Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

K.R. Prabhakar, V.P. Veeresh, K. Vipan, M. Sudheer, K.I. Priyadarsini, R.B.S.S. Satish, M.K. Unnikrishnan

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Abstract

The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.
Original languageEnglish
Pages (from-to)591-595
Number of pages5
JournalPhytomedicine
Volume13
Issue number8
DOIs
Publication statusPublished - 2006

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Hydroxyl Radical
Free Radicals
Pulse Radiolysis
Plant Extracts
Flavonoids
DNA Damage
Nitric Oxide
2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid
Fenton's reagent
potassium thiocyanate
thiocyanate
calf thymus DNA

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Prabhakar, K. R., Veeresh, V. P., Vipan, K., Sudheer, M., Priyadarsini, K. I., Satish, R. B. S. S., & Unnikrishnan, M. K. (2006). Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. Phytomedicine, 13(8), 591-595. https://doi.org/10.1016/j.phymed.2005.07.003
Prabhakar, K.R. ; Veeresh, V.P. ; Vipan, K. ; Sudheer, M. ; Priyadarsini, K.I. ; Satish, R.B.S.S. ; Unnikrishnan, M.K. / Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. In: Phytomedicine. 2006 ; Vol. 13, No. 8. pp. 591-595.
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abstract = "The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13{\%} inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31{\%} scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. {\circledC} 2005 Elsevier GmbH. All rights reserved.",
author = "K.R. Prabhakar and V.P. Veeresh and K. Vipan and M. Sudheer and K.I. Priyadarsini and R.B.S.S. Satish and M.K. Unnikrishnan",
note = "Cited By :27 Export Date: 10 November 2017 CODEN: PYTOE Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: mkunnikrishnan@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; hydroxyl radical, 3352-57-6; nitric oxide, 10102-43-9; thiocyanate potassium, 333-20-0; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Benzothiazoles; Free Radical Scavengers; Hydroxyl Radical, 3352-57-6; Nitric Oxide, 10102-43-9; Picrates; Plant Extracts; Sulfonic Acids References: Adelman, R., Saul, R.L., Ames, B., Oxidative damage to DNA: relation to species metabolic rate and life-span (1988) Proc. Natl. Acad.Sci. USA, 85, pp. 2706-2708; Clardy, J., Walsh, C., Lessons from natural molecules (review) (2004) Nature, 432, pp. 829-837; De Ruiz, R.E.L., Fusco, M., Sosa A and Ruiz, S.O., Constituents of Coronopus didymus (1994) Fitoterpia, 65, pp. 181-182; Inam ul, H., Composition of essential oil of Coronopus didymus seeds (1989) J. Chem. Soc. Pakistan, 11, pp. 80-81; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int. J. Pharmaceut., 57, pp. 173-176; Lu, C., Koropatnick, J., George, C.M., Roles of vitamin C in radiation-induced DNA damage in presence and absence of copper (2001) Chemico-Biol. Interact., 137, pp. 75-88; McDowall, F.H., Mortan, I.D., McDowell, A.K.R., Land-cress taint in cream and butter (1947) NZ J. Sci. Technol. A, 28, pp. 305-307; Mishra, B., Priyadarsini, K.I., Sudheer Kumar, M., Unnikrishnan, M.K., Effect of O-glycosilation on the antioxidant activity and free radical reactions of a plant flavonoid, Chrysoeriol (2003) Bioorg. Med. Chem., 11 (13), pp. 2677-2685; Prabhakar, K.R., Srinivasan, K.K., Padma, G.M.R., Chemical investigation, anti-inflammatory and wound healing properties of Coronopus didymus (2002) Pharm. Biol., 40, pp. 490-493; Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Pryor, W.A., Porter, N.A., Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids (1990) Free Radic. Biol. Med., 8, pp. 541-543; Raj Sekhar Narla, Rao, M.N.A., Scavenging of free radicals and inhibition of lipid peroxidation by 3-phenyl syndone (1995) J. Pharm. Pharmacol., 47, pp. 623-625; Spinks, J.W.T., Woods, R.J., (1990) An Introduction to Radiation Chemistry, , Wiley, New York; Sudheer Kumar, M., Jagadish, S.R., Kiran Babu, S., Unnikrishnan, M.K., In-vitro evaluation of antioxidant properties of Cocus nucifera Linn Water (2003) Nahrung/Food, 47 (2), pp. 126-131; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by the Ocimum flavonoids Orientin and Vicenin: mechanism of action (2000) Radiat. Res., 154, pp. 455-460; Weiss, J.F., Landauer, M.R., Protection against ionizing radiation by antioxidant nutrients and Phytochemicals (2003) Toxicology, 189, pp. 1-20",
year = "2006",
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pages = "591--595",
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}

Prabhakar, KR, Veeresh, VP, Vipan, K, Sudheer, M, Priyadarsini, KI, Satish, RBSS & Unnikrishnan, MK 2006, 'Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective', Phytomedicine, vol. 13, no. 8, pp. 591-595. https://doi.org/10.1016/j.phymed.2005.07.003

Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective. / Prabhakar, K.R.; Veeresh, V.P.; Vipan, K.; Sudheer, M.; Priyadarsini, K.I.; Satish, R.B.S.S.; Unnikrishnan, M.K.

In: Phytomedicine, Vol. 13, No. 8, 2006, p. 591-595.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Bioactivity-guided fractionation of Coronopus didymus: A free radical scavenging perspective

AU - Prabhakar, K.R.

AU - Veeresh, V.P.

AU - Vipan, K.

AU - Sudheer, M.

AU - Priyadarsini, K.I.

