects of insulin treatment (1987) Diabetes, 36, pp. 1014-1018

PY - 2005

Y1 - 2005

N2 - The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.

AB - The aqueous extract of Coscinium fenestratum was studied for its antioxidant status in STZ-nicotinamide induced type 2 diabetic rats at two dose levels of 250 mg/kg and 500 mg/kg. At the end of the experimental period, diabetic rats treated with aqueous extract at both dose levels showed a significant increase in the levels of enzymatic antioxidants such as glutathione peroxidase, glutathione synthetase, peroxidase, superoxide dismutase and catalase as compared to the untreated control. Similarly, a significant increase was also observed in the levels of the non enzymatic antioxidants ceruloplasmin, ascorbic acid and tocopherol. The results suggest that the aqueous stem extract of C. fenestratum prevents type 2 diabetes mellitus induced oxidative stress.

M3 - Article

VL - 11

SP - 155

EP - 159

JO - Natural Product Sciences

JF - Natural Product Sciences

SN - 1226-3907

IS - 3

ER -