Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract

Santhepete N. Manjula, Mruthunjaya Kenganora, Vipan K. Parihar, Suryakant Kumar, Pawan G. Nayak, Nitesh Kumar, Karkala Sreedhara Ranganath Pai, Chamallamudi Mallikarjuna Rao

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

In the present study, the ethanol extract of stem bark of Polyalthia longifolia Benth. and Hook (Annonaceae) was screened for its in vitro and in vivo antitumor activity. In vitro cytotoxicity of P. longifolia extract was assessed in murine cancer cells and in human cancer cells by Trypan blue exclusion assay and MTT assay, respectively. P. longifolia extract showed concentration-dependent cytotoxicity in Ehrlich's ascites carcinoma (EAC) and Dalton's ascites lymphoma (DLA) cells with IC50 values of 45.77 and 52.52 μg/mL, respectively. In the MTT assay, the IC50 values of P. longifolia extract against HeLa and MCF-7 cells were 25.24 and 50.49 μg/mL, respectively. In vivo antitumor activity against Ehrlich's ascites tumor and Dalton's solid tumor models was assessed by administering 50 and 100mg/kg of P. longifolia extract, i.p., for 7 consecutive days. P. longifolia extract, at a dose of 100mg/kg, significantly enhanced mean survival time (MST) and marginally improved hematological parameters when compared to EAC control mice. And the same dose significantly reduced the tumor volume as compared to control DLA inoculated mice. Positive control, cisplatin (3.5mg/kg, i.p., single dose), significantly enhanced MST and improved hematological parameters when compared to EAC and significantly reduced the tumor volume when compared to DLA control. In vitro antioxidant potential of P. longifolia extract was also determined owing to the role of reactive oxygen species in tumor initiation and progression. P. longifolia extract scavenged DPPH radicals, reduced ferric ions and inhibited lipid peroxidation with IC50 values of 18.14, 155.41 and 73.33 μg/mL, respectively.

Original languageEnglish
Pages (from-to)690-696
Number of pages7
JournalPharmaceutical Biology
Volume48
Issue number6
DOIs
Publication statusPublished - 2010

Fingerprint

Polyalthia
Ascites
Ethanol
Antioxidants
Inhibitory Concentration 50
Lymphoma
Tumor Burden
Carcinoma
Neoplasms
Annonaceae
Ehrlich Tumor Carcinoma
Trypan Blue
MCF-7 Cells
Cisplatin
Lipid Peroxidation
Reactive Oxygen Species
Ions

All Science Journal Classification (ASJC) codes

  • Molecular Medicine
  • Pharmacology
  • Pharmaceutical Science
  • Drug Discovery
  • Complementary and alternative medicine

Cite this

Manjula, Santhepete N. ; Kenganora, Mruthunjaya ; Parihar, Vipan K. ; Kumar, Suryakant ; Nayak, Pawan G. ; Kumar, Nitesh ; Ranganath Pai, Karkala Sreedhara ; Rao, Chamallamudi Mallikarjuna. / Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract. In: Pharmaceutical Biology. 2010 ; Vol. 48, No. 6. pp. 690-696.
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Antitumor and antioxidant activity of Polyalthia longifolia stem bark ethanol extract. / Manjula, Santhepete N.; Kenganora, Mruthunjaya; Parihar, Vipan K.; Kumar, Suryakant; Nayak, Pawan G.; Kumar, Nitesh; Ranganath Pai, Karkala Sreedhara; Rao, Chamallamudi Mallikarjuna.

In: Pharmaceutical Biology, Vol. 48, No. 6, 2010, p. 690-696.

Research output: Contribution to journalArticle

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AU - Manjula, Santhepete N.

AU - Kenganora, Mruthunjaya

AU - Parihar, Vipan K.

AU - Kumar, Suryakant

AU - Nayak, Pawan G.

AU - Kumar, Nitesh

AU - Ranganath Pai, Karkala Sreedhara

AU - Rao, Chamallamudi Mallikarjuna

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AB - In the present study, the ethanol extract of stem bark of Polyalthia longifolia Benth. and Hook (Annonaceae) was screened for its in vitro and in vivo antitumor activity. In vitro cytotoxicity of P. longifolia extract was assessed in murine cancer cells and in human cancer cells by Trypan blue exclusion assay and MTT assay, respectively. P. longifolia extract showed concentration-dependent cytotoxicity in Ehrlich's ascites carcinoma (EAC) and Dalton's ascites lymphoma (DLA) cells with IC50 values of 45.77 and 52.52 μg/mL, respectively. In the MTT assay, the IC50 values of P. longifolia extract against HeLa and MCF-7 cells were 25.24 and 50.49 μg/mL, respectively. In vivo antitumor activity against Ehrlich's ascites tumor and Dalton's solid tumor models was assessed by administering 50 and 100mg/kg of P. longifolia extract, i.p., for 7 consecutive days. P. longifolia extract, at a dose of 100mg/kg, significantly enhanced mean survival time (MST) and marginally improved hematological parameters when compared to EAC control mice. And the same dose significantly reduced the tumor volume as compared to control DLA inoculated mice. Positive control, cisplatin (3.5mg/kg, i.p., single dose), significantly enhanced MST and improved hematological parameters when compared to EAC and significantly reduced the tumor volume when compared to DLA control. In vitro antioxidant potential of P. longifolia extract was also determined owing to the role of reactive oxygen species in tumor initiation and progression. P. longifolia extract scavenged DPPH radicals, reduced ferric ions and inhibited lipid peroxidation with IC50 values of 18.14, 155.41 and 73.33 μg/mL, respectively.

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