Biochemical analysis of granulation tissue in steroid and Centella asiatica (Linn) treated rats

B. Somashekar Shetty, S. L. Udupa, A. L. Udupa

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The present study was undertaken to investigate the effect of alcoholic extract of Centella asiatica Linn in normal and steroid suppressed healing in dead space wound model in rats. Albino rats of either sex were divided into 4 groups. Group 1: Control group with wound alone; Group II: Test group with wound and treated with extract; Group III: Test group with wound and treated with steroid; Group IV: Test group with wound treated with steroid and extract. Granulation tissue was created by making dead space wound and used for analyzing wound healing relevant biochemical parameters such as hydroxyproline, glycosaminoglycan content, tissue protein, lysyl oxidase and antioxidant profiles. Significant increase in hydroxyproline and glycosaminoglycans content in granulation tissue were observed. The significant elevated levels of antioxidants, protein and lysyl oxidase concentrations were observed in extract treated rats. Present results indicated that the leaf extract promotes wound healing significantly and able to overcome the wound healing suppressing action of steroid.

Original languageEnglish
Pages (from-to)624-632
Number of pages9
Publication statusPublished - 13-10-2008


All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Drug Discovery

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