Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract

K.R. Prabhakar, V.P. Veerapur, P. Bansal, V.K. Parihar, M. Reddy Kandadi, P. Bhagath Kumar, K.I. Priyadarsini, M.K. Unnikrishnan

Research output: Contribution to journalArticle

38 Citations (Scopus)

Abstract

The ethanolic extract of Pilea microphylla (L.) was defatted, successively fractionated with acetone and the residue so obtained was found to be most potent when subjected to detailed free radical scavenging and in vivo radioprotection studies. The most active fraction reacts with free radicals, such as DPPH (50 μM), ABTS{radical dot}- (100 μM) and {radical dot}OH (generated by Fenton reaction) with IC50 value of 23.15 μg/ml, 3.0 μg/ml and 310 μg/ml, respectively. The most active fraction inhibited iron-induced lipid peroxidation in phosphatidyl choline liposomes with an IC50 of 13.74 μg/ml. The kinetics of scavenging of DPPH and ABTS{radical dot}- radicals were followed at different concentrations of the fraction by employing stopped-flow studies. The observed first order decay rate constants at 200 μg/ml and 50 μg/ml of fraction with DPPH (50 μM) and ABTS{radical dot}- (50 μM) were found to be 0.4 s-1 and 2.1 s-1, respectively. The fraction when screened for in vivo radioprotection in Swiss albino mice showed 80% protection at a dose of 900 mg/kg and with a DRF of about 1.12. The fraction was also found to protect livers of irradiated mice from depletion of endogenous antioxidant enzymes like glutathione, GST, SOD, catalase and thiols. The fraction also protected the villi height, increased the number of crypt cells while offering general protection to the intestine from acute radiation effects. The fraction also protected the hematopoietic system as assessed by endogenous spleen colony assay, contributing to the overall radioprotective ability. © 2006 Elsevier Ireland Ltd. All rights reserved.
Original languageEnglish
Pages (from-to)22-32
Number of pages11
JournalChemico-Biological Interactions
Volume165
Issue number1
DOIs
Publication statusPublished - 2007
Externally publishedYes

Fingerprint

Antioxidants
Scavenging
Inhibitory Concentration 50
Free Radicals
Hematopoietic System
Radiation effects
Radiation Effects
Acetone
Phosphatidylcholines
Ireland
Sulfhydryl Compounds
Liposomes
Liver
Catalase
Lipid Peroxidation
Intestines
Glutathione
Rate constants
Assays
Spleen

