Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats

R. Govindarajan, M. Vijayakumar, A. Shirwaikar, A.K.S. Rawat, S. Mehrotra, P. Pushpangadan

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.
Original languageEnglish
Pages (from-to)489-496
Number of pages8
JournalActa Pharmaceutica
Volume56
Issue number4
Publication statusPublished - 2006
Externally publishedYes

Fingerprint

Arthritis
Antioxidants
Chlorogenic Acid
Experimental Arthritis
Lipid Peroxides
Solid Phase Extraction
Catalase
Superoxide Dismutase
Glutathione
Anti-Inflammatory Agents
High Pressure Liquid Chromatography

Cite this

Govindarajan, R., Vijayakumar, M., Shirwaikar, A., Rawat, A. K. S., Mehrotra, S., & Pushpangadan, P. (2006). Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats. Acta Pharmaceutica, 56(4), 489-496.
Govindarajan, R. ; Vijayakumar, M. ; Shirwaikar, A. ; Rawat, A.K.S. ; Mehrotra, S. ; Pushpangadan, P. / Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats. In: Acta Pharmaceutica. 2006 ; Vol. 56, No. 4. pp. 489-496.
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title = "Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats",
abstract = "Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.",
author = "R. Govindarajan and M. Vijayakumar and A. Shirwaikar and A.K.S. Rawat and S. Mehrotra and P. Pushpangadan",
note = "Cited By :16 Export Date: 10 November 2017 CODEN: ACPHE Correspondence Address: Shirwaikar, A.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: govind108@yahoo.com Chemicals/CAS: caffeic acid, 27323-69-9, 331-39-5; catalase, 9001-05-2; chlorogenic acid, 327-97-9; glutathione, 70-18-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Catalase, 1.11.1.6; Glutathione, 70-18-8; Phenols; Plant Extracts; Superoxide Dismutase, 1.15.1.1; Thiobarbituric Acid Reactive Substances Manufacturers: Sigma, United States References: Krane, S.M., Conca, W., Stephenson, M.L., Amento, E.P., Goldring, M.B., Mechanisms of matrix degradation in rheumatoid arthritis (1990) Ann. New York Acad. Sci., 580, pp. 340-354; Gutteridge, J.M.C., Antioxidant properties of the proteins ceruloplasmin, albumin and transferrin A. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis (1986) Biochem. Biophys. Acta, 869, pp. 119-127; Aruoma, O.I., Characterization of drugs as antioxidant prophylactics (1996) Free Rad. Biol. Med., 20, pp. 675-705; Holley, A.E., Cheeseman, K.H., Measuring free radical reactions in vivo (1993) Br. Med. Bull., 49, pp. 494-505; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, , CSIR, New Delhi; Ghosh, D., Anandkumar, A., Anti-inflammatory and analgesic activities of gangetin - A pterocarpenoid from Desmodium gangeticum (1981) Indian J. Pharmacol., 15, pp. 391-402; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., The structure and stereochemistry of gangetin. A new pterocarpan from Desmodium gangeticum (1971) J. Chem. Soc., pp. 2420-2422; Ghosal, S., Bhattacharya, S.K., Desmodium alkaloids II, Chemical and pharmacological evaluation of D. gangeticum (1972) Planta Med., 22, pp. 434-440; Govindarajan, R., Rastogi, S., Vijayakumar, M., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Studies on antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull., 26, pp. 1424-1427; Al-Qirim, T.M., Shahwan, M., Zaidi, K.R., Uddin, Q., Banu, N., Effect of khat, its constituents and restraint stress in free radical metabolism of rats (2002) J. Ethnopharmacol., 83, pp. 245-250; Williams, A.S., Camilleir, J.P., Williams, B.D., Suppression of adjuvant induced by liposomally conjugated methotrexate in the rat (1994) Br. J. Rheumat., 33, pp. 530-533; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-Ciocalteau reagent (1951) J. Biol. Chem., 193, pp. 438-448; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Moran, D., De Buitrago, J.M.G., Fernandez, E., Galan, A.I., Munoz, M.E., Jimenez, R., Inhibition of biliary glutathione secretion by cyclosporine A in the rat: Possible mechanisms and role in the cholestasis induced by the drug (1998) J. Hepatol., 29, pp. 68-77; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Indian J. Biochem. Biophys., 21, pp. 130-132; Cohen, G., Dembiec, D., Marcus, J., Measurement of catalase activity in tissue extracts (1970) Anal. Biochem., 34, pp. 30-38; Orsolic, N., Knezevic, A.H., Sver, L., Terzic, S., Basic, I., Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds (2004) J. Ethnopharmacol., 94, pp. 307-315; Park, J.H., Lee, J.K., Kim, H.S., Chung, S.T., Eom, J.H., Kim, K.A., Chung, S.J., Oh, H.Y., Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice (2004) Int. Immunopharmacol., 4, pp. 429-436; Acqua, S.D., Innocenti, G., Antioxidant compounds from Chaerophyllum hirsutum extracts (2004) Fitoterapia, 75, pp. 592-595; McDonald, S., Prenzler, P.D., Antolovich, M., Robards, K., Phenolic content and antioxidant activity of olive extracts (2001) Food Chem., 73, pp. 73-84; Shinmoto, H., Dosako, S., Nakajima, I., Antioxidant activity of bovine lactoferrin on iron/ascorbate induced lipid peroxidation (1992) Biosci. Biotech. Bioch., 56, pp. 2079-2080; Halliwell, B., Free radicals, antioxidants and human disease. Where are we now? (1992) J. Lab. Clin. Med., 119, p. 598",
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Govindarajan, R, Vijayakumar, M, Shirwaikar, A, Rawat, AKS, Mehrotra, S & Pushpangadan, P 2006, 'Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats', Acta Pharmaceutica, vol. 56, no. 4, pp. 489-496.

Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats. / Govindarajan, R.; Vijayakumar, M.; Shirwaikar, A.; Rawat, A.K.S.; Mehrotra, S.; Pushpangadan, P.

In: Acta Pharmaceutica, Vol. 56, No. 4, 2006, p. 489-496.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats

AU - Govindarajan, R.

AU - Vijayakumar, M.

AU - Shirwaikar, A.

AU - Rawat, A.K.S.

AU - Mehrotra, S.

AU - Pushpangadan, P.

N1 - Cited By :16 Export Date: 10 November 2017 CODEN: ACPHE Correspondence Address: Shirwaikar, A.; Pharmacognosy and Ethnopharmacology Division, National Botanical Research Institute, Lucknow-226 001, India; email: govind108@yahoo.com Chemicals/CAS: caffeic acid, 27323-69-9, 331-39-5; catalase, 9001-05-2; chlorogenic acid, 327-97-9; glutathione, 70-18-8; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; Antioxidants; Catalase, 1.11.1.6; Glutathione, 70-18-8; Phenols; Plant Extracts; Superoxide Dismutase, 1.15.1.1; Thiobarbituric Acid Reactive Substances Manufacturers: Sigma, United States References: Krane, S.M., Conca, W., Stephenson, M.L., Amento, E.P., Goldring, M.B., Mechanisms of matrix degradation in rheumatoid arthritis (1990) Ann. New York Acad. Sci., 580, pp. 340-354; Gutteridge, J.M.C., Antioxidant properties of the proteins ceruloplasmin, albumin and transferrin A. A study of their activity in serum and synovial fluid from patients with rheumatoid arthritis (1986) Biochem. Biophys. Acta, 869, pp. 119-127; Aruoma, O.I., Characterization of drugs as antioxidant prophylactics (1996) Free Rad. Biol. Med., 20, pp. 675-705; Holley, A.E., Cheeseman, K.H., Measuring free radical reactions in vivo (1993) Br. Med. Bull., 49, pp. 494-505; Chopra, R.N., Nayar, S.L., Chopra, I.C., (1956) Glossary of Indian Medicinal Plants, , CSIR, New Delhi; Ghosh, D., Anandkumar, A., Anti-inflammatory and analgesic activities of gangetin - A pterocarpenoid from Desmodium gangeticum (1981) Indian J. Pharmacol., 15, pp. 391-402; Purushothman, K.K., Kishore, V.M., Narayanaswamy, V., The structure and stereochemistry of gangetin. A new pterocarpan from Desmodium gangeticum (1971) J. Chem. Soc., pp. 2420-2422; Ghosal, S., Bhattacharya, S.K., Desmodium alkaloids II, Chemical and pharmacological evaluation of D. gangeticum (1972) Planta Med., 22, pp. 434-440; Govindarajan, R., Rastogi, S., Vijayakumar, M., Rawat, A.K.S., Shirwaikar, A., Mehrotra, S., Pushpangadan, P., Studies on antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull., 26, pp. 1424-1427; Al-Qirim, T.M., Shahwan, M., Zaidi, K.R., Uddin, Q., Banu, N., Effect of khat, its constituents and restraint stress in free radical metabolism of rats (2002) J. Ethnopharmacol., 83, pp. 245-250; Williams, A.S., Camilleir, J.P., Williams, B.D., Suppression of adjuvant induced by liposomally conjugated methotrexate in the rat (1994) Br. J. Rheumat., 33, pp. 530-533; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.I., Protein determination using Folin-Ciocalteau reagent (1951) J. Biol. Chem., 193, pp. 