Changing antibiotic susceptibility pattern in uropathogenic escherichia coli over a period of 5 years in a tertiary care center

Shobha Prasada, Archana Bhat, Sevitha Bhat, Shalini Shenoy Mulki, Sanyuktha Tulasidas

Research output: Contribution to journalArticle

Abstract

Objective: To assess the changing antibiotic sensitivity pattern in Uropathogenic E. coli over a period of time (2013–2017) with a special emphasis on ESBL-producing E. coli. Methods: This retrospective study was carried out in the Department of Microbiology, Kasturba Medical College, Ambedkar Circle, Mangalore. A retrospective time bound analysis of 500 samples/year was performed. The urine samples received from the suspected cases of urinary tract infection (UTI) were processed. Wet mount examinations of urine samples were done. The urine culture was done by a semi-quantitative method on Mac Conkey’s agar, Cysteine Lactose Electrolyte Deficient (CLED) medium, and UTI Chrome agar. Culture plates were incubated for 18–24 hours at 37°C. Urine samples with a colony count of ≥105 CFU/ml were considered significant. The uropathogens were identified by their biochemical reactions. The antibiotic susceptibility testing (AST) was carried out using a Vitek Compact 2 system and Modified Kirby Bauer disc diffusion method. Results: Antibiotic resistance of Uropathogenic E.coli to cephalosporins increased from 51 to 58%, Cotrimoxazole: 52 to 59%, Piperacillin tazobactam 9.4 to 23%, Carbapenems 0 to 5.9%. Antibiotic resistance to netilmicin has reduced from 8 to 6.5%, and norfloxacin 59 to 48%. The rates of ESBL production have increased from 45.2 to 59.6% in the 5 years. Conclusion: The increasing antibiotic resistance trends in UTI patients indicate that it is vitally important to use them conservatively. Proper guidelines, management of antibiotic usage, and constant information to the clinicians regarding the sensitivity pattern can help to prevent drug resistance.

Original languageEnglish
Pages (from-to)1439-1443
Number of pages5
JournalInfection and Drug Resistance
Volume12
DOIs
Publication statusPublished - 01-01-2019

Fingerprint

Uropathogenic Escherichia coli
Tertiary Care Centers
Microbial Drug Resistance
Urinary Tract Infections
Urine
Anti-Bacterial Agents
Agar
Disk Diffusion Antimicrobial Tests
Netilmicin
Norfloxacin
Carbapenems
Sulfamethoxazole Drug Combination Trimethoprim
Cephalosporins
Lactose
Microbiology
Drug Resistance
Electrolytes
Cysteine
Retrospective Studies
Guidelines

All Science Journal Classification (ASJC) codes

  • Pharmacology
  • Infectious Diseases
  • Pharmacology (medical)

Cite this

Prasada, Shobha ; Bhat, Archana ; Bhat, Sevitha ; Mulki, Shalini Shenoy ; Tulasidas, Sanyuktha. / Changing antibiotic susceptibility pattern in uropathogenic escherichia coli over a period of 5 years in a tertiary care center. In: Infection and Drug Resistance. 2019 ; Vol. 12. pp. 1439-1443.
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abstract = "Objective: To assess the changing antibiotic sensitivity pattern in Uropathogenic E. coli over a period of time (2013–2017) with a special emphasis on ESBL-producing E. coli. Methods: This retrospective study was carried out in the Department of Microbiology, Kasturba Medical College, Ambedkar Circle, Mangalore. A retrospective time bound analysis of 500 samples/year was performed. The urine samples received from the suspected cases of urinary tract infection (UTI) were processed. Wet mount examinations of urine samples were done. The urine culture was done by a semi-quantitative method on Mac Conkey’s agar, Cysteine Lactose Electrolyte Deficient (CLED) medium, and UTI Chrome agar. Culture plates were incubated for 18–24 hours at 37°C. Urine samples with a colony count of ≥105 CFU/ml were considered significant. The uropathogens were identified by their biochemical reactions. The antibiotic susceptibility testing (AST) was carried out using a Vitek Compact 2 system and Modified Kirby Bauer disc diffusion method. Results: Antibiotic resistance of Uropathogenic E.coli to cephalosporins increased from 51 to 58{\%}, Cotrimoxazole: 52 to 59{\%}, Piperacillin tazobactam 9.4 to 23{\%}, Carbapenems 0 to 5.9{\%}. Antibiotic resistance to netilmicin has reduced from 8 to 6.5{\%}, and norfloxacin 59 to 48{\%}. The rates of ESBL production have increased from 45.2 to 59.6{\%} in the 5 years. Conclusion: The increasing antibiotic resistance trends in UTI patients indicate that it is vitally important to use them conservatively. Proper guidelines, management of antibiotic usage, and constant information to the clinicians regarding the sensitivity pattern can help to prevent drug resistance.",
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Changing antibiotic susceptibility pattern in uropathogenic escherichia coli over a period of 5 years in a tertiary care center. / Prasada, Shobha; Bhat, Archana; Bhat, Sevitha; Mulki, Shalini Shenoy; Tulasidas, Sanyuktha.

In: Infection and Drug Resistance, Vol. 12, 01.01.2019, p. 1439-1443.

Research output: Contribution to journalArticle

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