Cite this

Prabhakar, K. R., Veerapur, V. P., Bansal, P., Parihar, V. K., Reddy Kandadi, M., Bhagath Kumar, P., ... Unnikrishnan, M. K. (2007). Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract. Chemico-Biological Interactions, 165(1), 22-32. https://doi.org/10.1016/j.cbi.2006.10.007
Prabhakar, K.R. ; Veerapur, V.P. ; Bansal, P. ; Parihar, V.K. ; Reddy Kandadi, M. ; Bhagath Kumar, P. ; Priyadarsini, K.I. ; Unnikrishnan, M.K. / Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract. In: Chemico-Biological Interactions. 2007 ; Vol. 165, No. 1. pp. 22-32.
@article{3b0644e3fa624fbb93d41a980cb95d56,
title = "Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract",
abstract = "The ethanolic extract of Pilea microphylla (L.) was defatted, successively fractionated with acetone and the residue so obtained was found to be most potent when subjected to detailed free radical scavenging and in vivo radioprotection studies. The most active fraction reacts with free radicals, such as DPPH (50 μM), ABTS{radical dot}- (100 μM) and {radical dot}OH (generated by Fenton reaction) with IC50 value of 23.15 μg/ml, 3.0 μg/ml and 310 μg/ml, respectively. The most active fraction inhibited iron-induced lipid peroxidation in phosphatidyl choline liposomes with an IC50 of 13.74 μg/ml. The kinetics of scavenging of DPPH and ABTS{radical dot}- radicals were followed at different concentrations of the fraction by employing stopped-flow studies. The observed first order decay rate constants at 200 μg/ml and 50 μg/ml of fraction with DPPH (50 μM) and ABTS{radical dot}- (50 μM) were found to be 0.4 s-1 and 2.1 s-1, respectively. The fraction when screened for in vivo radioprotection in Swiss albino mice showed 80{\%} protection at a dose of 900 mg/kg and with a DRF of about 1.12. The fraction was also found to protect livers of irradiated mice from depletion of endogenous antioxidant enzymes like glutathione, GST, SOD, catalase and thiols. The fraction also protected the villi height, increased the number of crypt cells while offering general protection to the intestine from acute radiation effects. The fraction also protected the hematopoietic system as assessed by endogenous spleen colony assay, contributing to the overall radioprotective ability. {\circledC} 2006 Elsevier Ireland Ltd. All rights reserved.",
author = "K.R. Prabhakar and V.P. Veerapur and P. Bansal and V.K. Parihar and {Reddy Kandadi}, M. and {Bhagath Kumar}, P. and K.I. Priyadarsini and M.K. Unnikrishnan",
note = "Cited By :37 Export Date: 10 November 2017 CODEN: CBINA Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104 Karnataka, India; email: cheruvaloor@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; cobalt 60, 10198-40-0; glutathione transferase, 50812-37-8; glutathione, 70-18-8; hydroxyl radical, 3352-57-6; mannitol, 69-65-8, 87-78-5; phosphatidylcholine, 55128-59-1, 8002-43-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; trolox C, 56305-04-5; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Antioxidants; Benzothiazoles; Catalase, EC 1.11.1.6; Ethanol, 64-17-5; Free Radical Scavengers; Glutathione, 70-18-8; Phosphatidylcholines; Picrates; Plant Extracts; Radiation-Protective Agents; Sulfonic Acids References: Adelman, R., Saul, R.L., Ames, B., Oxidative damage to DNA: relation to species metabolic rate and life span (1988) Proc. Natl. Acad. Sci. U.S.A., 85, pp. 2706-2708; Roberfroid, M., Calderon, P.B., (1995) Free Radicals and Oxidation Phenomena in Biological Systems, , Dekker, New York pp. 81-263; Pryor, W.A., Porter, N.A., Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids (1990) Free Radic. Biol. Med., 8, pp. 541-543; McCord, J.M., Fridovich, I., Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein) (1969) J. Biol. Chem., 244, pp. 6049-6055; Claiborne, A., Malinowski, D.P., Fridovich, I., Purification and characterization of hydroperoxidase II of Escherichia coli (1979) J. Biol. Chem., 254, pp. 11664-11668; Rikans, L.E., Hornbrook, K.R., Lipid peroxidation, antioxidant protection and aging (1979) Biochim. Biophys. Acta, 31, pp. 116-127; Fang, Y.Z., Yang, S., Wu, G., Free radicals, antioxidants and nutrition (2002) Nutrition, 18, pp. 872-879; Ames, B.A., Gold, L.S., Endogeneous mutagens and the cause of ageing and cancer (review) (1991) Mutat. Res., 250, pp. 3-16; Weiss, J.F., Landauer, M.R., Protection against ionizing radiation by antioxidant nutrients (2003) Toxicology, 189, pp. 1-20; Facey, P.C., Pascoe, K.O., Porter, R.B., Jones, A.D., Investigation of plants used in jamaican folk medicine for anti-bacterial activity (1999) J. Pharm. Pharmacol., 51 (12), pp. 1455-1460; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by the ocimum flavonoids orientin and vicenin: mechanism of action (2000) Radiat. Res., 144, pp. 455-460; Naik, G.H., Priyadarsini, K.I., Bhagirathi, R.G., Mishra, B., Mishra, K.P., Banavalikar, M.M., Mohan, H., In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents (2005) Phytother. Res., 19 (7), pp. 582-586; Prabhakar, K.R., Veerapur, V.P., Vipan Kumar, P., Rao, B.S.S., Priyadarsini, K.I., Unnikrishnan, M.K., Optimization of in vivo radioprotective activity of Coronopus didymus in mice (2006) Int. J. Radiat. Biol., 82 (8), pp. 525-536; Sudheer Kumar, M., Unnikrishnan, M.K., Uma Devi, P., Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow (2003) Mutat. Res., 527, pp. 7-14; Vipan Kumar, P., Prabhakar, K.R., Veerapur, V.P., Sudheer Kumar, M., Rosi Reddy, Y., Mallikarjuna Rao, C., Unnikrishnan, M.K., Sesamol prevents radiation-induced cytotoxicity in Swiss albino mice (2006) Proceedings of the Annual Conference of Society for Free radical Research-India (SFRR-India), P-74, p. 201. , Kolkata; Narla, R.S., Rao, M.N.A., Scavenging of free radicals and inhibition of lipid peroxidation by 3-phenyl syndone (1995) J. Pharm. Pharmacol., 47, pp. 623-625; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int. J. Pharm., 57, pp. 173-176; Sudheer Kumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocos nucifera Linn. (2003) Water Nahrung/Food, 47 (2), pp. 126-131; Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology. 2nd ed., , Scientific Book Agency, Calcutta, India pp. 153-157; Uma Devi, P., Ganasoundari, A., Rao, B.S.S., Srinivasan, K.K., In vivo radioprotection by ocimum flavonoids: survival of mice (1999) Radiat. Res., 151, pp. 74-78; Lowry, O.H., Rosenhrough, N.J., Farr, A.L., Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 270-272; Sedlak, J., Lindsay, R., Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent (1968) Anal. Biochem., 25, pp. 192-205; Habig, W.H., Pabst, M.J., Jarkoby, W.B., Glutathione S-transferases. The first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249 (22), pp. 7130-7139; Claiborne, L., (1985) Handbook of Methods for Oxygen Radical Research, , CRC Press, London p. 22; Misra, H.P., Fridovich, I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase (1972) J. Biol. Chem., 247, pp. 3170-3175; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochim. Biophys. Acta, 1035, pp. 353-360; Braughler, J.M., Chase, R.L., Pregenzer, J.F., Oxidation of ferrous iron during peroxidation of various lipid substrates (1987) Biochem. Biophys. Acta, 921, pp. 457-464; Samarth, R.M., Saini, M.R., Maharwal, J., Dhaka, A., Ashok Kumar, Mentha (Linn.) leaf extract provides protection against radiation-induced alterations in intestinal mucosa of Swiss albino mice (2002) Indian J. Exp. Biol., 40, pp. 1245-1249; Uma Devi, P., Prasanna, P.G.S., Comparative radioprotection of mouse hemopoietic study by some thiols and a polysaccharide (1995) Proc. Natl. Acad. Sci. Lett., 65 B, pp. 89-92; Raskin, Ribnicky, D.M., Komarnytsky, S., Ilic, N., Poulev, A., Borisjuk, N., Brinker, A., Fridlender, B., Plants and human health in the 21st century (2002) Trends Biotechnol., 20 (12), pp. 522-532; Arora, R., Gupta, D., Chawla, R., Sagar, R., Sharma, A., Kumar, R., Prasad, J., Sharma, R.K., Radioprotection by plant products: present status and future prospects (2005) Phytother. Res., 19, pp. 1-22; Karran, P., DNA double stand break and repair in mammalian cell (2000) Curr. Opin. Gen. Dev., 10, pp. 144-150; Bond, V.P., Fliedner, T.M., Archambeau, J.O., (1965) Mammalian Radiation Lethality, , Academic Press, New York pp. 295-312; Denham, J.W., Hauer-Jensen, M., The radiotherapeutic injury a complex {"}wound{"} (2002) Radiother. Oncol., 63, pp. 129-145; Paris, F., Fuks, Z., Kang, A., Capodieci, P., Juan, G., Ehleiter, D., Friedman, A.H., Kolesnick, R., Endothelial apoptosis as the primary lesion initiating intestinal radiation damage in mice (2001) Science, 293, pp. 293-297; Carr, K.E., Effects of radiation damage on intestinal morphology (2001) Int. Rev. Cytol., 208, pp. 1-119; Sun, J., Chen, Y., Li, M., Ge, Z., Role of antioxidant enzymes on ionizing radiation resistance (1998) Free Radic. Biol. Med., 24, pp. 586-593; Navarro, J., Obrador, E., Pellicer, J.A., Aseni, M., Vina, J., Estrela, M., Blood glutathione as an index of radiation-induced oxidative stress in mice and humans (1997) Free Radic. Biol. Med., 22, pp. 1203-1207; Jones, D.P., The role of oxygen concentration in oxidative stress: hypoxic and hyperoxic models (1985) Oxidative Stress, pp. 152-196. , Sies H. (Ed), Academic Press, New York; Meister, A., Anderson, M., Glutathione (1983) Ann. Rev. Biochem., 52, pp. 711-760; Parasassi, T., Giusti, A.M., Gratton, E., Monaco, E., Raimondi, M., Ravagnan, G., Sapora, O., Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation (1994) Int. J. Radiat. Biol., 65, pp. 329-334",
year = "2007",
doi = "10.1016/j.cbi.2006.10.007",
language = "English",
volume = "165",
pages = "22--32",
journal = "Chemico-Biological Interactions",
issn = "0009-2797",
publisher = "Elsevier Ireland Ltd",
number = "1",

