Antioxidant studies on the ethanolic extract of Zornia gibbosa

S.N. Laxane, S.K. Swarnkar, M.M. Setty

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Abstract

The ethanolic extract of Zornia zibbosa, is widely used in the indigenous system of medicine was studied for its in vitro scavenging activity in different methods viz. DPPH, ABTS radical scavenging, lipid peroxidation, iron chelation activity, superoxide scavenging, total antioxidant capacity and antioxidant haemoglobin glycosylation. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 14.78μg/ml for lipid peroxidation, 40.29 μg/ml for ABTS scavenging, 83.11μg/ml for iron chelating activity, 105.90μg/ml for DPPH scavenging and 97.96μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 17.96 μg/ml. In Haemaglobin glycosylation model the % scavenging was found to be 45.49% and 74.68% at the concentmtion 0.5 mg/ml and 1.0 mg/ml respectively. In all the methods the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Z. Gibbosa has significant antioxidant activity.
Original languageEnglish
Pages (from-to)319-330
Number of pages12
JournalPharmacologyonline
Volume1
Publication statusPublished - 2008

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Antioxidants
Glycosylation
Superoxides
Lipid Peroxidation
Iron
Traditional Medicine
Systems Analysis
Inhibitory Concentration 50
Free Radicals
Hemoglobins
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid

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Laxane, S.N. ; Swarnkar, S.K. ; Setty, M.M. / Antioxidant studies on the ethanolic extract of Zornia gibbosa. In: Pharmacologyonline. 2008 ; Vol. 1. pp. 319-330.
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abstract = "The ethanolic extract of Zornia zibbosa, is widely used in the indigenous system of medicine was studied for its in vitro scavenging activity in different methods viz. DPPH, ABTS radical scavenging, lipid peroxidation, iron chelation activity, superoxide scavenging, total antioxidant capacity and antioxidant haemoglobin glycosylation. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 14.78μg/ml for lipid peroxidation, 40.29 μg/ml for ABTS scavenging, 83.11μg/ml for iron chelating activity, 105.90μg/ml for DPPH scavenging and 97.96μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 17.96 μg/ml. In Haemaglobin glycosylation model the {\%} scavenging was found to be 45.49{\%} and 74.68{\%} at the concentmtion 0.5 mg/ml and 1.0 mg/ml respectively. In all the methods the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Z. Gibbosa has significant antioxidant activity.",
author = "S.N. Laxane and S.K. Swarnkar and M.M. Setty",
note = "Cited By :1 Export Date: 10 November 2017 Correspondence Address: Setty, M.M.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576 104 Karnataka, India; email: mm.setty@manipal.edu Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; alcohol, 64-17-5; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; iron, 14093-02-8, 53858-86-9, 7439-89-6; superoxide, 11062-77-4 References: Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural product sciences, 13 (1), pp. 40-45; Yoganarasimhan, S.N., (2000) Medicinal Plants of India, 2, p. 606. , Tamilnadu. 1st ed. Bangalore: Cyber Media publications; Molyneux P. The use of stable free radical diphenyl picryl hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. and technol. 2004; 26(2):212-219; Schlesier, K., Harwart, M., Bohm, V., Bitsch, R., Assessment of antioxidant activity by using different in vitro methods (2002) Free radic. Res, 30 (2), pp. 177-185; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Antioxidant activity applying an improved ABTS radical cation decolorization assay (1998) Free Radic. Biol. Med, 72, pp. 1231-1237; Blois, Antioxidant determinations by the use of stable free radical (1958) Nature, 26, pp. 1199-1201; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem. 1996; 239:70-76; Benzie, I.F.F., Szeto, Y.T., Total antioxidant capacity of teas by the ferric reducing antioxidant power assay (1999) J. Agri. Food Chem, 47, pp. 633-636; Govindrarajan, R., Vijaykumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royale ex Benth (2003) Indian J.Exptl Biol, 41, pp. 875-875; Mruthunjaya, K., Hukkeri, V.I., (2008) In vitro Antioxidant and free radical scavenging potential of Parkinsonia aculeata Linn PHCOG MAG, 4 (13), pp. 42-51; Shirwaikar, A., Rajendra, K., Dinesh Kumar, C., Invitro antioxidant studies of Annona squamosa Linn, Leaves (2004) Indian J. Exptl. Biol, 42, pp. 803-807; Preito, P., Pinedo, M., Aguilar, M., Spectrophotometric quantification of antioxidant capacity through the formation of the phosphomolybdenum complex: Specific application to the determination of vitamin E (1999) Anal. Biochem, 269, pp. 337-341; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68 (2), pp. 256-258; Cross, C.E., Oxygen Radical and human disease, Annals of Internal. Med (1987), 107, pp. 526-529; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and Antioxidative system in plants (2002) Cur. Sci, 82, pp. 1227-1229; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate induced lipid peroxidition in cultured human dermal fibroblasts (1990) Arch. Biochem. Biphy, 278, pp. 352-355; Badmis, S., Gupta, M.K., Suresh, B., Antioxidant activity of the ethanolic extract of Striga orobanchiodes (2003) J. Ethanopharmacol, 85, pp. 227-230; Sanchez-Morino, C., Method used to evaluate the free radical scavenging activity in foods and biological system (2002) Food Sci. And Technol. Int, 8, pp. 122-126; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol.Pharm. Bull, 27 (1), pp. 38-46; Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., Antioxidant activity of Sesame coat (2002) Food Chem, 78, pp. 347-354; Halliwell, B., Reactive oxygen species in living systems: Source,biochemistry and role in human disease (1991) Am. J. Med, 91, pp. 14S-22S; Duh, P.D., Tu, Y.Y., Yen, G.C., Antioxidant acitvity of aqueous extract of harn jyur (Chrysanthemum morifolium Ramat) (1999) Lebensmittel-Wissenschaft und Technologie, 32, pp. 269-277; Gordon, M.H., The mechanism of the antioxidint action in vitro (1990) Food antioxidants, pp. 1-18. , Hudson, BJF Ed, Ekseveir, London; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Cross, A.R., Jones, O.T.G., Enzymic mechanism of superoxide (1991) Biochem. Biophys. Acta, 387, pp. 281-285; Wiseman, H., Halliwell, B., Damage to DNA by reactive oxygen and nitrogen species: Role in in inflammatory disease and progression to cancer (1996) Biochem. J, 313, pp. 17-19; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., In vitro antioxidant studies of Sphaeranthus indicus (Linn) (2006) Indian J. Exptl. Biol, 44, pp. 993-996",
year = "2008",
language = "English",
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pages = "319--330",
journal = "Pharmacologyonline",
issn = "1827-8620",
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}

