Azadirachta indica exhibits chemopreventive action against hepatic cancer: Studies on associated histopathological and ultrastructural changes

Sanjay Bharati, Praveen Rishi, Ashwani Koul

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

The present study was designed to evaluate the anticarcinogenic potential of Azadirachta indica against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis. Further, the associated histopathological and ultrastructural changes were also analyzed. Hepatic cancer model was developed by the intraperitoneal administration of NDEA to mice at weekly intervals, in successive increasing doses, for a period of 8 weeks. Aqueous A. indica leaf extract (AAILE) was administered orally at a dosage of 100 μg/g body weight thrice a week till termination of the study. A relationship between histopathological grading and chemopreventive effect of A. indica had been established at various stages of carcinogenesis. Anticancer activity of A. indica was evaluated in terms of tumor incidence, tumor multiplicity, and survival rate. A significant reduction in tumor incidence (33%), tumor multiplicity (42%), and increase in survival (34%) was observed upon administration of AAILE to NDEA-abused mice. Transmission and scanning electron microscopic investigations showed severe alterations in organelle organization, cellular arrangement, degree of differentiation, cellular metabolism, and morphology of the hepatocytes. These changes appeared to be distinctly delayed upon AAILE supplementation. The results suggest A. indica may have anticancer potential against NDEA-induced hepatic cancer.

Original languageEnglish
Pages (from-to)586-595
Number of pages10
JournalMicroscopy Research and Technique
Volume75
Issue number5
DOIs
Publication statusPublished - 01-05-2012

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Azadirachta
Liver Neoplasms
Tumors
tumors
cancer
Diethylnitrosamine
leaves
mice
incidence
body weight
dosage
organelles
metabolism
Metabolism
Neoplasms
intervals
Scanning
Incidence
scanning
Electrons

All Science Journal Classification (ASJC) codes

  • Anatomy
  • Instrumentation
  • Histology
  • Medical Laboratory Technology

Cite this

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abstract = "The present study was designed to evaluate the anticarcinogenic potential of Azadirachta indica against N-nitrosodiethylamine (NDEA)-induced hepatocarcinogenesis. Further, the associated histopathological and ultrastructural changes were also analyzed. Hepatic cancer model was developed by the intraperitoneal administration of NDEA to mice at weekly intervals, in successive increasing doses, for a period of 8 weeks. Aqueous A. indica leaf extract (AAILE) was administered orally at a dosage of 100 μg/g body weight thrice a week till termination of the study. A relationship between histopathological grading and chemopreventive effect of A. indica had been established at various stages of carcinogenesis. Anticancer activity of A. indica was evaluated in terms of tumor incidence, tumor multiplicity, and survival rate. A significant reduction in tumor incidence (33{\%}), tumor multiplicity (42{\%}), and increase in survival (34{\%}) was observed upon administration of AAILE to NDEA-abused mice. Transmission and scanning electron microscopic investigations showed severe alterations in organelle organization, cellular arrangement, degree of differentiation, cellular metabolism, and morphology of the hepatocytes. These changes appeared to be distinctly delayed upon AAILE supplementation. The results suggest A. indica may have anticancer potential against NDEA-induced hepatic cancer.",
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Azadirachta indica exhibits chemopreventive action against hepatic cancer : Studies on associated histopathological and ultrastructural changes. / Bharati, Sanjay; Rishi, Praveen; Koul, Ashwani.

In: Microscopy Research and Technique, Vol. 75, No. 5, 01.05.2012, p. 586-595.

Research output: Contribution to journalArticle

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