Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

K.R. Prabhakar, V.P. Veeresh, K. Vipan, M. Sudheer, K.I. Priyadarsini, R.B.S.S. Satish, M.K. Unnikrishnan

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Abstract

The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalPhytomedicine
Volume13
Issue number8
DOIs
Publication statusPublished - 2006

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    Prabhakar, K. R., Veeresh, V. P., Vipan, K., Sudheer, M., Priyadarsini, K. I., Satish, R. B. S. S., & Unnikrishnan, M. K. (2006). Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. Phytomedicine, 13(8), 591-595. https://doi.org/10.1016/j.phymed.2005.07.003