Bioavailability and pharmacokinetics of vasicine in wistar rats

H.N. Aswatha Ram, A. Shirwaikar

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

To determine vasicine in rat blood plasma by HPLC and to study its bioavailability and pharmacokinetics. Vasicine (>97% pure by HPLC) was purchased from SPIC Pharmaceuticals Division, Chennai, India. The standard plot was prepared by spiking the various concentrations of pure vasicine into the normal rat blood plasma followed by extraction and HPLC determination. A single oral dose of 0.065mg/Kg of pure vasicine was administered to all the rats. Plasma levels of vasicine were analysed by using HPLC. Vasicine was extracted from the rat blood plasma by adding 10N hydrochloric acid at pH 6.2 for the precipitation of plasma proteins followed by extraction into chloroform and the chloroform extract was evaporated to dryness to obtain the vasicine residue. The residue was reconstituted in methanol (1ml) and then injected into the HPLC system. The standard plot was linear for various concentrations studied with linear regression coefficient R 2 of 0.9903. Accuracy was established by the percent recovery at all the concentrations of vasicine and the mean recovery was found to be 108.73%±7.845. The mean peak plasma vasicine concentration of 12.8 ng/ml was observed at 4 h. The present study shows determination of pure vasicine in rat plasma by HPLC. The various pharmacokinetic parameters for vasicine were studied.
Original languageEnglish
Pages (from-to)53-60
Number of pages8
JournalPharmacologyonline
Volume2
Publication statusPublished - 2007

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Biological Availability
Wistar Rats
Pharmacokinetics
High Pressure Liquid Chromatography
Chloroform
vasicine
Hydrochloric Acid
Methanol
Blood Proteins
India
Linear Models

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title = "Bioavailability and pharmacokinetics of vasicine in wistar rats",
abstract = "To determine vasicine in rat blood plasma by HPLC and to study its bioavailability and pharmacokinetics. Vasicine (>97{\%} pure by HPLC) was purchased from SPIC Pharmaceuticals Division, Chennai, India. The standard plot was prepared by spiking the various concentrations of pure vasicine into the normal rat blood plasma followed by extraction and HPLC determination. A single oral dose of 0.065mg/Kg of pure vasicine was administered to all the rats. Plasma levels of vasicine were analysed by using HPLC. Vasicine was extracted from the rat blood plasma by adding 10N hydrochloric acid at pH 6.2 for the precipitation of plasma proteins followed by extraction into chloroform and the chloroform extract was evaporated to dryness to obtain the vasicine residue. The residue was reconstituted in methanol (1ml) and then injected into the HPLC system. The standard plot was linear for various concentrations studied with linear regression coefficient R 2 of 0.9903. Accuracy was established by the percent recovery at all the concentrations of vasicine and the mean recovery was found to be 108.73{\%}±7.845. The mean peak plasma vasicine concentration of 12.8 ng/ml was observed at 4 h. The present study shows determination of pure vasicine in rat plasma by HPLC. The various pharmacokinetic parameters for vasicine were studied.",
author = "{Aswatha Ram}, H.N. and A. Shirwaikar",
note = "Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal-576 104 Karnataka, India; email: aswatharam@yahoo.com Chemicals/CAS: chloroform, 67-66-3; hydrochloric acid, 7647-01-0; methanol, 67-56-1; vasicine, 6159-55-3 References: The Wealth of India, (Publication and Information Directorate, CSIR, New Delhi) 1948, I; Nadakarni, K.M., (1976) Indian Materia Medica, 1. , Popular Prakashan, Bombay; Atal, C.K., (1980) Chemistry and Pharmacology of Vasicine, (Director, RRL, Jammu Tawi), p. 41; Parikh, K.M., Doshi, V.J., Salunkhe, J.B., Kamath, R.P., High Performance Liquid Chromatographic determination of Vasicine and Vasicine from pharmaceutical formulations (1989) Indian Drugs, 27, pp. 64-66; Francis, M., Sane, R.T., Tipins, S., Simultaneous HPLC method for determination of vasicine and glycyrrhizin from herbal preparations (2003) Indian Drugs, 40, pp. 712-715; Gaitonde, B.V., Ulhas, V., Bhat, (1988) TLC-Spectrophotometric determination of vasicine alkaloid from marketed formulation of Adhatoda vasica, Nees, Adli Tip Ders, 4, pp. 15-17; Amla V, Bano G, Johri RK, Zutshi U, Atal CK. Pharmacokinetics of vasicine in healthy Indian volunteers, Zhongguo Yaoli Xuebao, 1987;B(2):190-2; Indian Herbal Pharmacopoeia, (IDMA and RRL (CSIR), Jammu Tawi) 1998; Paget, G.E., Barnes, J.M., (1964) Evaluation of Drug Activities: Pharmacometrics, , edited by Laurence DR, Bacharach AL Academic Press, New York; PK Solutions 2.0 (Version-Windows 2.0.6), Noncompartmental Pharmacokinetics Data Analysis, Pharmacokinetics and Metabolism Software, Summit Research Services, Open Field, CO, USA, 1999UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548531957&partnerID=40&md5=0b93a13678d969f23145fc5f727429d7",
year = "2007",
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journal = "Pharmacologyonline",
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publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

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Bioavailability and pharmacokinetics of vasicine in wistar rats. / Aswatha Ram, H.N.; Shirwaikar, A.