}

Prabhakar, KR, Veerapur, VP, Bansal, P, Parihar, VK, Reddy Kandadi, M, Bhagath Kumar, P, Priyadarsini, KI & Unnikrishnan, MK 2007, 'Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract', Chemico-Biological Interactions, vol. 165, no. 1, pp. 22-32. https://doi.org/10.1016/j.cbi.2006.10.007

Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract. / Prabhakar, K.R.; Veerapur, V.P.; Bansal, P.; Parihar, V.K.; Reddy Kandadi, M.; Bhagath Kumar, P.; Priyadarsini, K.I.; Unnikrishnan, M.K.

In: Chemico-Biological Interactions, Vol. 165, No. 1, 2007, p. 22-32.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant and radioprotective effect of the active fraction of Pilea microphylla (L.) ethanolic extract

AU - Prabhakar, K.R.

AU - Veerapur, V.P.

AU - Bansal, P.

AU - Parihar, V.K.

AU - Reddy Kandadi, M.

AU - Bhagath Kumar, P.

AU - Priyadarsini, K.I.

AU - Unnikrishnan, M.K.

N1 - Cited By :37 Export Date: 10 November 2017 CODEN: CBINA Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104 Karnataka, India; email: cheruvaloor@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; catalase, 9001-05-2; cobalt 60, 10198-40-0; glutathione transferase, 50812-37-8; glutathione, 70-18-8; hydroxyl radical, 3352-57-6; mannitol, 69-65-8, 87-78-5; phosphatidylcholine, 55128-59-1, 8002-43-5; superoxide dismutase, 37294-21-6, 9016-01-7, 9054-89-1; trolox C, 56305-04-5; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Antioxidants; Benzothiazoles; Catalase, EC 1.11.1.6; Ethanol, 64-17-5; Free Radical Scavengers; Glutathione, 70-18-8; Phosphatidylcholines; Picrates; Plant Extracts; Radiation-Protective Agents; Sulfonic Acids References: Adelman, R., Saul, R.L., Ames, B., Oxidative damage to DNA: relation to species metabolic rate and life span (1988) Proc. Natl. Acad. Sci. U.S.A., 85, pp. 2706-2708; Roberfroid, M., Calderon, P.B., (1995) Free Radicals and Oxidation Phenomena in Biological Systems, , Dekker, New York pp. 81-263; Pryor, W.A., Porter, N.A., Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids (1990) Free Radic. Biol. Med., 8, pp. 541-543; McCord, J.M., Fridovich, I., Superoxide dismutase, an enzymatic function for erythrocuprein (hemocuprein) (1969) J. Biol. Chem., 244, pp. 6049-6055; Claiborne, A., Malinowski, D.P., Fridovich, I., Purification and characterization of hydroperoxidase II of Escherichia coli (1979) J. Biol. Chem., 254, pp. 11664-11668; Rikans, L.E., Hornbrook, K.R., Lipid peroxidation, antioxidant protection and aging (1979) Biochim. Biophys. Acta, 31, pp. 116-127; Fang, Y.Z., Yang, S., Wu, G., Free radicals, antioxidants and nutrition (2002) Nutrition, 18, pp. 872-879; Ames, B.A., Gold, L.S., Endogeneous mutagens and the cause of ageing and cancer (review) (1991) Mutat. Res., 250, pp. 3-16; Weiss, J.F., Landauer, M.R., Protection against ionizing radiation by antioxidant nutrients (2003) Toxicology, 189, pp. 1-20; Facey, P.C., Pascoe, K.O., Porter, R.B., Jones, A.D., Investigation of plants used in jamaican folk medicine for anti-bacterial activity (1999) J. Pharm. Pharmacol., 51 (12), pp. 1455-1460; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by the ocimum flavonoids orientin and vicenin: mechanism of action (2000) Radiat. Res., 144, pp. 455-460; Naik, G.H., Priyadarsini, K.I., Bhagirathi, R.G., Mishra, B., Mishra, K.P., Banavalikar, M.M., Mohan, H., In vitro antioxidant studies and free radical reactions of triphala, an ayurvedic formulation and its constituents (2005) Phytother. Res., 19 (7), pp. 582-586; Prabhakar, K.R., Veerapur, V.P., Vipan Kumar, P., Rao, B.S.S., Priyadarsini, K.I., Unnikrishnan, M.K., Optimization of in vivo radioprotective activity of Coronopus didymus in mice (2006) Int. J. Radiat. Biol., 82 (8), pp. 525-536; Sudheer Kumar, M., Unnikrishnan, M.K., Uma Devi, P., Effect of 5-aminosalicylic acid on radiation-induced micronuclei in mouse bone marrow (2003) Mutat. Res., 527, pp. 7-14; Vipan Kumar, P., Prabhakar, K.R., Veerapur, V.P., Sudheer Kumar, M., Rosi Reddy, Y., Mallikarjuna Rao, C., Unnikrishnan, M.K., Sesamol prevents radiation-induced cytotoxicity in Swiss albino mice (2006) Proceedings of the Annual Conference of Society for Free radical Research-India (SFRR-India), P-74, p. 201. , Kolkata; Narla, R.S., Rao, M.N.A., Scavenging of free radicals and inhibition of lipid peroxidation by 3-phenyl syndone (1995) J. Pharm. Pharmacol., 47, pp. 623-625; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int. J. Pharm., 57, pp. 173-176; Sudheer Kumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocos nucifera Linn. (2003) Water Nahrung/Food, 47 (2), pp. 126-131; Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Ghosh, M.N., (1984) Fundamentals of Experimental Pharmacology. 