Spermatozoada dna hasari{dotless}ni{dotless}n boyutu ile fertilizasyon ve implantasyon öncesi embriyolari{dotless}n gelişimsel kompetansi{dotless} arasi{dotless}ndaki ilişki

Translated title of the contribution: Association between the extent of DNA damage in the spermatozoa, fertilization and developmental competence in preimplantation stage embryos

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objective: To examine the fertilizing ability and DNA damage response of preimplantation stage embryos derived from the γ-irradiated mouse sperm carrying varying amounts of DNA strand-breaks. Material and Methods: The DNA damage in the sperm was induced by exposing the testicular area to different doses of γ-radiation. After mating with healthy female mice, sperm zona binding, fertilizing ability of DNA damaged sperm and developmental competence of embryos derived from the DNA damaged sperm were assessed. Results: The in vivo zona binding ability and fertilizing ability of DNA damaged sperm was significantly affected in the 5.0 and 10.0 Gy sperm irradiation groups. Although the development of the embryos derived from the DNA damaged sperm was not significantly affected until day 2.5 post-coitus, further development was significantly altered, as evidenced by the total cell number in the embryos. Conclusion: The sperm carrying DNA strand breaks still has the ability to fertilize the oocyte normally. However, the events like zona-binding and successful fertilization depend on the extent of sperm DNA fragmentation. The study has also showed a great heterogeneity in embryonic development at peri-implantation period with respect to the degree of sperm DNA damage.

Original languageTurkish
Pages (from-to)182-186
Number of pages5
JournalJournal of the Turkish German Gynecology Association
Volume11
Issue number4
DOIs
Publication statusPublished - 2010

Fingerprint

Blastocyst
Fertilization
Mental Competency
DNA Damage
Spermatozoa
Aptitude
Herpes Zoster
DNA Breaks
DNA
Embryonic Development
Embryonic Structures
Coitus
DNA Fragmentation
Oocytes
Cell Count
Radiation

All Science Journal Classification (ASJC) codes

  • Obstetrics and Gynaecology

Cite this

@article{b0fba3ecb7944c9a9847dc6b9117829e,
title = "Spermatozoada dna hasari{dotless}ni{dotless}n boyutu ile fertilizasyon ve implantasyon {\"o}ncesi embriyolari{dotless}n gelişimsel kompetansi{dotless} arasi{dotless}ndaki ilişki",
abstract = "Objective: To examine the fertilizing ability and DNA damage response of preimplantation stage embryos derived from the γ-irradiated mouse sperm carrying varying amounts of DNA strand-breaks. Material and Methods: The DNA damage in the sperm was induced by exposing the testicular area to different doses of γ-radiation. After mating with healthy female mice, sperm zona binding, fertilizing ability of DNA damaged sperm and developmental competence of embryos derived from the DNA damaged sperm were assessed. Results: The in vivo zona binding ability and fertilizing ability of DNA damaged sperm was significantly affected in the 5.0 and 10.0 Gy sperm irradiation groups. Although the development of the embryos derived from the DNA damaged sperm was not significantly affected until day 2.5 post-coitus, further development was significantly altered, as evidenced by the total cell number in the embryos. Conclusion: The sperm carrying DNA strand breaks still has the ability to fertilize the oocyte normally. However, the events like zona-binding and successful fertilization depend on the extent of sperm DNA fragmentation. The study has also showed a great heterogeneity in embryonic development at peri-implantation period with respect to the degree of sperm DNA damage.",
author = "Dinesh Upadhya and Guruprasad Kalthur and Pratap Kumar and Rao, {Bola S.} and Adiga, {Satish K.}",
year = "2010",
doi = "0.5152/jtgga.2010.3",
language = "Turkish",
volume = "11",
pages = "182--186",
journal = "Journal of the Turkish German Gynecology Association",
issn = "1309-0399",
publisher = "Aves",
number = "4",

}

TY - JOUR

T1 - Spermatozoada dna hasari{dotless}ni{dotless}n boyutu ile fertilizasyon ve implantasyon öncesi embriyolari{dotless}n gelişimsel kompetansi{dotless} arasi{dotless}ndaki ilişki

AU - Upadhya, Dinesh

AU - Kalthur, Guruprasad

AU - Kumar, Pratap

AU - Rao, Bola S.

AU - Adiga, Satish K.

PY - 2010

Y1 - 2010

N2 - Objective: To examine the fertilizing ability and DNA damage response of preimplantation stage embryos derived from the γ-irradiated mouse sperm carrying varying amounts of DNA strand-breaks. Material and Methods: The DNA damage in the sperm was induced by exposing the testicular area to different doses of γ-radiation. After mating with healthy female mice, sperm zona binding, fertilizing ability of DNA damaged sperm and developmental competence of embryos derived from the DNA damaged sperm were assessed. Results: The in vivo zona binding ability and fertilizing ability of DNA damaged sperm was significantly affected in the 5.0 and 10.0 Gy sperm irradiation groups. Although the development of the embryos derived from the DNA damaged sperm was not significantly affected until day 2.5 post-coitus, further development was significantly altered, as evidenced by the total cell number in the embryos. Conclusion: The sperm carrying DNA strand breaks still has the ability to fertilize the oocyte normally. However, the events like zona-binding and successful fertilization depend on the extent of sperm DNA fragmentation. The study has also showed a great heterogeneity in embryonic development at peri-implantation period with respect to the degree of sperm DNA damage.

AB - Objective: To examine the fertilizing ability and DNA damage response of preimplantation stage embryos derived from the γ-irradiated mouse sperm carrying varying amounts of DNA strand-breaks. Material and Methods: The DNA damage in the sperm was induced by exposing the testicular area to different doses of γ-radiation. After mating with healthy female mice, sperm zona binding, fertilizing ability of DNA damaged sperm and developmental competence of embryos derived from the DNA damaged sperm were assessed. Results: The in vivo zona binding ability and fertilizing ability of DNA damaged sperm was significantly affected in the 5.0 and 10.0 Gy sperm irradiation groups. Although the development of the embryos derived from the DNA damaged sperm was not significantly affected until day 2.5 post-coitus, further development was significantly altered, as evidenced by the total cell number in the embryos. Conclusion: The sperm carrying DNA strand breaks still has the ability to fertilize the oocyte normally. However, the events like zona-binding and successful fertilization depend on the extent of sperm DNA fragmentation. The study has also showed a great heterogeneity in embryonic development at peri-implantation period with respect to the degree of sperm DNA damage.

UR - http://www.scopus.com/inward/record.url?scp=79251562889&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79251562889&partnerID=8YFLogxK

U2 - 0.5152/jtgga.2010.3

DO - 0.5152/jtgga.2010.3

M3 - Article

AN - SCOPUS:79251562889

VL - 11

SP - 182

EP - 186

JO - Journal of the Turkish German Gynecology Association

JF - Journal of the Turkish German Gynecology Association

SN - 1309-0399

IS - 4

ER -