Antioxidant studies on the ethanolic extract of Zornia gibbosa. / Laxane, S.N.; Swarnkar, S.K.; Setty, M.M.

In: Pharmacologyonline, Vol. 1, 2008, p. 319-330.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Antioxidant studies on the ethanolic extract of Zornia gibbosa

AU - Laxane, S.N.

AU - Swarnkar, S.K.

AU - Setty, M.M.

N1 - Cited By :1 Export Date: 10 November 2017 Correspondence Address: Setty, M.M.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal University, Manipal-576 104 Karnataka, India; email: mm.setty@manipal.edu Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; alcohol, 64-17-5; alpha tocopherol, 1406-18-4, 1406-70-8, 52225-20-4, 58-95-7, 59-02-9; ascorbic acid, 134-03-2, 15421-15-5, 50-81-7; hemoglobin, 9008-02-0; iron, 14093-02-8, 53858-86-9, 7439-89-6; superoxide, 11062-77-4 References: Shirwaikar, A., Shirwaikar, A., Punitha, I.S.R., Antioxidant studies on the methanol stem extract of Coscinium fenestratum (2007) Natural product sciences, 13 (1), pp. 40-45; Yoganarasimhan, S.N., (2000) Medicinal Plants of India, 2, p. 606. , Tamilnadu. 1st ed. Bangalore: Cyber Media publications; Molyneux P. The use of stable free radical diphenyl picryl hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J. Sci. and technol. 2004; 26(2):212-219; Schlesier, K., Harwart, M., Bohm, V., Bitsch, R., Assessment of antioxidant activity by using different in vitro methods (2002) Free radic. Res, 30 (2), pp. 177-185; Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Antioxidant activity applying an improved ABTS radical cation decolorization assay (1998) Free Radic. Biol. Med, 72, pp. 1231-1237; Blois, Antioxidant determinations by the use of stable free radical (1958) Nature, 26, pp. 1199-1201; Benzie IFF, Strain JT. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power, the FRAP assay. Anal. Biochem. 1996; 239:70-76; Benzie, I.F.F., Szeto, Y.T., Total antioxidant capacity of teas by the ferric reducing antioxidant power assay (1999) J. Agri. Food Chem, 47, pp. 633-636; Govindrarajan, R., Vijaykumar, M., Rawat, A.K.S., Mehrotra, S., Free radical scavenging potential of Picrrorhiza kurroa Royale ex Benth (2003) Indian J.Exptl Biol, 41, pp. 875-875; Mruthunjaya, K., Hukkeri, V.I., (2008) In vitro Antioxidant and free radical scavenging potential of Parkinsonia aculeata Linn PHCOG MAG, 4 (13), pp. 42-51; Shirwaikar, A., Rajendra, K., Dinesh Kumar, C., Invitro antioxidant studies of Annona squamosa Linn, Leaves (2004) Indian J. Exptl. Biol, 42, pp. 803-807; Preito, P., Pinedo, M., Aguilar, M., Spectrophotometric quantification of antioxidant capacity through the formation of the phosphomolybdenum complex: Specific application to the determination of vitamin E (1999) Anal. Biochem, 269, pp. 337-341; Pal, D.K., Dutta, S., Evaluation of the Antioxidant activity of the roots and rhizomes of Cyperus rotundus L (2006) Indian Journal of Pharmaceutical Sciences, 68 (2), pp. 256-258; Cross, C.E., Oxygen Radical and human disease, Annals of Internal. Med (1987), 107, pp. 526-529; Marx, J.L., Oxygen free radicals linked to many diseases (1987) Science, 235, pp. 512-529; Arora, A., Sairam, R.K., Srivastava, G.C., Oxidative stress and Antioxidative system in plants (2002) Cur. Sci, 82, pp. 1227-1229; Geesin, J.G., Gordon, J.S., Berg, R.A., Retinoids affect collagen synthesis through inhibition of ascorbate induced lipid peroxidition in cultured human dermal fibroblasts (1990) Arch. Biochem. Biphy, 278, pp. 352-355; Badmis, S., Gupta, M.K., Suresh, B., Antioxidant activity of the ethanolic extract of Striga orobanchiodes (2003) J. Ethanopharmacol, 