Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir

H.N. Aswatha Ram, C.S. Shreedhara, P. Falguni Gajera, B. Sachin Zanwar

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Free radicals are implicated for many diseases including Diabetes mellitus, arthritis, cancer, ageing. etc. In treatment of these diseases, antioxidant therapy has gained utmost importance. Phyllanthus reticulatus Poir (Euphrobiaceae) popularly known as 'potato-bush' is an important medicinal plant and useful in burning sensation, strangury, gastropathy, ophthalmodynia, burns, diarrhoea, skin eruption, diabetes and obesity. Keeping in view of the cited activity, it is contemplated to screen the plant for in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, iron chelating activity and lipid peroxidation assay, nitric oxide scavenging assay, alkaline DMSO assay, total antioxidant capacity and non-enzymatic haemoglobin glycosylation assay . The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 20.36 μgm/ml (DPPH radical scavenging), 42.59 μgm/ml (ABTS radical scavenging), 32 μgm/ml (Iron chelating activity) and 41.91 μgm/ml (lipid peroxidation), 122.8 μgm/ ml (nitric oxide scavenging) and 2.57 μgm/ml (alkaline DMSO). In total antioxidant capacity assay, 1 mg of extract is equivalent to 51 μg of ascorbic acid. It showed 66.64% inhibition of haemoglobin glycosylation. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Phyllanthus reticulatus is effective against free radical mediated diseases.
Original languageEnglish
Pages (from-to)351-364
Number of pages14
JournalPharmacologyonline
Volume1
Publication statusPublished - 2008

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Phyllanthus
Antioxidants
Lipid Peroxidation
Free Radicals
Dimethyl Sulfoxide
Glycosylation
Nitric Oxide
Hemoglobins
Iron
Polyphenols
Medicinal Plants
Solanum tuberosum
Burns
Flavonoids
Ascorbic Acid
Inhibitory Concentration 50
Arthritis
Diarrhea
Diabetes Mellitus
Obesity

