Carcinogenesis in human skin grafted to SCID mice

Peter W. Soballe, Kathleen T. Montane, Kapaettu Satyamoorthy, Mark Nesbit, Meenhard Herlyn

    Research output: Contribution to journalArticle

    33 Citations (Scopus)

    Abstract

    To directly examine a multistage carcinogenesis model and the role of UV light in human tissues, we grafted human skin unto mice with severe combined immunodeficiency disease. We found that the maximum dose of UV radiation in the B range (UVB; 280-320 nm) tolerated by these grafts was 500 J/m2 three times weekly. One hundred fifty-one grafted mice were then randomized and observed for a median of 9 months in five groups: no treatment, chemical initiation alone, UVB as a complete carcinogen, initiation plus UVB promotion, and initiation plus UVB and phorbol ester promotion. Actinic damage and squamous atypia were found in grafts of all groups receiving UV treatment; unequivocal human squamous carcinomas developed in two of these. Species origin was verified by human-specific bisbenzimide staining and in situ hybridization for human-specific Alu segment. Overall basal proliferation, measured immunohistologically, was reduced in UV-treated grafts, but loci of hyperproliferation were seen in conjunction with the dedifferentiated expression of cytokeratins 1, 10 and 5, 8. Murine tumors also developed frequently, confirming the biological relevance of the carcinogenic strategies tested. These findings demonstrate that development of malignant human tumors can be experimentally accelerated in human tissue.

    Original languageEnglish
    Pages (from-to)757-764
    Number of pages8
    JournalCancer Research
    Volume56
    Issue number4
    Publication statusPublished - 15-02-1996

    Fingerprint

    SCID Mice
    Carcinogenesis
    Skin
    Transplants
    Bisbenzimidazole
    Severe Combined Immunodeficiency
    Human Development
    Phorbol Esters
    Ultraviolet Rays
    Keratins
    Carcinogens
    In Situ Hybridization
    Squamous Cell Carcinoma
    Neoplasms
    Radiation
    Staining and Labeling

    All Science Journal Classification (ASJC) codes

    • Cancer Research
    • Oncology

    Cite this

    Soballe, P. W., Montane, K. T., Satyamoorthy, K., Nesbit, M., & Herlyn, M. (1996). Carcinogenesis in human skin grafted to SCID mice. Cancer Research, 56(4), 757-764.
    Soballe, Peter W. ; Montane, Kathleen T. ; Satyamoorthy, Kapaettu ; Nesbit, Mark ; Herlyn, Meenhard. / Carcinogenesis in human skin grafted to SCID mice. In: Cancer Research. 1996 ; Vol. 56, No. 4. pp. 757-764.
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    Soballe, PW, Montane, KT, Satyamoorthy, K, Nesbit, M & Herlyn, M 1996, 'Carcinogenesis in human skin grafted to SCID mice', Cancer Research, vol. 56, no. 4, pp. 757-764.

    Carcinogenesis in human skin grafted to SCID mice. / Soballe, Peter W.; Montane, Kathleen T.; Satyamoorthy, Kapaettu; Nesbit, Mark; Herlyn, Meenhard.

    In: Cancer Research, Vol. 56, No. 4, 15.02.1996, p. 757-764.

    Research output: Contribution to journalArticle

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    Soballe PW, Montane KT, Satyamoorthy K, Nesbit M, Herlyn M. Carcinogenesis in human skin grafted to SCID mice. Cancer Research. 1996 Feb 15;56(4):757-764.