Catalytic activity of Peptidase N is required for adaptation of Escherichia coli to nutritional downshift and high temperature stress

Manoj Bhosale, Anujith Kumar, Mrinmoy Das, Chetana Bhaskarla, Vikas Agarwal, Dipankar Nandi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Peptidase N (PepN), the sole M1 family member in Escherichia coli, displays broad substrate specificity and modulates stress responses: it lowers resistance to sodium salicylate (NaSal)-induced stress but is required during nutritional downshift and high temperature (NDHT) stress. The expression of PepN does not significantly change during different growth phases in LB or NaSal-induced stress; however, PepN amounts are lower during NDHT stress. To gain mechanistic insights on the roles of catalytic activity of PepN in modulating these two stress responses, alanine mutants of PepN replacing E264 (GAMEN motif) and E298 (HEXXH motif) were generated. There are no major structural changes between purified wild type (WT) and mutant proteins, which are catalytically inactive. Importantly, growth profiles of Δ pepN upon expression of WT or mutant proteins demonstrated the importance of catalytic activity during NDHT but not NaSal-induced stress. Further fluorescamine reactivity studies demonstrated that the catalytic activity of PepN is required to generate higher intracellular amounts of free N-terminal amino acids; consequently, the lower growth of Δ pepN during NDHT stress increases with high amounts of casamino acids. Together, this study sheds insights on the expression and functional roles of the catalytic activity of PepN during adaptation to NDHT stress.

Original languageEnglish
Pages (from-to)56-64
Number of pages9
JournalMicrobiological Research
Volume168
Issue number1
DOIs
Publication statusPublished - 15-01-2013

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Escherichia coli
Temperature
Nose
Mutant Proteins
Growth
Fluorescamine
Sodium Salicylate
Substrate Specificity
peptidase N
Alanine
Amino Acids

All Science Journal Classification (ASJC) codes

  • Microbiology

Cite this

Bhosale, Manoj ; Kumar, Anujith ; Das, Mrinmoy ; Bhaskarla, Chetana ; Agarwal, Vikas ; Nandi, Dipankar. / Catalytic activity of Peptidase N is required for adaptation of Escherichia coli to nutritional downshift and high temperature stress. In: Microbiological Research. 2013 ; Vol. 168, No. 1. pp. 56-64.
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Catalytic activity of Peptidase N is required for adaptation of Escherichia coli to nutritional downshift and high temperature stress. / Bhosale, Manoj; Kumar, Anujith; Das, Mrinmoy; Bhaskarla, Chetana; Agarwal, Vikas; Nandi, Dipankar.

In: Microbiological Research, Vol. 168, No. 1, 15.01.2013, p. 56-64.

Research output: Contribution to journalArticle

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