Caesalpinia bonduc linn extracts exhibit hepatoprotective effect on hepg2 cells against paracetamol by up-regulating glutathione related genes

Kumar Mr Bhat, Suhani Sumalatha, Nitesh Kumar, Divya Padma, K. Sreedhara Ranganath Pai, Shilpee Choudhary

Research output: Contribution to journalArticle

Abstract

Background: Caesalpinia bonduc (CB) is said to own various pharmacological and therapeutic application against a number of diseases. It is used widely in folk medicine to treat liver diseases. In the present study, we have made a sincere effort to evaluate the hepatoprotective activity of CB leaf extracts against Paracetamol (PCM) induced toxicity and their mechanism of hepatoprotection in the human Hepatocarcinoma cells (HepG2 cells) thereby providing scientific evidence for the same. Material and Methods: The hepatoprotective activity of CB was assessed in-vitro by the estimation of glutathione (GSH) and Malondialdehyde (MDA), anti-apoptotic assay/Annexin V and the expressions of genes such as Glutathione Reductase (GS-R) and Glutamate- Cysteine Ligase, Catalytic (GCLC). Results: The obtained results suggest that the aqueous extract of CB possess significant hepatoprotective activity. This activity may be due to the possible antioxidant property and the free radical scavenging ability of the extracts, which might clear the toxic metabolites of PCM. Conclusion: The present study suggests that the aqueous extract of CB have potential hepatoprotective activity, which may prevent the lipid peroxidation of the cell membrane by its antioxidant properties.

Original languageEnglish
Pages (from-to)1-12
Number of pages12
JournalJournal of Krishna Institute of Medical Sciences University
Volume8
Issue number2
Publication statusPublished - 01-04-2019

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Caesalpinia
Hep G2 Cells
Acetaminophen
Glutathione
Genes
Antioxidants
Glutamate-Cysteine Ligase
Glutathione Reductase
Poisons
Annexin A5
Traditional Medicine
Malondialdehyde
Lipid Peroxidation
Free Radicals
Liver Diseases
Cell Membrane
Pharmacology
Gene Expression

All Science Journal Classification (ASJC) codes

  • Medicine(all)

Cite this

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title = "Caesalpinia bonduc linn extracts exhibit hepatoprotective effect on hepg2 cells against paracetamol by up-regulating glutathione related genes",
abstract = "Background: Caesalpinia bonduc (CB) is said to own various pharmacological and therapeutic application against a number of diseases. It is used widely in folk medicine to treat liver diseases. In the present study, we have made a sincere effort to evaluate the hepatoprotective activity of CB leaf extracts against Paracetamol (PCM) induced toxicity and their mechanism of hepatoprotection in the human Hepatocarcinoma cells (HepG2 cells) thereby providing scientific evidence for the same. Material and Methods: The hepatoprotective activity of CB was assessed in-vitro by the estimation of glutathione (GSH) and Malondialdehyde (MDA), anti-apoptotic assay/Annexin V and the expressions of genes such as Glutathione Reductase (GS-R) and Glutamate- Cysteine Ligase, Catalytic (GCLC). Results: The obtained results suggest that the aqueous extract of CB possess significant hepatoprotective activity. This activity may be due to the possible antioxidant property and the free radical scavenging ability of the extracts, which might clear the toxic metabolites of PCM. Conclusion: The present study suggests that the aqueous extract of CB have potential hepatoprotective activity, which may prevent the lipid peroxidation of the cell membrane by its antioxidant properties.",
author = "Bhat, {Kumar Mr} and Suhani Sumalatha and Nitesh Kumar and Divya Padma and {Ranganath Pai}, {K. Sreedhara} and Shilpee Choudhary",
year = "2019",
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T1 - Caesalpinia bonduc linn extracts exhibit hepatoprotective effect on hepg2 cells against paracetamol by up-regulating glutathione related genes

AU - Bhat, Kumar Mr

AU - Sumalatha, Suhani

AU - Kumar, Nitesh

AU - Padma, Divya

AU - Ranganath Pai, K. Sreedhara

AU - Choudhary, Shilpee

PY - 2019/4/1

Y1 - 2019/4/1

N2 - Background: Caesalpinia bonduc (CB) is said to own various pharmacological and therapeutic application against a number of diseases. It is used widely in folk medicine to treat liver diseases. In the present study, we have made a sincere effort to evaluate the hepatoprotective activity of CB leaf extracts against Paracetamol (PCM) induced toxicity and their mechanism of hepatoprotection in the human Hepatocarcinoma cells (HepG2 cells) thereby providing scientific evidence for the same. Material and Methods: The hepatoprotective activity of CB was assessed in-vitro by the estimation of glutathione (GSH) and Malondialdehyde (MDA), anti-apoptotic assay/Annexin V and the expressions of genes such as Glutathione Reductase (GS-R) and Glutamate- Cysteine Ligase, Catalytic (GCLC). Results: The obtained results suggest that the aqueous extract of CB possess significant hepatoprotective activity. This activity may be due to the possible antioxidant property and the free radical scavenging ability of the extracts, which might clear the toxic metabolites of PCM. Conclusion: The present study suggests that the aqueous extract of CB have potential hepatoprotective activity, which may prevent the lipid peroxidation of the cell membrane by its antioxidant properties.

AB - Background: Caesalpinia bonduc (CB) is said to own various pharmacological and therapeutic application against a number of diseases. It is used widely in folk medicine to treat liver diseases. In the present study, we have made a sincere effort to evaluate the hepatoprotective activity of CB leaf extracts against Paracetamol (PCM) induced toxicity and their mechanism of hepatoprotection in the human Hepatocarcinoma cells (HepG2 cells) thereby providing scientific evidence for the same. Material and Methods: The hepatoprotective activity of CB was assessed in-vitro by the estimation of glutathione (GSH) and Malondialdehyde (MDA), anti-apoptotic assay/Annexin V and the expressions of genes such as Glutathione Reductase (GS-R) and Glutamate- Cysteine Ligase, Catalytic (GCLC). Results: The obtained results suggest that the aqueous extract of CB possess significant hepatoprotective activity. This activity may be due to the possible antioxidant property and the free radical scavenging ability of the extracts, which might clear the toxic metabolites of PCM. Conclusion: The present study suggests that the aqueous extract of CB have potential hepatoprotective activity, which may prevent the lipid peroxidation of the cell membrane by its antioxidant properties.

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