Cite this

Aswatha Ram, H. N., Shreedhara, C. S., Falguni Gajera, P., & Sachin Zanwar, B. (2008). Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir. Pharmacologyonline, 1, 351-364.
Aswatha Ram, H.N. ; Shreedhara, C.S. ; Falguni Gajera, P. ; Sachin Zanwar, B. / Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir. In: Pharmacologyonline. 2008 ; Vol. 1. pp. 351-364.
@article{80ff3198642d4345bf2e31cab7909301,
title = "Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir",
abstract = "Free radicals are implicated for many diseases including Diabetes mellitus, arthritis, cancer, ageing. etc. In treatment of these diseases, antioxidant therapy has gained utmost importance. Phyllanthus reticulatus Poir (Euphrobiaceae) popularly known as 'potato-bush' is an important medicinal plant and useful in burning sensation, strangury, gastropathy, ophthalmodynia, burns, diarrhoea, skin eruption, diabetes and obesity. Keeping in view of the cited activity, it is contemplated to screen the plant for in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, iron chelating activity and lipid peroxidation assay, nitric oxide scavenging assay, alkaline DMSO assay, total antioxidant capacity and non-enzymatic haemoglobin glycosylation assay . The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 20.36 μgm/ml (DPPH radical scavenging), 42.59 μgm/ml (ABTS radical scavenging), 32 μgm/ml (Iron chelating activity) and 41.91 μgm/ml (lipid peroxidation), 122.8 μgm/ ml (nitric oxide scavenging) and 2.57 μgm/ml (alkaline DMSO). In total antioxidant capacity assay, 1 mg of extract is equivalent to 51 μg of ascorbic acid. It showed 66.64{\%} inhibition of haemoglobin glycosylation. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Phyllanthus reticulatus is effective against free radical mediated diseases.",
author = "{Aswatha Ram}, H.N. and C.S. Shreedhara and {Falguni Gajera}, P. and {Sachin Zanwar}, B.",
note = "Cited By :15 Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104 Karnataka, India; email: aswatharam@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; dimethyl sulfoxide, 67-68-5; hemoglobin, 9008-02-0; polyphenol, 37331-26-3 References: Gutteridgdem, J.M.C., Free radicals in disease processes: A complication of cause and consequence (1995) Free Radic. Res. Comm, 19, pp. 141-158; Tiwari, A., Imbalance in antioxidant defense and human diseases: Multiple approach of natural antioxidants therapy (2001) Curr. sci, 81, pp. 1179-1187; Joyce, D.A., Oxygen radicals in disease (1987) Adv. Drug Reac. Bull, 127, pp. 476-479; Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D., Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products (1998) Journal of Agricultural Food & Chemistry, 46, pp. 4113-4117; Pietta, P.G., Flavonoids in medicinal plants (1998) Evans and Packer L), pp. 61-110. , CA Rice ed, New York: Dekker; Shahidi, F., Janitha, P.K., Wanasundara, P.D., Phenolic antioxidants (1992) Critical Reviews of Food Science & Nutrition, 32, pp. 67-103; Osawa, T. Novel natural antioxidants for utilization in food and biological systems. In I. Uritani- Postharvest biochemistry of plant food-materials in the tropics (ed. VV Garcia and EM Mendoza), Tokyo, Japan: Japan Scientific Societies Press, 1994: 241-251; (1987) Medicinal Plants of India, 2, p. 407. , ICMR, Indian council of medicinal research, New Delhi; Nadkarni, K.M., Nadkarni, A.K., (1976) Indian Materia Medica, 1, pp. 948-949. , Popular Prakashan Pvt. Ltd, Bombay, India; Longman, O., (2003) Indian medicinal plants, a compendium of 500 species, 4, p. 264; Lam, S.-H., Wang, C.-Y., Chen, C.-K., Lee, S.-S., Phytochemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods (2007) Phytochemical Analysis, 18, pp. 251-255; Ghani, A., (2003) Medicinal Plants of Bangladesh: Chemical Constituents and Uses, p. 345. , Asiatic Society of Bangladesh, 2nd edition; Kumar, S., Kumar, D., Deshmukh, R.R., Lokhande, P.D., More, S.N., Rangari, V.D., Antidiabetic potential of Phyllanthus reticulatus in alloxan-induced diabetic mice (2008) Fitoterapia, 79, pp. 21-23; Singleton, V.L., Rossi, Colorimetry of total phenolics with phosphomolybdic acid - phosphotungtic acid reagents (1965) Am. J. Enol. Viticult, 16, pp. 44-158; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; John, A., steven, D.A., Microsomal lipid peroxidation (1984) Methods in Enzymology, 30, pp. 302-308; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem 1996; 239: 70; Sudheerkumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocus nucifera Linn (2003) Nahrung/Food, 47, pp. 126-131; Govindarajan, R., Vijaya Kumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royle ex Benth (2003) Indian J. Exptl. Biol, 41, p. 875; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., Mehlotra, S., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull, 26, p. 1424; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and Rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68, pp. 256-258; Ganapaty, S., Chandrashekhar, V.M., Chitme, H.R., Lakshmi Narsu, M., Free radical scavenging activity of gossypin and nevadensin : An in vitro relation (2007) Indian journal of Pharmacology, 39, pp. 281-283; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol. Pharm. Bull, 27, pp. 38-46; Valentao, P., Fernandes, E., Carvalho, F., Andrade, P.B., Seabra, R.M., Bastos, M.L., Studies on the antioxidant activity of Lippia citriodoru Infusion: Scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol. Pharm. Bull, 25, pp. 1324-1327; Riemersma, R.A., Carruthers, K.F., Elton, R.A., Fox, K.A., Vitamin-C and the risk of acute myocardial infarction (2000) Am. J. Clin. Nutr, 179, pp. 1181-1186; Hagerman, A.E., Riedl, K.M., Jones, G.A., sovik, K.N., Ritchard, N.T., Hartzfeld, P.W., High molecular weight plant polyphenolics (tannins) as biological antioxidants (1998) J. Agric. And Food Chem, 46, pp. 1887-1892; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant actions of Ginko biloba extract Egb 761 (1958) Method in Enzymol, 26, pp. 1199-1202; Shirwaiar, A., Arun Shirwaikar, I.S.R.P., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural Product Sciences, 13 (1), pp. 40-45",
year = "2008",
language = "English",
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pages = "351--364",
journal = "Pharmacologyonline",
issn = "1827-8620",
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}

Aswatha Ram, HN, Shreedhara, CS, Falguni Gajera, P & Sachin Zanwar, B 2008, 'Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir', Pharmacologyonline, vol. 1, pp. 351-364.

Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir. / Aswatha Ram, H.N.; Shreedhara, C.S.; Falguni Gajera, P.; Sachin Zanwar, B.