AU - Satish, R.B.S.S.

AU - Unnikrishnan, M.K.

N1 - Cited By :27 Export Date: 10 November 2017 CODEN: PYTOE Correspondence Address: Unnikrishnan, M.K.; Department of Pharmacology, Manipal College of Pharmaceutical Sciences, Manipal, 576104, India; email: mkunnikrishnan@gmail.com Chemicals/CAS: 1,1 diphenyl 2 picrylhydrazyl, 1898-66-4; hydroxyl radical, 3352-57-6; nitric oxide, 10102-43-9; thiocyanate potassium, 333-20-0; 2,2'-azino-di-(3-ethylbenzothiazoline)-6-sulfonic acid, 28752-68-3; 2,2-diphenyl-1-picrylhydrazyl, 1898-66-4; Benzothiazoles; Free Radical Scavengers; Hydroxyl Radical, 3352-57-6; Nitric Oxide, 10102-43-9; Picrates; Plant Extracts; Sulfonic Acids References: Adelman, R., Saul, R.L., Ames, B., Oxidative damage to DNA: relation to species metabolic rate and life-span (1988) Proc. Natl. Acad.Sci. USA, 85, pp. 2706-2708; Clardy, J., Walsh, C., Lessons from natural molecules (review) (2004) Nature, 432, pp. 829-837; De Ruiz, R.E.L., Fusco, M., Sosa A and Ruiz, S.O., Constituents of Coronopus didymus (1994) Fitoterpia, 65, pp. 181-182; Inam ul, H., Composition of essential oil of Coronopus didymus seeds (1989) J. Chem. Soc. Pakistan, 11, pp. 80-81; Kunchandy, E., Rao, M.N.A., Effect of curcumin on hydroxyl radical generation through Fenton reaction (1989) Int. J. Pharmaceut., 57, pp. 173-176; Lu, C., Koropatnick, J., George, C.M., Roles of vitamin C in radiation-induced DNA damage in presence and absence of copper (2001) Chemico-Biol. Interact., 137, pp. 75-88; McDowall, F.H., Mortan, I.D., McDowell, A.K.R., Land-cress taint in cream and butter (1947) NZ J. Sci. Technol. A, 28, pp. 305-307; Mishra, B., Priyadarsini, K.I., Sudheer Kumar, M., Unnikrishnan, M.K., Effect of O-glycosilation on the antioxidant activity and free radical reactions of a plant flavonoid, Chrysoeriol (2003) Bioorg. Med. Chem., 11 (13), pp. 2677-2685; Prabhakar, K.R., Srinivasan, K.K., Padma, G.M.R., Chemical investigation, anti-inflammatory and wound healing properties of Coronopus didymus (2002) Pharm. Biol., 40, pp. 490-493; Prieto, P., Pineda, M., Aguilar, M., Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E (1999) Anal. Biochem., 269, pp. 337-341; Pryor, W.A., Porter, N.A., Suggested mechanisms for the production of 4-hydroxy-2-nonenal from the autoxidation of polyunsaturated fatty acids (1990) Free Radic. Biol. Med., 8, pp. 541-543; Raj Sekhar Narla, Rao, M.N.A., Scavenging of free radicals and inhibition of lipid peroxidation by 3-phenyl syndone (1995) J. Pharm. Pharmacol., 47, pp. 623-625; Spinks, J.W.T., Woods, R.J., (1990) An Introduction to Radiation Chemistry, , Wiley, New York; Sudheer Kumar, M., Jagadish, S.R., Kiran Babu, S., Unnikrishnan, M.K., In-vitro evaluation of antioxidant properties of Cocus nucifera Linn Water (2003) Nahrung/Food, 47 (2), pp. 126-131; Uma Devi, P., Ganasoundari, A., Vrinda, B., Srinivasan, K.K., Unnikrishnan, M.K., Radiation protection by the Ocimum flavonoids Orientin and Vicenin: mechanism of action (2000) Radiat. Res., 154, pp. 455-460; Weiss, J.F., Landauer, M.R., Protection against ionizing radiation by antioxidant nutrients and Phytochemicals (2003) Toxicology, 189, pp. 1-20

PY - 2006

Y1 - 2006

N2 - The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.

AB - The whole plant aqueous extract of Coronopus didymus Linn. was fractionated on the basis of polarity and resulting fractions were evaluated for free radical scavenging ability. The most non-polar fraction (CDF1) was found to be more active than other fractions in scavenging DPPH, ABTS{radical dot}-, nitric oxide and hydroxyl radicals in steady-state conditions. Stop-flow spectrometric studies showed 58.13% inhibition of 100 μM DPPH at a concentration of 150 μg/ml of CDF1 in 1000 s and 32.31% scavenging of 960 μM ABTS{radical dot}- at a concentration of 300 μg/ml of CDF1 in 100 s. The reaction of CDF1 with hydroxyl radicals produced by pulse radiolysis showed a transient spectrum with absorption peaks at 320, 390 and 400 nm, indicating the presence of flavonoids/related components. Competition kinetics with potassium thiocyanate against scavenging of hydroxyl radicals showed a reactivity of 0.1326 against thiocyanate. CDF1 also protected against Fenton reagent-induced calf thymus DNA damage at a concentration of 400 mg/ml indicating it to be the most potent fraction. © 2005 Elsevier GmbH. All rights reserved.

U2 - 10.1016/j.phymed.2005.07.003

DO - 10.1016/j.phymed.2005.07.003

M3 - Article

VL - 13

SP - 591

EP - 595

JO - Phytomedicine

JF - Phytomedicine

SN - 0944-7113

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