Abstract
Though chitosan hydrogels have shown potential for numerous tissue regeneration applications, its insolubility above pH 6.0 have greatly limited applicability in encapsulation of live cells and pH sensitive bioactive agents. In this report an approach to overcome this limitation was tested with phosphorylated derivative of chitosan, N-methylene phosphonic chitosan (NMPC). NMPC is also known to possess efficacious gene delivery, biomineralization, and osteoinduction properties. NMPC hydrogels were synthesized by cross-linking NMPC with either glutaraldehyde (NMPC-GLU) or genipin (NMPC-GEN). An increase in pH from 3.0 to 6.8 reduced gelation time with both the agents, with the reduction more pronounced in NMPC-GLU. The results correlated with the reaction mechanisms of both agents with NMPC. Further NMPC-GEN gels demonstrated higher elastic modulus and compressive strength values. Cell culture studies showed higher cell proliferation on NMPC-GEN compared to NMPC-GLU.
| Original language | English |
|---|---|
| Pages (from-to) | 27-35 |
| Number of pages | 9 |
| Journal | Soft Materials |
| Volume | 12 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - 01-01-2014 |
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All Science Journal Classification (ASJC) codes
- Chemistry(all)
- Materials Science(all)
- Condensed Matter Physics
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Biofunctional phosphorylated chitosan hydrogels prepared above pH 6 and effect of crosslinkers on gel properties towards biomedical applications. / Datta, Pallab; Thakur, Goutam; Chatterjee, Jyotirmoy; Dhara, Santanu.
In: Soft Materials, Vol. 12, No. 1, 01.01.2014, p. 27-35.Research output: Contribution to journal › Article
TY - JOUR
T1 - Biofunctional phosphorylated chitosan hydrogels prepared above pH 6 and effect of crosslinkers on gel properties towards biomedical applications
AU - Datta, Pallab
AU - Thakur, Goutam
AU - Chatterjee, Jyotirmoy
AU - Dhara, Santanu
PY - 2014/1/1
Y1 - 2014/1/1
N2 - Though chitosan hydrogels have shown potential for numerous tissue regeneration applications, its insolubility above pH 6.0 have greatly limited applicability in encapsulation of live cells and pH sensitive bioactive agents. In this report an approach to overcome this limitation was tested with phosphorylated derivative of chitosan, N-methylene phosphonic chitosan (NMPC). NMPC is also known to possess efficacious gene delivery, biomineralization, and osteoinduction properties. NMPC hydrogels were synthesized by cross-linking NMPC with either glutaraldehyde (NMPC-GLU) or genipin (NMPC-GEN). An increase in pH from 3.0 to 6.8 reduced gelation time with both the agents, with the reduction more pronounced in NMPC-GLU. The results correlated with the reaction mechanisms of both agents with NMPC. Further NMPC-GEN gels demonstrated higher elastic modulus and compressive strength values. Cell culture studies showed higher cell proliferation on NMPC-GEN compared to NMPC-GLU.
AB - Though chitosan hydrogels have shown potential for numerous tissue regeneration applications, its insolubility above pH 6.0 have greatly limited applicability in encapsulation of live cells and pH sensitive bioactive agents. In this report an approach to overcome this limitation was tested with phosphorylated derivative of chitosan, N-methylene phosphonic chitosan (NMPC). NMPC is also known to possess efficacious gene delivery, biomineralization, and osteoinduction properties. NMPC hydrogels were synthesized by cross-linking NMPC with either glutaraldehyde (NMPC-GLU) or genipin (NMPC-GEN). An increase in pH from 3.0 to 6.8 reduced gelation time with both the agents, with the reduction more pronounced in NMPC-GLU. The results correlated with the reaction mechanisms of both agents with NMPC. Further NMPC-GEN gels demonstrated higher elastic modulus and compressive strength values. Cell culture studies showed higher cell proliferation on NMPC-GEN compared to NMPC-GLU.
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UR - http://www.scopus.com/inward/citedby.url?scp=84881328288&partnerID=8YFLogxK
U2 - 10.1080/1539445X.2012.735315
DO - 10.1080/1539445X.2012.735315
M3 - Article
VL - 12
SP - 27
EP - 35
JO - Soft Materials
JF - Soft Materials
SN - 1539-445X
IS - 1
ER -