In: Pharmacologyonline, Vol. 1, 2008, p. 351-364.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir

AU - Aswatha Ram, H.N.

AU - Shreedhara, C.S.

AU - Falguni Gajera, P.

AU - Sachin Zanwar, B.

N1 - Cited By :15 Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal - 576 104 Karnataka, India; email: aswatharam@yahoo.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; dimethyl sulfoxide, 67-68-5; hemoglobin, 9008-02-0; polyphenol, 37331-26-3 References: Gutteridgdem, J.M.C., Free radicals in disease processes: A complication of cause and consequence (1995) Free Radic. Res. Comm, 19, pp. 141-158; Tiwari, A., Imbalance in antioxidant defense and human diseases: Multiple approach of natural antioxidants therapy (2001) Curr. sci, 81, pp. 1179-1187; Joyce, D.A., Oxygen radicals in disease (1987) Adv. Drug Reac. Bull, 127, pp. 476-479; Velioglu, Y.S., Mazza, G., Gao, L., Oomah, B.D., Antioxidant activity and total phenolics in selected fruits, vegetables, and grain products (1998) Journal of Agricultural Food & Chemistry, 46, pp. 4113-4117; Pietta, P.G., Flavonoids in medicinal plants (1998) Evans and Packer L), pp. 61-110. , CA Rice ed, New York: Dekker; Shahidi, F., Janitha, P.K., Wanasundara, P.D., Phenolic antioxidants (1992) Critical Reviews of Food Science & Nutrition, 32, pp. 67-103; Osawa, T. Novel natural antioxidants for utilization in food and biological systems. In I. Uritani- Postharvest biochemistry of plant food-materials in the tropics (ed. VV Garcia and EM Mendoza), Tokyo, Japan: Japan Scientific Societies Press, 1994: 241-251; (1987) Medicinal Plants of India, 2, p. 407. , ICMR, Indian council of medicinal research, New Delhi; Nadkarni, K.M., Nadkarni, A.K., (1976) Indian Materia Medica, 1, pp. 948-949. , Popular Prakashan Pvt. Ltd, Bombay, India; Longman, O., (2003) Indian medicinal plants, a compendium of 500 species, 4, p. 264; Lam, S.-H., Wang, C.-Y., Chen, C.-K., Lee, S.-S., Phytochemical investigation of Phyllanthus reticulatus by HPLC-SPE-NMR and conventional methods (2007) Phytochemical Analysis, 18, pp. 251-255; Ghani, A., (2003) Medicinal Plants of Bangladesh: Chemical Constituents and Uses, p. 345. , Asiatic Society of Bangladesh, 2nd edition; Kumar, S., Kumar, D., Deshmukh, R.R., Lokhande, P.D., More, S.N., Rangari, V.D., Antidiabetic potential of Phyllanthus reticulatus in alloxan-induced diabetic mice (2008) Fitoterapia, 79, pp. 21-23; Singleton, V.L., Rossi, Colorimetry of total phenolics with phosphomolybdic acid - phosphotungtic acid reagents (1965) Am. J. Enol. Viticult, 16, pp. 44-158; Sreejayan, N., Rao, M.N.A., Free radical scavenging activity of curcuminoids (1996) Drug Res, 46, p. 169; John, A., steven, D.A., Microsomal lipid peroxidation (1984) Methods in Enzymology, 30, pp. 302-308; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem 1996; 239: 70; Sudheerkumar, M., Jagadish, P.C., Sridhar, R.B., Kiran, B.S., Unnikrishnan, M.K., In vitro evaluation of antioxidant properties of Cocus nucifera Linn (2003) Nahrung/Food, 47, pp. 126-131; Govindarajan, R., Vijaya Kumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royle ex Benth (2003) Indian J. Exptl. Biol, 41, p. 875; Annie, S., Govindrajan, R., Rastogi, S., Vijaykumar, M., Rawat, A.K.S., Mehlotra, S., Pushpangadan, P., Studies on the Antioxidant activities of Desmodium gangeticum (2003) Biol. Pharm. Bull, 26, p. 1424; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and Rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68, pp. 256-258; Ganapaty, S., Chandrashekhar, V.M., Chitme, H.R., Lakshmi Narsu, M., Free radical scavenging activity of gossypin and nevadensin : An in vitro relation (2007) Indian journal of Pharmacology, 39, pp. 281-283; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol. Pharm. Bull, 27, pp. 38-46; Valentao, P., Fernandes, E., Carvalho, F., Andrade, P.B., Seabra, R.M., Bastos, M.L., Studies on the antioxidant activity of Lippia citriodoru Infusion: Scavenging effect on superoxide radical, hydroxyl radical and hypochlorous acid (2002) Biol. Pharm. Bull, 25, pp. 1324-1327; Riemersma, R.A., Carruthers, K.F., Elton, R.A., Fox, K.A., Vitamin-C and the risk of acute myocardial infarction (2000) Am. J. Clin. Nutr, 179, pp. 1181-1186; Hagerman, A.E., Riedl, K.M., Jones, G.A., sovik, K.N., Ritchard, N.T., Hartzfeld, P.W., High molecular weight plant polyphenolics (tannins) as biological antioxidants (1998) J. Agric. And Food Chem, 46, pp. 1887-1892; Marcocci, L., Packer, L., Droy-Lefaiz, M.T., Sekaki, A., Gardes-Albert, M., Antioxidant actions of Ginko biloba extract Egb 761 (1958) Method in Enzymol, 26, pp. 1199-1202; Shirwaiar, A., Arun Shirwaikar, I.S.R.P., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural Product Sciences, 13 (1), pp. 40-45

PY - 2008

Y1 - 2008

N2 - Free radicals are implicated for many diseases including Diabetes mellitus, arthritis, cancer, ageing. etc. In treatment of these diseases, antioxidant therapy has gained utmost importance. Phyllanthus reticulatus Poir (Euphrobiaceae) popularly known as 'potato-bush' is an important medicinal plant and useful in burning sensation, strangury, gastropathy, ophthalmodynia, burns, diarrhoea, skin eruption, diabetes and obesity. Keeping in view of the cited activity, it is contemplated to screen the plant for in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, iron chelating activity and lipid peroxidation assay, nitric oxide scavenging assay, alkaline DMSO assay, total antioxidant capacity and non-enzymatic haemoglobin glycosylation assay . The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 20.36 μgm/ml (DPPH radical scavenging), 42.59 μgm/ml (ABTS radical scavenging), 32 μgm/ml (Iron chelating activity) and 41.91 μgm/ml (lipid peroxidation), 122.8 μgm/ ml (nitric oxide scavenging) and 2.57 μgm/ml (alkaline DMSO). In total antioxidant capacity assay, 1 mg of extract is equivalent to 51 μg of ascorbic acid. It showed 66.64% inhibition of haemoglobin glycosylation. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Phyllanthus reticulatus is effective against free radical mediated diseases.

AB - Free radicals are implicated for many diseases including Diabetes mellitus, arthritis, cancer, ageing. etc. In treatment of these diseases, antioxidant therapy has gained utmost importance. Phyllanthus reticulatus Poir (Euphrobiaceae) popularly known as 'potato-bush' is an important medicinal plant and useful in burning sensation, strangury, gastropathy, ophthalmodynia, burns, diarrhoea, skin eruption, diabetes and obesity. Keeping in view of the cited activity, it is contemplated to screen the plant for in vitro antioxidant activity using different models viz. DPPH radical scavenging, ABTS radical scavenging, iron chelating activity and lipid peroxidation assay, nitric oxide scavenging assay, alkaline DMSO assay, total antioxidant capacity and non-enzymatic haemoglobin glycosylation assay . The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 20.36 μgm/ml (DPPH radical scavenging), 42.59 μgm/ml (ABTS radical scavenging), 32 μgm/ml (Iron chelating activity) and 41.91 μgm/ml (lipid peroxidation), 122.8 μgm/ ml (nitric oxide scavenging) and 2.57 μgm/ml (alkaline DMSO). In total antioxidant capacity assay, 1 mg of extract is equivalent to 51 μg of ascorbic acid. It showed 66.64% inhibition of haemoglobin glycosylation. In all the testing, a significant correlation existed between concentrations of the extract and percentage inhibition of free radicals, metal chelation or inhibition of lipid peroxidation. The antioxidant property may be related to the polyphenols and flavonoids present in the extract. These results clearly indicate that Phyllanthus reticulatus is effective against free radical mediated diseases.

M3 - Article

VL - 1

SP - 351

EP - 364

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -

Aswatha Ram HN, Shreedhara CS, Falguni Gajera P, Sachin Zanwar B. Antioxidant studies of aqueous extract of Phyllanthus reticulatus Poir. Pharmacologyonline. 2008;1:351-364.