438-448; Okhawa, H., Ohishi, N., Yagi, K., Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction (1979) Anal. Biochem., 95, pp. 351-358; Moran, D., De Buitrago, J.M.G., Fernandez, E., Galan, A.I., Munoz, M.E., Jimenez, R., Inhibition of biliary glutathione secretion by cyclosporine A in the rat: Possible mechanisms and role in the cholestasis induced by the drug (1998) J. Hepatol., 29, pp. 68-77; Kakkar, P., Das, B., Viswanathan, P.N., A modified spectrophotometric assay of superoxide dismutase (1984) Indian J. Biochem. Biophys., 21, pp. 130-132; Cohen, G., Dembiec, D., Marcus, J., Measurement of catalase activity in tissue extracts (1970) Anal. Biochem., 34, pp. 30-38; Orsolic, N., Knezevic, A.H., Sver, L., Terzic, S., Basic, I., Immunomodulatory and antimetastatic action of propolis and related polyphenolic compounds (2004) J. Ethnopharmacol., 94, pp. 307-315; Park, J.H., Lee, J.K., Kim, H.S., Chung, S.T., Eom, J.H., Kim, K.A., Chung, S.J., Oh, H.Y., Immunomodulatory effect of caffeic acid phenethyl ester in Balb/c mice (2004) Int. Immunopharmacol., 4, pp. 429-436; Acqua, S.D., Innocenti, G., Antioxidant compounds from Chaerophyllum hirsutum extracts (2004) Fitoterapia, 75, pp. 592-595; McDonald, S., Prenzler, P.D., Antolovich, M., Robards, K., Phenolic content and antioxidant activity of olive extracts (2001) Food Chem., 73, pp. 73-84; Shinmoto, H., Dosako, S., Nakajima, I., Antioxidant activity of bovine lactoferrin on iron/ascorbate induced lipid peroxidation (1992) Biosci. Biotech. Bioch., 56, pp. 2079-2080; Halliwell, B., Free radicals, antioxidants and human disease. Where are we now? (1992) J. Lab. Clin. Med., 119, p. 598

PY - 2006

Y1 - 2006

N2 - Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.

AB - Total alcoholic extract of Desmodium gangeticum, which exhibited significant anti-inflammatory activity, was evaluated for the possible mode of action by studying its antioxidant potential in adjuvant-induced arthritic rats. Activity guided fractionation and isolation were carried out. The phenolics fraction showed maximum potency. Solid phase extraction followed by preparative HPLC of the active phenolic fraction yielded for the first time two potent antioxidant compounds, caffeic acid and chlorogenic acid, from this plant. The biological antioxidant defense system, involving superoxide dismutase, glutathione and catalase, showed a significant increase with their levels close to the normal control with a decrease in the lipid peroxide content upon administration of D. gangeticum extract (100 mg kg-1) and its phenolics (50 mg kg-1) in arthritic rats, thereby indicating the extracts antioxidant property under arthritic conditions.

M3 - Article

VL - 56

SP - 489

EP - 496

JO - Acta Pharmaceutica

JF - Acta Pharmaceutica

SN - 1330-0075

IS - 4

ER -

Govindarajan R, Vijayakumar M, Shirwaikar A, Rawat AKS, Mehrotra S, Pushpangadan P. Antioxidant activity of Desmodium gangeticum and its phenolics in arthritic rats. Acta Pharmaceutica. 2006;56(4):489-496.