Bioactive PLGA–curcumin microparticle-embedded chitosan scaffold

in vitro and in vivo evaluation

Muthukumar Amirthalingam, Narayanan Kasinathan, Arul Amuthan, Srinivas Mutalik, M. Sreenivasa Reddy, Udupa Nayanabhirama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Wound healing is a complex process affected by several factors. In the present work, novel biocompatible PLGA–curcumin microparticle-embedded chitosan scaffold was fabricated for wound-healing application. Process variables involved in the preparation of microparticles were optimized using design of experiment. Scanning electron microscopy (SEM) confirmed the porous nature of scaffold with embedded microparticles. A maximum release of 14% of the encapsulated curcumin was observed at 12th hour. Modified tube dilution method showed that scaffold significantly (p < 0.05) reduced multiplication of Staphylococcus aureus. More than 50% of the excised wound in rats healed in 4 days with an epithilization period of 18 days.

Original languageEnglish
Pages (from-to)233-241
Number of pages9
JournalArtificial Cells, Nanomedicine and Biotechnology
Volume45
Issue number2
DOIs
Publication statusPublished - 17-02-2017

Fingerprint

Chitosan
Scaffolds
Wound Healing
Curcumin
Electron Scanning Microscopy
Staphylococcus aureus
Design of experiments
Dilution
Rats
Wounds and Injuries
Scanning electron microscopy
In Vitro Techniques

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Pharmaceutical Science

Cite this

@article{a4f6fc20292c48e4a399951c129207f5,
title = "Bioactive PLGA–curcumin microparticle-embedded chitosan scaffold: in vitro and in vivo evaluation",
abstract = "Wound healing is a complex process affected by several factors. In the present work, novel biocompatible PLGA–curcumin microparticle-embedded chitosan scaffold was fabricated for wound-healing application. Process variables involved in the preparation of microparticles were optimized using design of experiment. Scanning electron microscopy (SEM) confirmed the porous nature of scaffold with embedded microparticles. A maximum release of 14{\%} of the encapsulated curcumin was observed at 12th hour. Modified tube dilution method showed that scaffold significantly (p < 0.05) reduced multiplication of Staphylococcus aureus. More than 50{\%} of the excised wound in rats healed in 4 days with an epithilization period of 18 days.",
author = "Muthukumar Amirthalingam and Narayanan Kasinathan and Arul Amuthan and Srinivas Mutalik and {Sreenivasa Reddy}, M. and Udupa Nayanabhirama",
year = "2017",
month = "2",
day = "17",
doi = "10.3109/21691401.2016.1146727",
language = "English",
volume = "45",
pages = "233--241",
journal = "Artificial Cells, Nanomedicine and Biotechnology",
issn = "2169-1401",
publisher = "Informa Healthcare",
number = "2",

}

TY - JOUR

T1 - Bioactive PLGA–curcumin microparticle-embedded chitosan scaffold

T2 - in vitro and in vivo evaluation

AU - Amirthalingam, Muthukumar

AU - Kasinathan, Narayanan

AU - Amuthan, Arul

AU - Mutalik, Srinivas

AU - Sreenivasa Reddy, M.

AU - Nayanabhirama, Udupa

PY - 2017/2/17

Y1 - 2017/2/17

N2 - Wound healing is a complex process affected by several factors. In the present work, novel biocompatible PLGA–curcumin microparticle-embedded chitosan scaffold was fabricated for wound-healing application. Process variables involved in the preparation of microparticles were optimized using design of experiment. Scanning electron microscopy (SEM) confirmed the porous nature of scaffold with embedded microparticles. A maximum release of 14% of the encapsulated curcumin was observed at 12th hour. Modified tube dilution method showed that scaffold significantly (p < 0.05) reduced multiplication of Staphylococcus aureus. More than 50% of the excised wound in rats healed in 4 days with an epithilization period of 18 days.

AB - Wound healing is a complex process affected by several factors. In the present work, novel biocompatible PLGA–curcumin microparticle-embedded chitosan scaffold was fabricated for wound-healing application. Process variables involved in the preparation of microparticles were optimized using design of experiment. Scanning electron microscopy (SEM) confirmed the porous nature of scaffold with embedded microparticles. A maximum release of 14% of the encapsulated curcumin was observed at 12th hour. Modified tube dilution method showed that scaffold significantly (p < 0.05) reduced multiplication of Staphylococcus aureus. More than 50% of the excised wound in rats healed in 4 days with an epithilization period of 18 days.

UR - http://www.scopus.com/inward/record.url?scp=85010434998&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85010434998&partnerID=8YFLogxK

U2 - 10.3109/21691401.2016.1146727

DO - 10.3109/21691401.2016.1146727

M3 - Article

VL - 45

SP - 233

EP - 241

JO - Artificial Cells, Nanomedicine and Biotechnology

JF - Artificial Cells, Nanomedicine and Biotechnology

SN - 2169-1401

IS - 2

ER -