Valoración y comparación de los cambios nucleares observados en gingivitis y periodontitis, mediante microscopio fluorescente

Translated title of the contribution: Assessment and comparison of nuclear changes seen in gingivitis and periodontitis using fluorescent microscopy

Tanya Sawhney, Nidhi Manaktala, Swati Pralhad, Srikant Natarajan

Research output: Contribution to journalArticle

Abstract

Introduction and objectives: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. Methods and material: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. Results and conclusion: A statistically significant difference was observed when the age of the 3 groups were compared (p = 0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p = 0.002) and multinucleation (p < 0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis.

Original languageSpanish
JournalRevista Espanola de Patologia
DOIs
Publication statusPublished - 01-01-2019

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Gingivitis
Periodontitis
Microscopy
Chronic Periodontitis
Nuclear Microscopy
Control Groups
Acridine Orange
Gingiva
Analysis of Variance
Healthy Volunteers
Coloring Agents
Epithelium
Age Groups

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine

Cite this

@article{29080d0d91974aad96a273670ad77938,
title = "Valoraci{\'o}n y comparaci{\'o}n de los cambios nucleares observados en gingivitis y periodontitis, mediante microscopio fluorescente",
abstract = "Introduction and objectives: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. Methods and material: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. Results and conclusion: A statistically significant difference was observed when the age of the 3 groups were compared (p = 0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p = 0.002) and multinucleation (p < 0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis.",
author = "Tanya Sawhney and Nidhi Manaktala and Swati Pralhad and Srikant Natarajan",
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doi = "10.1016/j.patol.2019.03.002",
language = "Spanish",
journal = "Revista Espanola de Patologia",
issn = "1699-8855",
publisher = "Ediciones Doyma, S.L.",

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Valoración y comparación de los cambios nucleares observados en gingivitis y periodontitis, mediante microscopio fluorescente. / Sawhney, Tanya; Manaktala, Nidhi; Pralhad, Swati; Natarajan, Srikant.

In: Revista Espanola de Patologia, 01.01.2019.

Research output: Contribution to journalArticle

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T1 - Valoración y comparación de los cambios nucleares observados en gingivitis y periodontitis, mediante microscopio fluorescente

AU - Sawhney, Tanya

AU - Manaktala, Nidhi

AU - Pralhad, Swati

AU - Natarajan, Srikant

PY - 2019/1/1

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N2 - Introduction and objectives: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. Methods and material: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. Results and conclusion: A statistically significant difference was observed when the age of the 3 groups were compared (p = 0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p = 0.002) and multinucleation (p < 0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis.

AB - Introduction and objectives: The objective of this study was to observe the evolution of genotoxicity (micronucleation, binucleation and multinucleation) from normal to periodontally compromised gingival epithelium (gingivitis and periodontitis) and to compare the severity of damage. Methods and material: 45 participants formed 3 different categories; a control group of 15 healthy subjects, 15 subjects with gingivitis and 15 with chronic periodontitis. Smears were collected from all the gingiva and stained with acridine orange stain. A total of 500 cells were evaluated under fluorescent microscopy for nuclear abnormalities such as micronuclei, binucleation and multinucleation. The statistical analysis used was one way ANOVA and posthoc Tukey test. Results and conclusion: A statistically significant difference was observed when the age of the 3 groups were compared (p = 0.002); the control group were younger than those with chronic periodontitis or gingivitis. With respect to genotoxic changes, the differences for binucleation (p = 0.002) and multinucleation (p < 0.001) were statistically significant thus suggesting advanced damage in the nucleus. Such changes in genotoxicity could be of help to a clinician in determining prognosis.

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