Can tissue PCR augment the diagnostic accuracy in genitourinary tract tuberculosis?

Arun Chawla, Kiran Chawla, Sreedhar Reddy, Nitika Arora, Indira Bairy, Sugandhi Rao, Padmaraj Hegde, Joseph Thomas

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Purpose: Conventional methods like smear and culture for Mycobacterium tuberculosis are of limited sensitivity and specificity. Histopathological examination (HPE) for the tissues obtained gives inconclusive diagnosis in the absence of caseous necrosis or stained acid-fast bacilli. This study was conducted to determine the utility of tissue PCR for diagnosing tuberculosis of the genitourinary tract (GUTB) and its comparative evaluation with HPE. Patients and Methods: A prospective study was conducted from January 2006 to August 2009 with 78 tissue specimens (renal, prostate, epididymis, penile and soft tissue) from patients with clinically suspected GUTB. All the samples were processed for both PCR and histopathology. Results: In 68 (87.1%) samples, results for both PCR and HPE were coinciding. False positivity and false negativity was observed in 5.1% (4/78) and 7.6% (6/78) samples, respectively. With HPE as the gold standard, PCR has shown sensitivity of 87.5% (95% CI 80.1; 91.9) and specificity of 86.7% (95% CI 74.9; 93.8) and positive agreement between two tests was observed as significant (0.7). PCR results were obtained within a mean period of 3.4 days while those of HPE were obtained in 7.2 days. Conclusions: Tissue PCR is a sensitive and specific method for obtaining early and timely diagnosis of GUTB. Application of tissue PCR results can augment the diagnostic accuracy in histopathologically labelled granulomatous inflammations.

Original languageEnglish
Pages (from-to)34-38
Number of pages5
JournalUrologia Internationalis
Volume88
Issue number1
DOIs
Publication statusPublished - 01-01-2012

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Tuberculosis
Polymerase Chain Reaction
Epididymis
Mycobacterium tuberculosis
Bacillus
Prostate
Early Diagnosis
Necrosis
Prospective Studies
Inflammation
Kidney
Sensitivity and Specificity
Acids

All Science Journal Classification (ASJC) codes

  • Urology

Cite this

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title = "Can tissue PCR augment the diagnostic accuracy in genitourinary tract tuberculosis?",
abstract = "Purpose: Conventional methods like smear and culture for Mycobacterium tuberculosis are of limited sensitivity and specificity. Histopathological examination (HPE) for the tissues obtained gives inconclusive diagnosis in the absence of caseous necrosis or stained acid-fast bacilli. This study was conducted to determine the utility of tissue PCR for diagnosing tuberculosis of the genitourinary tract (GUTB) and its comparative evaluation with HPE. Patients and Methods: A prospective study was conducted from January 2006 to August 2009 with 78 tissue specimens (renal, prostate, epididymis, penile and soft tissue) from patients with clinically suspected GUTB. All the samples were processed for both PCR and histopathology. Results: In 68 (87.1{\%}) samples, results for both PCR and HPE were coinciding. False positivity and false negativity was observed in 5.1{\%} (4/78) and 7.6{\%} (6/78) samples, respectively. With HPE as the gold standard, PCR has shown sensitivity of 87.5{\%} (95{\%} CI 80.1; 91.9) and specificity of 86.7{\%} (95{\%} CI 74.9; 93.8) and positive agreement between two tests was observed as significant (0.7). PCR results were obtained within a mean period of 3.4 days while those of HPE were obtained in 7.2 days. Conclusions: Tissue PCR is a sensitive and specific method for obtaining early and timely diagnosis of GUTB. Application of tissue PCR results can augment the diagnostic accuracy in histopathologically labelled granulomatous inflammations.",
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Can tissue PCR augment the diagnostic accuracy in genitourinary tract tuberculosis? / Chawla, Arun; Chawla, Kiran; Reddy, Sreedhar; Arora, Nitika; Bairy, Indira; Rao, Sugandhi; Hegde, Padmaraj; Thomas, Joseph.

In: Urologia Internationalis, Vol. 88, No. 1, 01.01.2012, p. 34-38.

Research output: Contribution to journalArticle

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AU - Chawla, Arun

AU - Chawla, Kiran

AU - Reddy, Sreedhar

AU - Arora, Nitika

AU - Bairy, Indira

AU - Rao, Sugandhi

AU - Hegde, Padmaraj

AU - Thomas, Joseph

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N2 - Purpose: Conventional methods like smear and culture for Mycobacterium tuberculosis are of limited sensitivity and specificity. Histopathological examination (HPE) for the tissues obtained gives inconclusive diagnosis in the absence of caseous necrosis or stained acid-fast bacilli. This study was conducted to determine the utility of tissue PCR for diagnosing tuberculosis of the genitourinary tract (GUTB) and its comparative evaluation with HPE. Patients and Methods: A prospective study was conducted from January 2006 to August 2009 with 78 tissue specimens (renal, prostate, epididymis, penile and soft tissue) from patients with clinically suspected GUTB. All the samples were processed for both PCR and histopathology. Results: In 68 (87.1%) samples, results for both PCR and HPE were coinciding. False positivity and false negativity was observed in 5.1% (4/78) and 7.6% (6/78) samples, respectively. With HPE as the gold standard, PCR has shown sensitivity of 87.5% (95% CI 80.1; 91.9) and specificity of 86.7% (95% CI 74.9; 93.8) and positive agreement between two tests was observed as significant (0.7). PCR results were obtained within a mean period of 3.4 days while those of HPE were obtained in 7.2 days. Conclusions: Tissue PCR is a sensitive and specific method for obtaining early and timely diagnosis of GUTB. Application of tissue PCR results can augment the diagnostic accuracy in histopathologically labelled granulomatous inflammations.

AB - Purpose: Conventional methods like smear and culture for Mycobacterium tuberculosis are of limited sensitivity and specificity. Histopathological examination (HPE) for the tissues obtained gives inconclusive diagnosis in the absence of caseous necrosis or stained acid-fast bacilli. This study was conducted to determine the utility of tissue PCR for diagnosing tuberculosis of the genitourinary tract (GUTB) and its comparative evaluation with HPE. Patients and Methods: A prospective study was conducted from January 2006 to August 2009 with 78 tissue specimens (renal, prostate, epididymis, penile and soft tissue) from patients with clinically suspected GUTB. All the samples were processed for both PCR and histopathology. Results: In 68 (87.1%) samples, results for both PCR and HPE were coinciding. False positivity and false negativity was observed in 5.1% (4/78) and 7.6% (6/78) samples, respectively. With HPE as the gold standard, PCR has shown sensitivity of 87.5% (95% CI 80.1; 91.9) and specificity of 86.7% (95% CI 74.9; 93.8) and positive agreement between two tests was observed as significant (0.7). PCR results were obtained within a mean period of 3.4 days while those of HPE were obtained in 7.2 days. Conclusions: Tissue PCR is a sensitive and specific method for obtaining early and timely diagnosis of GUTB. Application of tissue PCR results can augment the diagnostic accuracy in histopathologically labelled granulomatous inflammations.

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