Bone marrow biopsy in non-Hodgkin lymphoma: A morphological study

Suneet Kumar, Aarathi R. Rau, Ramadas Naik, Hema Kini, Alka M. Mathai, Muktha R. Pai, Urmila N. Khadilkar

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Context: Bone marrow (BM) biopsy is an integral part of staging work-up for non-Hodgkin lymphoma (NHL). Aims: To study the characteristics of BM involvement in NHL with respect to incidence, histologic pattern and morphology of infiltration and its discordance with the histology of primary anatomic site. Settings and Design: Forty-nine cases of NHL in which BM biopsy was performed for staging were included in this study, the primary site being classified according to the WHO classification for NHL. Materials and Methods: A prospective study of 49 cases was conducted. Bilateral BM biopsy was obtained from the posterior superior iliac spine. The biopsies were fixed in 10% buffered formalin solution and decalcified using 10% formal - formic acid for 4 - 6 h followed by routine processing. The serial sections were stained by hematoxylin and eosin and reticulin stains. Results: BM biopsy showed involvement by lymphoma in 27 cases (55.10%). Unilateral positivity was found in four cases (14.81% cases). The overall incidence of marrow involvement by NHL was 55.1%. The incidence of involvement was higher in T-cell lymphomas when compared with B-cell lymphomas and predominant pattern of involvement was mixed. Diffuse large B-cell lymphomas had the lowest incidence in all the B-cell lymphomas. A discordant histology between BM and primary anatomic site was found in 29.63% (8/27) of the cases, where it was seen more in follicular lymphomas and diffuse large B-cell lymphomas. Conclusions: Critical examination of BM biopsies can increase the diagnostic accuracy, thereby contributing to the prognosis and appropriate treatment modalities.

Original languageEnglish
Pages (from-to)332-338
Number of pages7
JournalIndian Journal of Pathology and Microbiology
Volume52
Issue number3
DOIs
Publication statusPublished - 01-07-2009

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Non-Hodgkin's Lymphoma
Bone Marrow
Biopsy
formic acid
Lymphoma, Large B-Cell, Diffuse
Incidence
B-Cell Lymphoma
Histology
Reticulin
Bone Marrow Examination
Follicular Lymphoma
T-Cell Lymphoma
Hematoxylin
Eosine Yellowish-(YS)
Formaldehyde
Lymphoma
Spine
Coloring Agents
Prospective Studies

All Science Journal Classification (ASJC) codes

  • Pathology and Forensic Medicine
  • Microbiology (medical)

Cite this

Kumar, Suneet ; Rau, Aarathi R. ; Naik, Ramadas ; Kini, Hema ; Mathai, Alka M. ; Pai, Muktha R. ; Khadilkar, Urmila N. / Bone marrow biopsy in non-Hodgkin lymphoma : A morphological study. In: Indian Journal of Pathology and Microbiology. 2009 ; Vol. 52, No. 3. pp. 332-338.
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abstract = "Context: Bone marrow (BM) biopsy is an integral part of staging work-up for non-Hodgkin lymphoma (NHL). Aims: To study the characteristics of BM involvement in NHL with respect to incidence, histologic pattern and morphology of infiltration and its discordance with the histology of primary anatomic site. Settings and Design: Forty-nine cases of NHL in which BM biopsy was performed for staging were included in this study, the primary site being classified according to the WHO classification for NHL. Materials and Methods: A prospective study of 49 cases was conducted. Bilateral BM biopsy was obtained from the posterior superior iliac spine. The biopsies were fixed in 10{\%} buffered formalin solution and decalcified using 10{\%} formal - formic acid for 4 - 6 h followed by routine processing. The serial sections were stained by hematoxylin and eosin and reticulin stains. Results: BM biopsy showed involvement by lymphoma in 27 cases (55.10{\%}). Unilateral positivity was found in four cases (14.81{\%} cases). The overall incidence of marrow involvement by NHL was 55.1{\%}. The incidence of involvement was higher in T-cell lymphomas when compared with B-cell lymphomas and predominant pattern of involvement was mixed. Diffuse large B-cell lymphomas had the lowest incidence in all the B-cell lymphomas. A discordant histology between BM and primary anatomic site was found in 29.63{\%} (8/27) of the cases, where it was seen more in follicular lymphomas and diffuse large B-cell lymphomas. Conclusions: Critical examination of BM biopsies can increase the diagnostic accuracy, thereby contributing to the prognosis and appropriate treatment modalities.",
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Bone marrow biopsy in non-Hodgkin lymphoma : A morphological study. / Kumar, Suneet; Rau, Aarathi R.; Naik, Ramadas; Kini, Hema; Mathai, Alka M.; Pai, Muktha R.; Khadilkar, Urmila N.

