Bioavailability and pharmacokinetics of vasicine in wistar rats

To determine vasicine in rat blood plasma by HPLC and to study its bioavailability and pharmacokinetics. Vasicine (>97% pure by HPLC) was purchased from SPIC Pharmaceuticals Division, Chennai, India. The standard plot was prepared by spiking the various concentrations of pure vasicine into the normal rat blood plasma followed by extraction and HPLC determination. A single oral dose of 0.065mg/Kg of pure vasicine was administered to all the rats. Plasma levels of vasicine were analysed by using HPLC. Vasicine was extracted from the rat blood plasma by adding 10N hydrochloric acid at pH 6.2 for the precipitation of plasma proteins followed by extraction into chloroform and the chloroform extract was evaporated to dryness to obtain the vasicine residue. The residue was reconstituted in methanol (1ml) and then injected into the HPLC system. The standard plot was linear for various concentrations studied with linear regression coefficient R 2 of 0.9903. Accuracy was established by the percent recovery at all the concentrations of vasicine and the mean recovery was found to be 108.73%±7.845. The mean peak plasma vasicine concentration of 12.8 ng/ml was observed at 4 h. The present study shows determination of pure vasicine in rat plasma by HPLC. The various pharmacokinetic parameters for vasicine were studied.