Antitumor and radiosensitizing effects of withaferin a on mouse ehrlich ascites carcinoma in vivo

A. Chandrashekar Sharada, F. Emerson Solomon, Pathirisseri Uma Devi, Nayanabhirama Udupa, K. Kaitheri Srinivasan

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117 Citations (Scopus)

Abstract

The antitumor and radiosensitizing effects of withaferin A (WA), a steroidal lactone from Withania somnifera, was studied on Ehrlich ascites carcinoma in vivo. The acute LD50(14) for WA in Swiss mice was ~ 80 mg/kg. Twenty-four hours after i.p. inoculation of 106 tumor cells, WA was injected i.p. at different dose fractions (5 or 7.5 mg/kg x 8, 10 mg/kg x 5, 20 or 30 mg/kg x 2) with or without abdominal gamma irradiation (RT, 7.5 Gy) after the first drug dose. Increase in life span and tumor-free survival were studied up to 120 days. The drug inhibited tumor growth and increased survival, which was dependent on the WA dose per fraction rather than the total dose. Combination of RT with all the drug schedules increased tumor cure and tumor-free survival, the best effect seen after 2 fractions of 30 mg/kg each. In another experiment WA was given as 2 (40 mg/kg x 2), 3 (30 mg/kg x 3) or 4 (20 mg/kg x 4) fractions at 5, 7 or 10 days after tumor inoculation with or without RT after the first drug dose. At 7 and 10 days after inoculation the drug was effective only at 40 mg/kg x 2, but with RT 30 mg/kg x 3 produced an equal effect (20% survival) on 7 day old tumors.

Original languageEnglish
Pages (from-to)95-100
Number of pages6
JournalActa Oncologica
Volume35
Issue number1
Publication statusPublished - 1996

All Science Journal Classification (ASJC) codes

  • Oncology

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