In: Indian Journal of Pathology and Microbiology, Vol. 52, No. 3, 01.07.2009, p. 332-338.

Research output: Contribution to journalArticle

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T1 - Bone marrow biopsy in non-Hodgkin lymphoma

T2 - A morphological study

AU - Kumar, Suneet

AU - Rau, Aarathi R.

AU - Naik, Ramadas

AU - Kini, Hema

AU - Mathai, Alka M.

AU - Pai, Muktha R.

AU - Khadilkar, Urmila N.

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N2 - Context: Bone marrow (BM) biopsy is an integral part of staging work-up for non-Hodgkin lymphoma (NHL). Aims: To study the characteristics of BM involvement in NHL with respect to incidence, histologic pattern and morphology of infiltration and its discordance with the histology of primary anatomic site. Settings and Design: Forty-nine cases of NHL in which BM biopsy was performed for staging were included in this study, the primary site being classified according to the WHO classification for NHL. Materials and Methods: A prospective study of 49 cases was conducted. Bilateral BM biopsy was obtained from the posterior superior iliac spine. The biopsies were fixed in 10% buffered formalin solution and decalcified using 10% formal - formic acid for 4 - 6 h followed by routine processing. The serial sections were stained by hematoxylin and eosin and reticulin stains. Results: BM biopsy showed involvement by lymphoma in 27 cases (55.10%). Unilateral positivity was found in four cases (14.81% cases). The overall incidence of marrow involvement by NHL was 55.1%. The incidence of involvement was higher in T-cell lymphomas when compared with B-cell lymphomas and predominant pattern of involvement was mixed. Diffuse large B-cell lymphomas had the lowest incidence in all the B-cell lymphomas. A discordant histology between BM and primary anatomic site was found in 29.63% (8/27) of the cases, where it was seen more in follicular lymphomas and diffuse large B-cell lymphomas. Conclusions: Critical examination of BM biopsies can increase the diagnostic accuracy, thereby contributing to the prognosis and appropriate treatment modalities.

AB - Context: Bone marrow (BM) biopsy is an integral part of staging work-up for non-Hodgkin lymphoma (NHL). Aims: To study the characteristics of BM involvement in NHL with respect to incidence, histologic pattern and morphology of infiltration and its discordance with the histology of primary anatomic site. Settings and Design: Forty-nine cases of NHL in which BM biopsy was performed for staging were included in this study, the primary site being classified according to the WHO classification for NHL. Materials and Methods: A prospective study of 49 cases was conducted. Bilateral BM biopsy was obtained from the posterior superior iliac spine. The biopsies were fixed in 10% buffered formalin solution and decalcified using 10% formal - formic acid for 4 - 6 h followed by routine processing. The serial sections were stained by hematoxylin and eosin and reticulin stains. Results: BM biopsy showed involvement by lymphoma in 27 cases (55.10%). Unilateral positivity was found in four cases (14.81% cases). The overall incidence of marrow involvement by NHL was 55.1%. The incidence of involvement was higher in T-cell lymphomas when compared with B-cell lymphomas and predominant pattern of involvement was mixed. Diffuse large B-cell lymphomas had the lowest incidence in all the B-cell lymphomas. A discordant histology between BM and primary anatomic site was found in 29.63% (8/27) of the cases, where it was seen more in follicular lymphomas and diffuse large B-cell lymphomas. Conclusions: Critical examination of BM biopsies can increase the diagnostic accuracy, thereby contributing to the prognosis and appropriate treatment modalities.

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