Abstract

Objective: The objective of this study was to evaluate the efficacy of laser-induced fluorescence (LIF) in combination with principal component analysis (PCA) to characterize osteoporotic conditions induced by ovariectomy (OVX) in mice. Background Data: The dual energy X-ray absorptiometry is the gold standard methodology routinely used to diagnose osteoporosis. In recent years, the use of LIF to characterize human disease and to aid in diagnosis has shown great promise. However, this technique has not been much exploited for monitoring osteoporosis. Materials and Methods: Swiss albino mice were sacrificed 2, 3, 4, and 5wk after OVX and their femur bones were excised. The same protocol was used for age-matched female controls (no OVX). The LIF spectra from different regions of the bones were recorded and compared using PCA. Results: A significant change in the fluorescence pattern of osteoporotic bones compared with the control was indicated by PCA match/no-match analysis. A region-wise PCA match/no-match analysis of the spectral changes against respective region calibration sets indicated more no-matches in the fifth week bones compared with the others. Further, the spectral differences were more prominent in the proximal and distal parts of the bones. In addition to the PCA, Gaussian curve fitting was also performed on control, third week, and fifth week bone spectra to identify different spectral components in them. Conclusion: This preliminary study using fluorescence spectroscopy in combination with PCA clearly demonstrated osteoporotic changes in mouse femur bones at different time points after OVX, suggesting possible human applications.

Original languageEnglish
Pages (from-to)227-232
Number of pages6
JournalPhotomedicine and Laser Surgery
Volume29
Issue number4
DOIs
Publication statusPublished - 01-04-2011

Fingerprint

Femur
Principal Component Analysis
Bone
Principal component analysis
Bone and Bones
Fluorescence
Lasers
Osteoporosis
Fluorescence Spectrometry
Photon Absorptiometry
Fluorescence spectroscopy
Curve fitting
Ovariectomy
Calibration
X rays
Monitoring

All Science Journal Classification (ASJC) codes

  • Biomedical Engineering
  • Radiology Nuclear Medicine and imaging

Cite this

@article{6e7d38825c6e4b03b7af3f6d176f5168,
title = "Autofluorescence of osteoporotic mouse femur bones: A pilot study",
abstract = "Objective: The objective of this study was to evaluate the efficacy of laser-induced fluorescence (LIF) in combination with principal component analysis (PCA) to characterize osteoporotic conditions induced by ovariectomy (OVX) in mice. Background Data: The dual energy X-ray absorptiometry is the gold standard methodology routinely used to diagnose osteoporosis. In recent years, the use of LIF to characterize human disease and to aid in diagnosis has shown great promise. However, this technique has not been much exploited for monitoring osteoporosis. Materials and Methods: Swiss albino mice were sacrificed 2, 3, 4, and 5wk after OVX and their femur bones were excised. The same protocol was used for age-matched female controls (no OVX). The LIF spectra from different regions of the bones were recorded and compared using PCA. Results: A significant change in the fluorescence pattern of osteoporotic bones compared with the control was indicated by PCA match/no-match analysis. A region-wise PCA match/no-match analysis of the spectral changes against respective region calibration sets indicated more no-matches in the fifth week bones compared with the others. Further, the spectral differences were more prominent in the proximal and distal parts of the bones. In addition to the PCA, Gaussian curve fitting was also performed on control, third week, and fifth week bone spectra to identify different spectral components in them. Conclusion: This preliminary study using fluorescence spectroscopy in combination with PCA clearly demonstrated osteoporotic changes in mouse femur bones at different time points after OVX, suggesting possible human applications.",
author = "Pratyush Kumar and Satish Rao and Kamath, {Sudha D.} and Vijendra Prabhu and K. Satyamoorthy and Mahato, {K. K.}",
year = "2011",
month = "4",
day = "1",
doi = "10.1089/pho.2009.2725",
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T1 - Autofluorescence of osteoporotic mouse femur bones

T2 - A pilot study

AU - Kumar, Pratyush

AU - Rao, Satish

AU - Kamath, Sudha D.

AU - Prabhu, Vijendra

AU - Satyamoorthy, K.

AU - Mahato, K. K.

PY - 2011/4/1

Y1 - 2011/4/1

N2 - Objective: The objective of this study was to evaluate the efficacy of laser-induced fluorescence (LIF) in combination with principal component analysis (PCA) to characterize osteoporotic conditions induced by ovariectomy (OVX) in mice. Background Data: The dual energy X-ray absorptiometry is the gold standard methodology routinely used to diagnose osteoporosis. In recent years, the use of LIF to characterize human disease and to aid in diagnosis has shown great promise. However, this technique has not been much exploited for monitoring osteoporosis. Materials and Methods: Swiss albino mice were sacrificed 2, 3, 4, and 5wk after OVX and their femur bones were excised. The same protocol was used for age-matched female controls (no OVX). The LIF spectra from different regions of the bones were recorded and compared using PCA. Results: A significant change in the fluorescence pattern of osteoporotic bones compared with the control was indicated by PCA match/no-match analysis. A region-wise PCA match/no-match analysis of the spectral changes against respective region calibration sets indicated more no-matches in the fifth week bones compared with the others. Further, the spectral differences were more prominent in the proximal and distal parts of the bones. In addition to the PCA, Gaussian curve fitting was also performed on control, third week, and fifth week bone spectra to identify different spectral components in them. Conclusion: This preliminary study using fluorescence spectroscopy in combination with PCA clearly demonstrated osteoporotic changes in mouse femur bones at different time points after OVX, suggesting possible human applications.

AB - Objective: The objective of this study was to evaluate the efficacy of laser-induced fluorescence (LIF) in combination with principal component analysis (PCA) to characterize osteoporotic conditions induced by ovariectomy (OVX) in mice. Background Data: The dual energy X-ray absorptiometry is the gold standard methodology routinely used to diagnose osteoporosis. In recent years, the use of LIF to characterize human disease and to aid in diagnosis has shown great promise. However, this technique has not been much exploited for monitoring osteoporosis. Materials and Methods: Swiss albino mice were sacrificed 2, 3, 4, and 5wk after OVX and their femur bones were excised. The same protocol was used for age-matched female controls (no OVX). The LIF spectra from different regions of the bones were recorded and compared using PCA. Results: A significant change in the fluorescence pattern of osteoporotic bones compared with the control was indicated by PCA match/no-match analysis. A region-wise PCA match/no-match analysis of the spectral changes against respective region calibration sets indicated more no-matches in the fifth week bones compared with the others. Further, the spectral differences were more prominent in the proximal and distal parts of the bones. In addition to the PCA, Gaussian curve fitting was also performed on control, third week, and fifth week bone spectra to identify different spectral components in them. Conclusion: This preliminary study using fluorescence spectroscopy in combination with PCA clearly demonstrated osteoporotic changes in mouse femur bones at different time points after OVX, suggesting possible human applications.

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