Autofluorescence of oral tissue for optical pathology in oral malignancy

B. K. Manjunath, J. Kurein, L. Rao, C. Murali Krishna, M. S. Chidananda, K. Venkatakrishna, V. B. Kartha

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

Pulsed laser-induced-fluorescence studies of pathologically certified oral tissues are carried out at different excitations and time delays. Among the several excitations used, 325 nm produced noticeably different spectral profile for normal and malignant tissues. Extensive curve analysis was carried out in order to understand changes in biochemical composition of tissue based on spectral profiles. Curve resolution and principal component analysis (PCA) show that the fluorescence intensity changes from normal to malignant tissue samples are not completely explained in terms of simple collagen and NAD(P)H intensity changes. The spectra require atleast five components to be fully accounted for. Several discrimination methodologies based on PCA and intensity differences between different emission peaks (resultant peaks of curve analysis) were also evaluated. The results obtained indicate PCA using Mahalanobis distance and spectral residual as discrimination parameters provides best discrimination and can be used for matching unknown samples to standard calibration sets. Intensity ratio of bound NAD(P)H to collagen seems to be more suitable for discrimination between normal and malignant oral tissue, compared to ratio of collagen to total intensity of all the other components together.

Original languageEnglish
Pages (from-to)49-58
Number of pages10
JournalJournal of Photochemistry and Photobiology B: Biology
Volume73
Issue number1-2
DOIs
Publication statusPublished - 23-01-2004
Externally publishedYes

Fingerprint

Oral Pathology
pathology
discrimination
collagens
principal components analysis
Principal Component Analysis
Collagen
Neoplasms
NAD
curves
Fluorescence
profiles
laser induced fluorescence
Calibration
excitation
pulsed lasers
Lasers
time lag
methodology
fluorescence

All Science Journal Classification (ASJC) codes

  • Radiation
  • Radiological and Ultrasound Technology
  • Biophysics
  • Radiology Nuclear Medicine and imaging

Cite this

Manjunath, B. K., Kurein, J., Rao, L., Murali Krishna, C., Chidananda, M. S., Venkatakrishna, K., & Kartha, V. B. (2004). Autofluorescence of oral tissue for optical pathology in oral malignancy. Journal of Photochemistry and Photobiology B: Biology, 73(1-2), 49-58. https://doi.org/10.1016/j.jphotobiol.2003.09.004
Manjunath, B. K. ; Kurein, J. ; Rao, L. ; Murali Krishna, C. ; Chidananda, M. S. ; Venkatakrishna, K. ; Kartha, V. B. / Autofluorescence of oral tissue for optical pathology in oral malignancy. In: Journal of Photochemistry and Photobiology B: Biology. 2004 ; Vol. 73, No. 1-2. pp. 49-58.
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Manjunath, BK, Kurein, J, Rao, L, Murali Krishna, C, Chidananda, MS, Venkatakrishna, K & Kartha, VB 2004, 'Autofluorescence of oral tissue for optical pathology in oral malignancy', Journal of Photochemistry and Photobiology B: Biology, vol. 73, no. 1-2, pp. 49-58. https://doi.org/10.1016/j.jphotobiol.2003.09.004

Autofluorescence of oral tissue for optical pathology in oral malignancy. / Manjunath, B. K.; Kurein, J.; Rao, L.; Murali Krishna, C.; Chidananda, M. S.; Venkatakrishna, K.; Kartha, V. B.

In: Journal of Photochemistry and Photobiology B: Biology, Vol. 73, No. 1-2, 23.01.2004, p. 49-58.

Research output: Contribution to journalArticle

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Manjunath BK, Kurein J, Rao L, Murali Krishna C, Chidananda MS, Venkatakrishna K et al. Autofluorescence of oral tissue for optical pathology in oral malignancy. Journal of Photochemistry and Photobiology B: Biology. 2004 Jan 23;73(1-2):49-58. https://doi.org/10.1016/j.jphotobiol.2003.09.004