Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity

S. Khan, H.A. Pradeep, B.S. Ashok Kumar, M. Anil Kumar, S. Manimaran, R. Chandrasekhar, P. Vijayan

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Abstract

This study was undertaken to investigate the chemoprotective activity of ethanol extract of the bark of Anogeissus latifolia (ALE) against Paracetamol induced hepatotoxicity in rat model. The ethanol extract of ALE at 100 mg and 300 mg/kg and Liv-52 (a reference heptoprotective agent) at 2 ml/kg for seven days. The effect of extract on serum amino transferase (ALAT), serum aspartate aminotransferase (ASAT) alkaline phospate (ALP) and histopathology were studied. Ethanol extract of ALE showed a dose dependant significant reduction in the levels of ASAT, ALAT and ALP that was altered because of paracetamol intoxication in rats. Histopathological observations confirmed the beneficial roles of ALE and Liv-52. The ethanol extract of ALE was also investigated for antioxidant activity; by lipid peroxidation method. From these results, it was suggested that extract of ALE colud protect from paracetamol-induced Heptotoxicity by its antioxidative effects.
Original languageEnglish
Pages (from-to)303-327
Number of pages25
JournalPharmacologyonline
Volume2
Publication statusPublished - 2008

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Acetaminophen
Ethanol
Aspartate Aminotransferases
Transferases
Serum
Lipid Peroxidation
Antioxidants
Liv 52

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Khan, S., Pradeep, H. A., Ashok Kumar, B. S., Anil Kumar, M., Manimaran, S., Chandrasekhar, R., & Vijayan, P. (2008). Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity. Pharmacologyonline, 2, 303-327.
Khan, S. ; Pradeep, H.A. ; Ashok Kumar, B.S. ; Anil Kumar, M. ; Manimaran, S. ; Chandrasekhar, R. ; Vijayan, P. / Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity. In: Pharmacologyonline. 2008 ; Vol. 2. pp. 303-327.
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title = "Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity",
abstract = "This study was undertaken to investigate the chemoprotective activity of ethanol extract of the bark of Anogeissus latifolia (ALE) against Paracetamol induced hepatotoxicity in rat model. The ethanol extract of ALE at 100 mg and 300 mg/kg and Liv-52 (a reference heptoprotective agent) at 2 ml/kg for seven days. The effect of extract on serum amino transferase (ALAT), serum aspartate aminotransferase (ASAT) alkaline phospate (ALP) and histopathology were studied. Ethanol extract of ALE showed a dose dependant significant reduction in the levels of ASAT, ALAT and ALP that was altered because of paracetamol intoxication in rats. Histopathological observations confirmed the beneficial roles of ALE and Liv-52. The ethanol extract of ALE was also investigated for antioxidant activity; by lipid peroxidation method. From these results, it was suggested that extract of ALE colud protect from paracetamol-induced Heptotoxicity by its antioxidative effects.",
author = "S. Khan and H.A. Pradeep and {Ashok Kumar}, B.S. and {Anil Kumar}, M. and S. Manimaran and R. Chandrasekhar and P. Vijayan",
note = "Cited By :4 Export Date: 10 November 2017 Correspondence Address: Khan, S.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Udupi Dist. Karnataka, India; email: saleem_jss@hotmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; liv 52, 54847-94-8; paracetamol, 103-90-2 Tradenames: liv 52 Manufacturers: Himalaya, India References: (1985) Pharmacognosy of Indigenous drugs, , Central council for Research in Ayurveda and Siddha, New Delhi, Anonymous; Atawodi, S.E., Antioxidant potentials of African plants (2005) Afr. J. Biotechnol, 4, pp. 128-133; Bhakuni, D.S., Gupta, B., Dhar, M.L., Srimal, R.C., Screening of Indian Plants for Biological activity (1971) Ind. J. Expt.Biol, 9, p. 91; Deshpande, V.H., Patil, A.D., Rama Roa, A.V., and Venkataraman, Methylellagic acid and methylflavellagic acid from Anogeissus latifolia bark, Ind. J. Chem. 1976:14B: 641-643; Dixon, M.F., Nimmo, J., Prescott, L.F., Experimental paracetamol induced hepatic necrosis: A histopathological study (1971) J. Pathol, 103, pp. 225-227; Gupta, M., Mazumder, U.K., Sambath Kumar, R., Antioxidant and protective effects of Ervatamia coronaria Stapf., leaves against CCl4-induced liver damage (2004) Euro. Bul. Drug. Res, 12, pp. 13-22; Gupta, M., Mazumder, U.K., Siva kumar, T., Antioxidant and Hepatoprotective effects of Bauhinia racemosa against Paracetamol and CCl4-induced liver damage in rats, Iran (2004) J. Pharmacol. Therapeutics, 3, pp. 12-20; Hong, R.W., Rounds, J.D., Helton, W.S., Robinson, M.K., Wilmore, D.W., Glutamine preserves liver glutathione after lethal hepatic injury (1992) Ann. Surg, 215, pp. 114-119; Khalid, H., Janbaz Sheikh, A., Saeed Anwar, H., Gilani, Protective effect of Rutin on Paracetamol and CCl4-induced hepatotoxicity in rodents (2002) Fitoterapia, 73, pp. 557-563; Kukongviriyapan, V., Janyacharoen, T., Kukongviriyapan, U., Hepatoprotective and antioxidant activities of Tetracera loureiri (2003) Phytother. Res, 17, pp. 717-721; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 265-275; Mitra, S.K., Seshadri, S.J., Venkataranganna, M.V., Gopumadhavan, S., Udupa, V., Sarma, D.N.K., Effect of HD-03 - A herbal formulation in galactosamine induced hepatotoxicity in rats (2000) Ind. J. Physiol. Pharmacol, 44, pp. 82-86; Mukherjee, K.L., (1988) Medical Laboratory Technology, , Tata Mc-Graw Hill Publication Company, New Delhi; Niehaus, W.G., Samuelson, B., Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation (1968) Eur. J. Biochem, 17, pp. 126-130; Pushpangadan, Annie Shirwaikar, Govindarajan, and Rawat, A.K.S., Antiulcer and antimicrobial activity of Anogeissus latifolia. J. Ethno. Pharmacol. 2006: 106: 57-61; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava ( Anogeissus latifolia) Tannins: Part 3-Polyphenols of Bark, Sapwood and Heartwood of Dhava (1965) Ind. J. Chem, 3, pp. 308-310; Schiodt, F.V., Atillasoy, E., Shakil, A.O., Etiology and outcome for 295 patients with acute liver failure in the United States (1999) Liver. Transplant. Surg, 5, pp. 29-34; Mehrotra, S., Shirwaikar, A., Govindarajan, Pushpangadan, Antioxidant potential of Anogeissus latifolia (2004) Biol.Pharm. Bull, 27 (8), pp. 1266-1269; Whitcomb, D.C., Block, G.D., Association of acetaminophen hepatotoxicity with fasting and ethanol use (1994) JAMA, 272, pp. 1845-1850; Zimmerman, H.J., Seeff, L.B., Enzymes in hepatic disease, in Goodly (1970) Diagnostic Enzmology, pp. 1-38. , E.L. ed, Lea and Febiger, Philadelphia, USA",
year = "2008",
language = "English",
volume = "2",
pages = "303--327",
journal = "Pharmacologyonline",
issn = "1827-8620",
publisher = "SILAE (Italo-Latin American Society of Ethnomedicine)",

