Application of FISH-MN technique to probe micronuclei formation in normal, transformed and malignant cells using alpha satellite pan centromeric DNA

Pawan Kumar Dhar, Anna Kryscio, Christian Streffer

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A micronucleus assay employing biotinylated alpha pan centromeric DNA probe was used in the present study. The study sample consisted of 4 normal, 2 transformed and 9 malignant cell lines of human origin. The frequency of centromere positive micronuclei (c + MN) was found to decrease from normal (67%) to transformed (64.9%) to cancer cells (59%). The c + MN/c-MN ratio from normal to malignant cells was found to vary significantly (P < 0.01), while the signal ratio showed absence of any correlation (P > 0.05). These results demonstrate the usefulness of combining FISH technique and micronuclei assay to trace the mechanism of micronucleus formation in cells of diverse histopathological origin.

Original languageEnglish
Pages (from-to)1247-1249
Number of pages3
JournalCurrent Science
Volume78
Issue number10
Publication statusPublished - 25-05-2000
Externally publishedYes

Fingerprint

Fluorescence In Situ Hybridization
Micronucleus Tests
DNA
Centromere
DNA Probes
Cell Line
Neoplasms

All Science Journal Classification (ASJC) codes

  • General

Cite this

@article{bfe1cb0439744b6c80a069a4cad59ad4,
title = "Application of FISH-MN technique to probe micronuclei formation in normal, transformed and malignant cells using alpha satellite pan centromeric DNA",
abstract = "A micronucleus assay employing biotinylated alpha pan centromeric DNA probe was used in the present study. The study sample consisted of 4 normal, 2 transformed and 9 malignant cell lines of human origin. The frequency of centromere positive micronuclei (c + MN) was found to decrease from normal (67{\%}) to transformed (64.9{\%}) to cancer cells (59{\%}). The c + MN/c-MN ratio from normal to malignant cells was found to vary significantly (P < 0.01), while the signal ratio showed absence of any correlation (P > 0.05). These results demonstrate the usefulness of combining FISH technique and micronuclei assay to trace the mechanism of micronucleus formation in cells of diverse histopathological origin.",
author = "Dhar, {Pawan Kumar} and Anna Kryscio and Christian Streffer",
year = "2000",
month = "5",
day = "25",
language = "English",
volume = "78",
pages = "1247--1249",
journal = "Current Science",
issn = "0011-3891",
publisher = "Indian Academy of Sciences",
number = "10",

}

Application of FISH-MN technique to probe micronuclei formation in normal, transformed and malignant cells using alpha satellite pan centromeric DNA. / Dhar, Pawan Kumar; Kryscio, Anna; Streffer, Christian.

In: Current Science, Vol. 78, No. 10, 25.05.2000, p. 1247-1249.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Application of FISH-MN technique to probe micronuclei formation in normal, transformed and malignant cells using alpha satellite pan centromeric DNA

AU - Dhar, Pawan Kumar

AU - Kryscio, Anna

AU - Streffer, Christian

PY - 2000/5/25

Y1 - 2000/5/25

N2 - A micronucleus assay employing biotinylated alpha pan centromeric DNA probe was used in the present study. The study sample consisted of 4 normal, 2 transformed and 9 malignant cell lines of human origin. The frequency of centromere positive micronuclei (c + MN) was found to decrease from normal (67%) to transformed (64.9%) to cancer cells (59%). The c + MN/c-MN ratio from normal to malignant cells was found to vary significantly (P < 0.01), while the signal ratio showed absence of any correlation (P > 0.05). These results demonstrate the usefulness of combining FISH technique and micronuclei assay to trace the mechanism of micronucleus formation in cells of diverse histopathological origin.

AB - A micronucleus assay employing biotinylated alpha pan centromeric DNA probe was used in the present study. The study sample consisted of 4 normal, 2 transformed and 9 malignant cell lines of human origin. The frequency of centromere positive micronuclei (c + MN) was found to decrease from normal (67%) to transformed (64.9%) to cancer cells (59%). The c + MN/c-MN ratio from normal to malignant cells was found to vary significantly (P < 0.01), while the signal ratio showed absence of any correlation (P > 0.05). These results demonstrate the usefulness of combining FISH technique and micronuclei assay to trace the mechanism of micronucleus formation in cells of diverse histopathological origin.

UR - http://www.scopus.com/inward/record.url?scp=0000997308&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0000997308&partnerID=8YFLogxK

M3 - Article

VL - 78

SP - 1247

EP - 1249

JO - Current Science

JF - Current Science

SN - 0011-3891

IS - 10

ER -