85, pp. 227-230; Sanchez-Morino, C., Method used to evaluate the free radical scavenging activity in foods and biological system (2002) Food Sci. And Technol. Int, 8, pp. 122-126; Mahakunakorn, P., Tohda, M., Murakami, Y., Matsumoto, K., Watanabe, H., Antioxidant and free radical scavenging activity of Choto-san and its related constituents (2004) Biol.Pharm. Bull, 27 (1), pp. 38-46; Chang, L.W., Yen, W.J., Huang, S.C., Duh, P.D., Antioxidant activity of Sesame coat (2002) Food Chem, 78, pp. 347-354; Halliwell, B., Reactive oxygen species in living systems: Source,biochemistry and role in human disease (1991) Am. J. Med, 91, pp. 14S-22S; Duh, P.D., Tu, Y.Y., Yen, G.C., Antioxidant acitvity of aqueous extract of harn jyur (Chrysanthemum morifolium Ramat) (1999) Lebensmittel-Wissenschaft und Technologie, 32, pp. 269-277; Gordon, M.H., The mechanism of the antioxidint action in vitro (1990) Food antioxidants, pp. 1-18. , Hudson, BJF Ed, Ekseveir, London; Rice-Evans, C., Miller, N.J., Factors affecting the antioxidant activity determined by the ABTS radical cation assay (1997) Free Radic. Res, 195, pp. 26-27; Cross, A.R., Jones, O.T.G., Enzymic mechanism of superoxide (1991) Biochem. Biophys. Acta, 387, pp. 281-285; Wiseman, H., Halliwell, B., Damage to DNA by reactive oxygen and nitrogen species: Role in in inflammatory disease and progression to cancer (1996) Biochem. J, 313, pp. 17-19; Shirwaikar, A., Prabhu, K.S., Punitha, I.S.R., In vitro antioxidant studies of Sphaeranthus indicus (Linn) (2006) Indian J. Exptl. Biol, 44, pp. 993-996

PY - 2008

Y1 - 2008

N2 - The ethanolic extract of Zornia zibbosa, is widely used in the indigenous system of medicine was studied for its in vitro scavenging activity in different methods viz. DPPH, ABTS radical scavenging, lipid peroxidation, iron chelation activity, superoxide scavenging, total antioxidant capacity and antioxidant haemoglobin glycosylation. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 14.78μg/ml for lipid peroxidation, 40.29 μg/ml for ABTS scavenging, 83.11μg/ml for iron chelating activity, 105.90μg/ml for DPPH scavenging and 97.96μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 17.96 μg/ml. In Haemaglobin glycosylation model the % scavenging was found to be 45.49% and 74.68% at the concentmtion 0.5 mg/ml and 1.0 mg/ml respectively. In all the methods the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Z. Gibbosa has significant antioxidant activity.

AB - The ethanolic extract of Zornia zibbosa, is widely used in the indigenous system of medicine was studied for its in vitro scavenging activity in different methods viz. DPPH, ABTS radical scavenging, lipid peroxidation, iron chelation activity, superoxide scavenging, total antioxidant capacity and antioxidant haemoglobin glycosylation. The results were analyzed statistically by regression method. Its antioxidant activity was estimated by IC50 value and the values are 14.78μg/ml for lipid peroxidation, 40.29 μg/ml for ABTS scavenging, 83.11μg/ml for iron chelating activity, 105.90μg/ml for DPPH scavenging and 97.96μg/ml for superoxide scavenging. Total antioxidant capacity was found to be 17.96 μg/ml. In Haemaglobin glycosylation model the % scavenging was found to be 45.49% and 74.68% at the concentmtion 0.5 mg/ml and 1.0 mg/ml respectively. In all the methods the extract showed its ability to scavenge free radicals in a concentration dependent manner. The results indicate that Z. Gibbosa has significant antioxidant activity.

M3 - Article

VL - 1

SP - 319

EP - 330

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -