Association of HSD11B1 gene polymorphisms with type 2 diabetes and metabolic syndrome in South Indian population

Nayana Devang, Kapaettu Satyamoorthy, Padmalatha S. Rai, M. Nandini, Satish Rao, Nagaraja M. Phani, Prabha Adhikari

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Background 11beta-hydroxysteroid dehydrogenase Type 1 (11β-HSD1) is an NADP or NADPH-dependent enzyme that generates cortisol from cortisone for a local glucocorticoid action. Functional polymorphisms within 11beta-hydroxysteroid dehydrogenase Type 1 (HSD11B1) gene have shown an association with various factors, including insulin resistance (IR) and hypertension. In our study, we have assessed the association of HSD11B1 (rs12086634 and rs846910) gene polymorphisms with type 2 diabetes (T2D) and metabolic syndrome (metS). Methods In the present study, 616 subjects were enrolled. DNA from T2D subjects (n = 207), metS subjects (n = 101), and their age and sex matched control subjects were analyzed. Genotyping of HSD11B1 rs12086634 and rs846910 single nucleotide polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). An odds ratio and 95% confidence interval were calculated to determine the association of HSD11B1 gene polymorphisms with T2D and metS. Results The association analysis indicated that HSD11B1 rs12086634 TG contributed to an increased risk of both T2D (OR = 1.91; 95% CI-1.33-2.76, P = 0.0005) and metS (OR = 2.37; 95% CI-1.39-4.05, P = 0.0015), but HSD11B1 rs846910 AG contributed to an increased risk of T2D (OR = 1.62; 95% CI-1.02-2.57, P = 0.03) only. There was a statistically significant difference in systolic blood pressure between the control group with HSD11B1 rs12086634 TG genotype (128.96 ± 13.19 mmHg) and the control group with HSD11B1 rs12086634 TT genotype (123.27 ± 10.84 mmHg). Conclusions The results of our study indicated that the HSD11B1 rs12086634 is associated with both T2D and metS, but HSD11B1 rs846910 is associated with only T2D in South Indian population.

Original languageEnglish
Pages (from-to)142-148
Number of pages7
JournalDiabetes Research and Clinical Practice
Volume131
DOIs
Publication statusPublished - 01-09-2017

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Type 2 Diabetes Mellitus
Population
Genes
11-beta-Hydroxysteroid Dehydrogenase Type 1
NADP
Genotype
Blood Pressure
Control Groups
Cortisone
Glucocorticoids
Single Nucleotide Polymorphism
Insulin Resistance
Hydrocortisone
Odds Ratio
Confidence Intervals
Hypertension
Polymerase Chain Reaction
Mutation
DNA
Enzymes

All Science Journal Classification (ASJC) codes

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

@article{4270808de0324352914f552a6aed7b95,
title = "Association of HSD11B1 gene polymorphisms with type 2 diabetes and metabolic syndrome in South Indian population",
abstract = "Background 11beta-hydroxysteroid dehydrogenase Type 1 (11β-HSD1) is an NADP or NADPH-dependent enzyme that generates cortisol from cortisone for a local glucocorticoid action. Functional polymorphisms within 11beta-hydroxysteroid dehydrogenase Type 1 (HSD11B1) gene have shown an association with various factors, including insulin resistance (IR) and hypertension. In our study, we have assessed the association of HSD11B1 (rs12086634 and rs846910) gene polymorphisms with type 2 diabetes (T2D) and metabolic syndrome (metS). Methods In the present study, 616 subjects were enrolled. DNA from T2D subjects (n = 207), metS subjects (n = 101), and their age and sex matched control subjects were analyzed. Genotyping of HSD11B1 rs12086634 and rs846910 single nucleotide polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). An odds ratio and 95{\%} confidence interval were calculated to determine the association of HSD11B1 gene polymorphisms with T2D and metS. Results The association analysis indicated that HSD11B1 rs12086634 TG contributed to an increased risk of both T2D (OR = 1.91; 95{\%} CI-1.33-2.76, P = 0.0005) and metS (OR = 2.37; 95{\%} CI-1.39-4.05, P = 0.0015), but HSD11B1 rs846910 AG contributed to an increased risk of T2D (OR = 1.62; 95{\%} CI-1.02-2.57, P = 0.03) only. There was a statistically significant difference in systolic blood pressure between the control group with HSD11B1 rs12086634 TG genotype (128.96 ± 13.19 mmHg) and the control group with HSD11B1 rs12086634 TT genotype (123.27 ± 10.84 mmHg). Conclusions The results of our study indicated that the HSD11B1 rs12086634 is associated with both T2D and metS, but HSD11B1 rs846910 is associated with only T2D in South Indian population.",
author = "Nayana Devang and Kapaettu Satyamoorthy and Rai, {Padmalatha S.} and M. Nandini and Satish Rao and Phani, {Nagaraja M.} and Prabha Adhikari",
year = "2017",
month = "9",
day = "1",
doi = "10.1016/j.diabres.2017.07.011",
language = "English",
volume = "131",
pages = "142--148",
journal = "Diabetes Research and Clinical Practice",
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publisher = "Elsevier Ireland Ltd",

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Association of HSD11B1 gene polymorphisms with type 2 diabetes and metabolic syndrome in South Indian population. / Devang, Nayana; Satyamoorthy, Kapaettu; Rai, Padmalatha S.; Nandini, M.; Rao, Satish; Phani, Nagaraja M.; Adhikari, Prabha.

