Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates

M. S. Divyashree, J. George, R. Agrawal

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Readily available and low cost terpenic compounds like (+) limonene, (-) limonene, (+) α-pinene, (-) α-pinene and (-) β-pinene, were used as substrates for biotransformation by a local fungal isolate of Aspergillus niger and a bacterial strain of Pseudomonas putida. The cultures were grown in potato dextrose broth at 30°C to their exponential phase and harvested. The resting cells were taken in phosphate buffer (0.05M; pH 7.0). Each substrate was added separately (2 mg/25 ml buffer) and incubated at 30°C for 6 h, after the reaction the flavouring compounds were extracted in dichloromethane, dried over anhydrous sodium sulphate, identified and analysed by GCMS. Aspergillus niger could use (-) limonene as the substrate to give carveol (18%) and dihydrocarveol (15%). Bioconversion from (+) α-pinene was better with Pseudomonas putida, producing industrially important compounds like verbenol (35%), dihydrocarveol acetate (20%) and verbenone (10%) in high yields. However, the bacteria was unable to bioconvert (-) α-pinene, (-) β-pinene, and (-) (+) limonenes to these important compounds.

Original languageEnglish
Pages (from-to)73-76
Number of pages4
JournalJournal of Food Science and Technology
Volume43
Issue number1
Publication statusPublished - 01-01-2006

Fingerprint

Pseudomonas putida
Aspergillus niger
Biotransformation
biotransformation
Buffers
buffers
verbenone
sodium sulfate
Methylene Chloride
flavorings
methylene chloride
cells
Solanum tuberosum
terpenoids
Gas Chromatography-Mass Spectrometry
Acetates
Phosphates
acetates
potatoes
phosphates

All Science Journal Classification (ASJC) codes

  • Food Science

Cite this

@article{19b8b9dfb86f491389964796a3755ea8,
title = "Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates",
abstract = "Readily available and low cost terpenic compounds like (+) limonene, (-) limonene, (+) α-pinene, (-) α-pinene and (-) β-pinene, were used as substrates for biotransformation by a local fungal isolate of Aspergillus niger and a bacterial strain of Pseudomonas putida. The cultures were grown in potato dextrose broth at 30°C to their exponential phase and harvested. The resting cells were taken in phosphate buffer (0.05M; pH 7.0). Each substrate was added separately (2 mg/25 ml buffer) and incubated at 30°C for 6 h, after the reaction the flavouring compounds were extracted in dichloromethane, dried over anhydrous sodium sulphate, identified and analysed by GCMS. Aspergillus niger could use (-) limonene as the substrate to give carveol (18{\%}) and dihydrocarveol (15{\%}). Bioconversion from (+) α-pinene was better with Pseudomonas putida, producing industrially important compounds like verbenol (35{\%}), dihydrocarveol acetate (20{\%}) and verbenone (10{\%}) in high yields. However, the bacteria was unable to bioconvert (-) α-pinene, (-) β-pinene, and (-) (+) limonenes to these important compounds.",
author = "Divyashree, {M. S.} and J. George and R. Agrawal",
year = "2006",
month = "1",
day = "1",
language = "English",
volume = "43",
pages = "73--76",
journal = "Journal of Food Science and Technology",
issn = "0022-1155",
publisher = "Springer India",
number = "1",

}

Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates. / Divyashree, M. S.; George, J.; Agrawal, R.

In: Journal of Food Science and Technology, Vol. 43, No. 1, 01.01.2006, p. 73-76.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Biotransformation of terpenic substrates by resting cells of Aspergillus niger and Pseudomonas putida isolates

AU - Divyashree, M. S.

AU - George, J.

AU - Agrawal, R.

PY - 2006/1/1

Y1 - 2006/1/1

N2 - Readily available and low cost terpenic compounds like (+) limonene, (-) limonene, (+) α-pinene, (-) α-pinene and (-) β-pinene, were used as substrates for biotransformation by a local fungal isolate of Aspergillus niger and a bacterial strain of Pseudomonas putida. The cultures were grown in potato dextrose broth at 30°C to their exponential phase and harvested. The resting cells were taken in phosphate buffer (0.05M; pH 7.0). Each substrate was added separately (2 mg/25 ml buffer) and incubated at 30°C for 6 h, after the reaction the flavouring compounds were extracted in dichloromethane, dried over anhydrous sodium sulphate, identified and analysed by GCMS. Aspergillus niger could use (-) limonene as the substrate to give carveol (18%) and dihydrocarveol (15%). Bioconversion from (+) α-pinene was better with Pseudomonas putida, producing industrially important compounds like verbenol (35%), dihydrocarveol acetate (20%) and verbenone (10%) in high yields. However, the bacteria was unable to bioconvert (-) α-pinene, (-) β-pinene, and (-) (+) limonenes to these important compounds.

AB - Readily available and low cost terpenic compounds like (+) limonene, (-) limonene, (+) α-pinene, (-) α-pinene and (-) β-pinene, were used as substrates for biotransformation by a local fungal isolate of Aspergillus niger and a bacterial strain of Pseudomonas putida. The cultures were grown in potato dextrose broth at 30°C to their exponential phase and harvested. The resting cells were taken in phosphate buffer (0.05M; pH 7.0). Each substrate was added separately (2 mg/25 ml buffer) and incubated at 30°C for 6 h, after the reaction the flavouring compounds were extracted in dichloromethane, dried over anhydrous sodium sulphate, identified and analysed by GCMS. Aspergillus niger could use (-) limonene as the substrate to give carveol (18%) and dihydrocarveol (15%). Bioconversion from (+) α-pinene was better with Pseudomonas putida, producing industrially important compounds like verbenol (35%), dihydrocarveol acetate (20%) and verbenone (10%) in high yields. However, the bacteria was unable to bioconvert (-) α-pinene, (-) β-pinene, and (-) (+) limonenes to these important compounds.

UR - http://www.scopus.com/inward/record.url?scp=29244445773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=29244445773&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:29244445773

VL - 43

SP - 73

EP - 76

JO - Journal of Food Science and Technology

JF - Journal of Food Science and Technology

SN - 0022-1155

IS - 1

ER -