Application of HPLC combined with laser induced fluorescence for protein profile analysis of tissue homogenates in cervical cancer

Sujatha Bhat, Ajeetkumar Patil, Lavanya Rai, V. B. Kartha, Santhosh Chidangil

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

A highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patients and the patients suffering from different stages of the cervical cancer were recorded by HPLC-LIF and analysed by Principle Component Analysis (PCA) to get statistical information on different tissue components. Discrimination of different stages of the samples was carried out by considering three parametersscores of factor, spectral residual, and Mahalanobis Distance. Diagnostic accuracy of the method was evaluated using Receiver Operating Characteristic (ROC) analysis, and Youden's index (J) plots. The PCA results showed high sensitivity and specificity (100) for cervical cancer diagnosis. ROC and Youden's index curves for both normal and malignant standard sets show good diagnostic accuracy with high AUC values. The statistical analysis has shown that the differences in protein profiles can be used to diagnose biochemical changes in the tissue, and thus can be readily applied for the detection of cervical cancer, even in situations where a histopathology examination is not easy because of nonavailability of experienced pathologists.

Original languageEnglish
Article number976421
JournalThe Scientific World Journal
Volume2012
DOIs
Publication statusPublished - 21-06-2012

Fingerprint

laser induced fluorescence
Uterine Cervical Neoplasms
cancer
Lasers
Fluorescence
High Pressure Liquid Chromatography
High performance liquid chromatography
Tissue
protein
ROC Curve
liquid chromatography
Proteins
Biopsy
histopathology
Statistical methods
Hysterectomy
Area Under Curve
statistical analysis
Sensitivity and Specificity
analysis

All Science Journal Classification (ASJC) codes

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)

Cite this

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abstract = "A highly objective method, High Performance Liquid Chromatography with Laser Induced Fluorescence (HPLC-LIF) technique was used to study the protein profiles of normal and cervical cancer tissue homogenates. A total of 44 samples including normal cervical biopsy samples from the hysterectomy patients and the patients suffering from different stages of the cervical cancer were recorded by HPLC-LIF and analysed by Principle Component Analysis (PCA) to get statistical information on different tissue components. Discrimination of different stages of the samples was carried out by considering three parametersscores of factor, spectral residual, and Mahalanobis Distance. Diagnostic accuracy of the method was evaluated using Receiver Operating Characteristic (ROC) analysis, and Youden's index (J) plots. The PCA results showed high sensitivity and specificity (100) for cervical cancer diagnosis. ROC and Youden's index curves for both normal and malignant standard sets show good diagnostic accuracy with high AUC values. The statistical analysis has shown that the differences in protein profiles can be used to diagnose biochemical changes in the tissue, and thus can be readily applied for the detection of cervical cancer, even in situations where a histopathology examination is not easy because of nonavailability of experienced pathologists.",
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Application of HPLC combined with laser induced fluorescence for protein profile analysis of tissue homogenates in cervical cancer. / Bhat, Sujatha; Patil, Ajeetkumar; Rai, Lavanya; Kartha, V. B.; Chidangil, Santhosh.

In: The Scientific World Journal, Vol. 2012, 976421, 21.06.2012.

Research output: Contribution to journalArticle

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