In: Diabetes Research and Clinical Practice, Vol. 131, 01.09.2017, p. 142-148.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Association of HSD11B1 gene polymorphisms with type 2 diabetes and metabolic syndrome in South Indian population

AU - Devang, Nayana

AU - Satyamoorthy, Kapaettu

AU - Rai, Padmalatha S.

AU - Nandini, M.

AU - Rao, Satish

AU - Phani, Nagaraja M.

AU - Adhikari, Prabha

PY - 2017/9/1

Y1 - 2017/9/1

N2 - Background 11beta-hydroxysteroid dehydrogenase Type 1 (11β-HSD1) is an NADP or NADPH-dependent enzyme that generates cortisol from cortisone for a local glucocorticoid action. Functional polymorphisms within 11beta-hydroxysteroid dehydrogenase Type 1 (HSD11B1) gene have shown an association with various factors, including insulin resistance (IR) and hypertension. In our study, we have assessed the association of HSD11B1 (rs12086634 and rs846910) gene polymorphisms with type 2 diabetes (T2D) and metabolic syndrome (metS). Methods In the present study, 616 subjects were enrolled. DNA from T2D subjects (n = 207), metS subjects (n = 101), and their age and sex matched control subjects were analyzed. Genotyping of HSD11B1 rs12086634 and rs846910 single nucleotide polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). An odds ratio and 95% confidence interval were calculated to determine the association of HSD11B1 gene polymorphisms with T2D and metS. Results The association analysis indicated that HSD11B1 rs12086634 TG contributed to an increased risk of both T2D (OR = 1.91; 95% CI-1.33-2.76, P = 0.0005) and metS (OR = 2.37; 95% CI-1.39-4.05, P = 0.0015), but HSD11B1 rs846910 AG contributed to an increased risk of T2D (OR = 1.62; 95% CI-1.02-2.57, P = 0.03) only. There was a statistically significant difference in systolic blood pressure between the control group with HSD11B1 rs12086634 TG genotype (128.96 ± 13.19 mmHg) and the control group with HSD11B1 rs12086634 TT genotype (123.27 ± 10.84 mmHg). Conclusions The results of our study indicated that the HSD11B1 rs12086634 is associated with both T2D and metS, but HSD11B1 rs846910 is associated with only T2D in South Indian population.

AB - Background 11beta-hydroxysteroid dehydrogenase Type 1 (11β-HSD1) is an NADP or NADPH-dependent enzyme that generates cortisol from cortisone for a local glucocorticoid action. Functional polymorphisms within 11beta-hydroxysteroid dehydrogenase Type 1 (HSD11B1) gene have shown an association with various factors, including insulin resistance (IR) and hypertension. In our study, we have assessed the association of HSD11B1 (rs12086634 and rs846910) gene polymorphisms with type 2 diabetes (T2D) and metabolic syndrome (metS). Methods In the present study, 616 subjects were enrolled. DNA from T2D subjects (n = 207), metS subjects (n = 101), and their age and sex matched control subjects were analyzed. Genotyping of HSD11B1 rs12086634 and rs846910 single nucleotide polymorphisms was performed using tetra-primer amplification refractory mutation system-polymerase chain reaction (T-ARMS-PCR). An odds ratio and 95% confidence interval were calculated to determine the association of HSD11B1 gene polymorphisms with T2D and metS. Results The association analysis indicated that HSD11B1 rs12086634 TG contributed to an increased risk of both T2D (OR = 1.91; 95% CI-1.33-2.76, P = 0.0005) and metS (OR = 2.37; 95% CI-1.39-4.05, P = 0.0015), but HSD11B1 rs846910 AG contributed to an increased risk of T2D (OR = 1.62; 95% CI-1.02-2.57, P = 0.03) only. There was a statistically significant difference in systolic blood pressure between the control group with HSD11B1 rs12086634 TG genotype (128.96 ± 13.19 mmHg) and the control group with HSD11B1 rs12086634 TT genotype (123.27 ± 10.84 mmHg). Conclusions The results of our study indicated that the HSD11B1 rs12086634 is associated with both T2D and metS, but HSD11B1 rs846910 is associated with only T2D in South Indian population.

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U2 - 10.1016/j.diabres.2017.07.011

DO - 10.1016/j.diabres.2017.07.011

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AN - SCOPUS:85025159043

VL - 131

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EP - 148

JO - Diabetes Research and Clinical Practice

JF - Diabetes Research and Clinical Practice

SN - 0168-8227

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