TY - JOUR
T1 - Bioanalytical RP-HPLC method validation for resveratrol and its application to pharmacokinetic and drug distribution studies
AU - Gadag, Shivaprasad
AU - Narayan, Reema
AU - Nayak, Yogendra
AU - Nayak, Usha Yogendra
N1 - Funding Information:
This work was supported by the Science and Engineering Research Board, Dept. of Science and Technology, New Delhi, India (Ref. No. EMR/2016/007006).
Funding Information:
This work was supported by the Science and Engineering Research Board, Dept. of Science and Technology, New Delhi,
Publisher Copyright:
© 2022. Shivaprasad Gadag et al. This is an open access article distributed under the terms of the Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/).
PY - 2022/2
Y1 - 2022/2
N2 - In the present study, an attempt was made to develop and validate a highly sensitive reverse phase-High Pressure Liquid Chromatography (HPLC) method for the quantification of resveratrol (RVT) in the plasma and tissue matrix. Pharmacokinetics and tissue distribution studies were carried out in rats after oral administration of RVT. RVT was eluted using 0.1% orthophosphoric acid and acetonitrile as the mobile phase. The developed method showed good recovery and linearity without any interference from the biological matrix. From the pharmacokinetic studies, the plasma half-life of RVT was found to be 4.4 ± 0.12 hours. It was observed from bio-distribution studies that RVT preferentially accumulated in the high perfused organs. The developed method was successfully applied for the quantification of RVT in different biological matrices
AB - In the present study, an attempt was made to develop and validate a highly sensitive reverse phase-High Pressure Liquid Chromatography (HPLC) method for the quantification of resveratrol (RVT) in the plasma and tissue matrix. Pharmacokinetics and tissue distribution studies were carried out in rats after oral administration of RVT. RVT was eluted using 0.1% orthophosphoric acid and acetonitrile as the mobile phase. The developed method showed good recovery and linearity without any interference from the biological matrix. From the pharmacokinetic studies, the plasma half-life of RVT was found to be 4.4 ± 0.12 hours. It was observed from bio-distribution studies that RVT preferentially accumulated in the high perfused organs. The developed method was successfully applied for the quantification of RVT in different biological matrices
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U2 - 10.7324/JAPS.2021.120216
DO - 10.7324/JAPS.2021.120216
M3 - Article
AN - SCOPUS:85124566928
VL - 12
SP - 158
EP - 164
JO - Journal of Applied Pharmaceutical Science
JF - Journal of Applied Pharmaceutical Science
SN - 2231-3354
IS - 2
ER -