In: Pharmacologyonline, Vol. 2, 2007, p. 53-60.

Research output: Contribution to journalArticle

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N1 - Export Date: 10 November 2017 Correspondence Address: Aswatha Ram, H.N.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal-576 104 Karnataka, India; email: aswatharam@yahoo.com Chemicals/CAS: chloroform, 67-66-3; hydrochloric acid, 7647-01-0; methanol, 67-56-1; vasicine, 6159-55-3 References: The Wealth of India, (Publication and Information Directorate, CSIR, New Delhi) 1948, I; Nadakarni, K.M., (1976) Indian Materia Medica, 1. , Popular Prakashan, Bombay; Atal, C.K., (1980) Chemistry and Pharmacology of Vasicine, (Director, RRL, Jammu Tawi), p. 41; Parikh, K.M., Doshi, V.J., Salunkhe, J.B., Kamath, R.P., High Performance Liquid Chromatographic determination of Vasicine and Vasicine from pharmaceutical formulations (1989) Indian Drugs, 27, pp. 64-66; Francis, M., Sane, R.T., Tipins, S., Simultaneous HPLC method for determination of vasicine and glycyrrhizin from herbal preparations (2003) Indian Drugs, 40, pp. 712-715; Gaitonde, B.V., Ulhas, V., Bhat, (1988) TLC-Spectrophotometric determination of vasicine alkaloid from marketed formulation of Adhatoda vasica, Nees, Adli Tip Ders, 4, pp. 15-17; Amla V, Bano G, Johri RK, Zutshi U, Atal CK. Pharmacokinetics of vasicine in healthy Indian volunteers, Zhongguo Yaoli Xuebao, 1987;B(2):190-2; Indian Herbal Pharmacopoeia, (IDMA and RRL (CSIR), Jammu Tawi) 1998; Paget, G.E., Barnes, J.M., (1964) Evaluation of Drug Activities: Pharmacometrics, , edited by Laurence DR, Bacharach AL Academic Press, New York; PK Solutions 2.0 (Version-Windows 2.0.6), Noncompartmental Pharmacokinetics Data Analysis, Pharmacokinetics and Metabolism Software, Summit Research Services, Open Field, CO, USA, 1999UR - https://www.scopus.com/inward/record.uri?eid=2-s2.0-34548531957&partnerID=40&md5=0b93a13678d969f23145fc5f727429d7

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N2 - To determine vasicine in rat blood plasma by HPLC and to study its bioavailability and pharmacokinetics. Vasicine (>97% pure by HPLC) was purchased from SPIC Pharmaceuticals Division, Chennai, India. The standard plot was prepared by spiking the various concentrations of pure vasicine into the normal rat blood plasma followed by extraction and HPLC determination. A single oral dose of 0.065mg/Kg of pure vasicine was administered to all the rats. Plasma levels of vasicine were analysed by using HPLC. Vasicine was extracted from the rat blood plasma by adding 10N hydrochloric acid at pH 6.2 for the precipitation of plasma proteins followed by extraction into chloroform and the chloroform extract was evaporated to dryness to obtain the vasicine residue. The residue was reconstituted in methanol (1ml) and then injected into the HPLC system. The standard plot was linear for various concentrations studied with linear regression coefficient R 2 of 0.9903. Accuracy was established by the percent recovery at all the concentrations of vasicine and the mean recovery was found to be 108.73%±7.845. The mean peak plasma vasicine concentration of 12.8 ng/ml was observed at 4 h. The present study shows determination of pure vasicine in rat plasma by HPLC. The various pharmacokinetic parameters for vasicine were studied.

AB - To determine vasicine in rat blood plasma by HPLC and to study its bioavailability and pharmacokinetics. Vasicine (>97% pure by HPLC) was purchased from SPIC Pharmaceuticals Division, Chennai, India. The standard plot was prepared by spiking the various concentrations of pure vasicine into the normal rat blood plasma followed by extraction and HPLC determination. A single oral dose of 0.065mg/Kg of pure vasicine was administered to all the rats. Plasma levels of vasicine were analysed by using HPLC. Vasicine was extracted from the rat blood plasma by adding 10N hydrochloric acid at pH 6.2 for the precipitation of plasma proteins followed by extraction into chloroform and the chloroform extract was evaporated to dryness to obtain the vasicine residue. The residue was reconstituted in methanol (1ml) and then injected into the HPLC system. The standard plot was linear for various concentrations studied with linear regression coefficient R 2 of 0.9903. Accuracy was established by the percent recovery at all the concentrations of vasicine and the mean recovery was found to be 108.73%±7.845. The mean peak plasma vasicine concentration of 12.8 ng/ml was observed at 4 h. The present study shows determination of pure vasicine in rat plasma by HPLC. The various pharmacokinetic parameters for vasicine were studied.

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