2nd ed., , Scientific Book Agency, Calcutta, India pp. 153-157; Uma Devi, P., Ganasoundari, A., Rao, B.S.S., Srinivasan, K.K., In vivo radioprotection by ocimum flavonoids: survival of mice (1999) Radiat. Res., 151, pp. 74-78; Lowry, O.H., Rosenhrough, N.J., Farr, A.L., Randall, Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem., 193, pp. 270-272; Sedlak, J., Lindsay, R., Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman's reagent (1968) Anal. Biochem., 25, pp. 192-205; Habig, W.H., Pabst, M.J., Jarkoby, W.B., Glutathione S-transferases. The first enzymatic step in mercapturic acid formation (1974) J. Biol. Chem., 249 (22), pp. 7130-7139; Claiborne, L., (1985) Handbook of Methods for Oxygen Radical Research, , CRC Press, London p. 22; Misra, H.P., Fridovich, I., The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase (1972) J. Biol. Chem., 247, pp. 3170-3175; Gelvan, D., Saltman, P., Different cellular targets of Cu- and Fe-catalyzed oxidation observed using a Cu-compatible thiobarbiturate acid assay (1990) Biochim. Biophys. Acta, 1035, pp. 353-360; Braughler, J.M., Chase, R.L., Pregenzer, J.F., Oxidation of ferrous iron during peroxidation of various lipid substrates (1987) Biochem. Biophys. Acta, 921, pp. 457-464; Samarth, R.M., Saini, M.R., Maharwal, J., Dhaka, A., Ashok Kumar, Mentha (Linn.) leaf extract provides protection against radiation-induced alterations in intestinal mucosa of Swiss albino mice (2002) Indian J. Exp. Biol., 40, pp. 1245-1249; Uma Devi, P., Prasanna, P.G.S., Comparative radioprotection of mouse hemopoietic study by some thiols and a polysaccharide (1995) Proc. Natl. Acad. Sci. Lett., 65 B, pp. 89-92; Raskin, Ribnicky, D.M., Komarnytsky, S., Ilic, N., Poulev, A., Borisjuk, N., Brinker, A., Fridlender, B., Plants and human health in the 21st century (2002) Trends Biotechnol., 20 (12), pp. 522-532; Arora, R., Gupta, D., Chawla, R., Sagar, R., Sharma, A., Kumar, R., Prasad, J., Sharma, R.K., Radioprotection by plant products: present status and future prospects (2005) Phytother. Res., 19, pp. 1-22; Karran, P., DNA double stand break and repair in mammalian cell (2000) Curr. Opin. Gen. Dev., 10, pp. 144-150; Bond, V.P., Fliedner, T.M., Archambeau, J.O., (1965) Mammalian Radiation Lethality, , Academic Press, New York pp. 295-312; Denham, J.W., Hauer-Jensen, M., The radiotherapeutic injury a complex "wound" (2002) Radiother. Oncol., 63, pp. 129-145; Paris, F., Fuks, Z., Kang, A., Capodieci, P., Juan, G., Ehleiter, D., Friedman, A.H., Kolesnick, R., Endothelial apoptosis as the primary lesion initiating intestinal radiation damage in mice (2001) Science, 293, pp. 293-297; Carr, K.E., Effects of radiation damage on intestinal morphology (2001) Int. Rev. Cytol., 208, pp. 1-119; Sun, J., Chen, Y., Li, M., Ge, Z., Role of antioxidant enzymes on ionizing radiation resistance (1998) Free Radic. Biol. Med., 24, pp. 586-593; Navarro, J., Obrador, E., Pellicer, J.A., Aseni, M., Vina, J., Estrela, M., Blood glutathione as an index of radiation-induced oxidative stress in mice and humans (1997) Free Radic. Biol. Med., 22, pp. 1203-1207; Jones, D.P., The role of oxygen concentration in oxidative stress: hypoxic and hyperoxic models (1985) Oxidative Stress, pp. 152-196. , Sies H. (Ed), Academic Press, New York; Meister, A., Anderson, M., Glutathione (1983) Ann. Rev. Biochem., 52, pp. 711-760; Parasassi, T., Giusti, A.M., Gratton, E., Monaco, E., Raimondi, M., Ravagnan, G., Sapora, O., Evidence for an increase in water concentration in bilayers after oxidative damage of phospholipids induced by ionizing radiation (1994) Int. J. Radiat. Biol., 65, pp. 329-334

PY - 2007

Y1 - 2007

N2 - The ethanolic extract of Pilea microphylla (L.) was defatted, successively fractionated with acetone and the residue so obtained was found to be most potent when subjected to detailed free radical scavenging and in vivo radioprotection studies. The most active fraction reacts with free radicals, such as DPPH (50 μM), ABTS{radical dot}- (100 μM) and {radical dot}OH (generated by Fenton reaction) with IC50 value of 23.15 μg/ml, 3.0 μg/ml and 310 μg/ml, respectively. The most active fraction inhibited iron-induced lipid peroxidation in phosphatidyl choline liposomes with an IC50 of 13.74 μg/ml. The kinetics of scavenging of DPPH and ABTS{radical dot}- radicals were followed at different concentrations of the fraction by employing stopped-flow studies. The observed first order decay rate constants at 200 μg/ml and 50 μg/ml of fraction with DPPH (50 μM) and ABTS{radical dot}- (50 μM) were found to be 0.4 s-1 and 2.1 s-1, respectively. The fraction when screened for in vivo radioprotection in Swiss albino mice showed 80% protection at a dose of 900 mg/kg and with a DRF of about 1.12. The fraction was also found to protect livers of irradiated mice from depletion of endogenous antioxidant enzymes like glutathione, GST, SOD, catalase and thiols. The fraction also protected the villi height, increased the number of crypt cells while offering general protection to the intestine from acute radiation effects. The fraction also protected the hematopoietic system as assessed by endogenous spleen colony assay, contributing to the overall radioprotective ability. © 2006 Elsevier Ireland Ltd. All rights reserved.

AB - The ethanolic extract of Pilea microphylla (L.) was defatted, successively fractionated with acetone and the residue so obtained was found to be most potent when subjected to detailed free radical scavenging and in vivo radioprotection studies. The most active fraction reacts with free radicals, such as DPPH (50 μM), ABTS{radical dot}- (100 μM) and {radical dot}OH (generated by Fenton reaction) with IC50 value of 23.15 μg/ml, 3.0 μg/ml and 310 μg/ml, respectively. The most active fraction inhibited iron-induced lipid peroxidation in phosphatidyl choline liposomes with an IC50 of 13.74 μg/ml. The kinetics of scavenging of DPPH and ABTS{radical dot}- radicals were followed at different concentrations of the fraction by employing stopped-flow studies. The observed first order decay rate constants at 200 μg/ml and 50 μg/ml of fraction with DPPH (50 μM) and ABTS{radical dot}- (50 μM) were found to be 0.4 s-1 and 2.1 s-1, respectively. The fraction when screened for in vivo radioprotection in Swiss albino mice showed 80% protection at a dose of 900 mg/kg and with a DRF of about 1.12. The fraction was also found to protect livers of irradiated mice from depletion of endogenous antioxidant enzymes like glutathione, GST, SOD, catalase and thiols. The fraction also protected the villi height, increased the number of crypt cells while offering general protection to the intestine from acute radiation effects. The fraction also protected the hematopoietic system as assessed by endogenous spleen colony assay, contributing to the overall radioprotective ability. © 2006 Elsevier Ireland Ltd. All rights reserved.

U2 - 10.1016/j.cbi.2006.10.007

DO - 10.1016/j.cbi.2006.10.007

M3 - Article

VL - 165

SP - 22

EP - 32

JO - Chemico-Biological Interactions

JF - Chemico-Biological Interactions

SN - 0009-2797

IS - 1

ER -