}

Khan, S, Pradeep, HA, Ashok Kumar, BS, Anil Kumar, M, Manimaran, S, Chandrasekhar, R & Vijayan, P 2008, 'Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity', Pharmacologyonline, vol. 2, pp. 303-327.

Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity. / Khan, S.; Pradeep, H.A.; Ashok Kumar, B.S.; Anil Kumar, M.; Manimaran, S.; Chandrasekhar, R.; Vijayan, P.

In: Pharmacologyonline, Vol. 2, 2008, p. 303-327.

Research output: Contribution to journalArticle

TY - JOUR

T1 - Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity

AU - Khan, S.

AU - Pradeep, H.A.

AU - Ashok Kumar, B.S.

AU - Anil Kumar, M.

AU - Manimaran, S.

AU - Chandrasekhar, R.

AU - Vijayan, P.

N1 - Cited By :4 Export Date: 10 November 2017 Correspondence Address: Khan, S.; Department of Pharmacognosy, Manipal College of Pharmaceutical Sciences, Manipal, Udupi Dist. Karnataka, India; email: saleem_jss@hotmail.com Chemicals/CAS: alanine aminotransferase, 9000-86-6, 9014-30-6; alcohol, 64-17-5; alkaline phosphatase, 9001-78-9; aspartate aminotransferase, 9000-97-9; liv 52, 54847-94-8; paracetamol, 103-90-2 Tradenames: liv 52 Manufacturers: Himalaya, India References: (1985) Pharmacognosy of Indigenous drugs, , Central council for Research in Ayurveda and Siddha, New Delhi, Anonymous; Atawodi, S.E., Antioxidant potentials of African plants (2005) Afr. J. Biotechnol, 4, pp. 128-133; Bhakuni, D.S., Gupta, B., Dhar, M.L., Srimal, R.C., Screening of Indian Plants for Biological activity (1971) Ind. J. Expt.Biol, 9, p. 91; Deshpande, V.H., Patil, A.D., Rama Roa, A.V., and Venkataraman, Methylellagic acid and methylflavellagic acid from Anogeissus latifolia bark, Ind. J. Chem. 1976:14B: 641-643; Dixon, M.F., Nimmo, J., Prescott, L.F., Experimental paracetamol induced hepatic necrosis: A histopathological study (1971) J. Pathol, 103, pp. 225-227; Gupta, M., Mazumder, U.K., Sambath Kumar, R., Antioxidant and protective effects of Ervatamia coronaria Stapf., leaves against CCl4-induced liver damage (2004) Euro. Bul. Drug. Res, 12, pp. 13-22; Gupta, M., Mazumder, U.K., Siva kumar, T., Antioxidant and Hepatoprotective effects of Bauhinia racemosa against Paracetamol and CCl4-induced liver damage in rats, Iran (2004) J. Pharmacol. Therapeutics, 3, pp. 12-20; Hong, R.W., Rounds, J.D., Helton, W.S., Robinson, M.K., Wilmore, D.W., Glutamine preserves liver glutathione after lethal hepatic injury (1992) Ann. Surg, 215, pp. 114-119; Khalid, H., Janbaz Sheikh, A., Saeed Anwar, H., Gilani, Protective effect of Rutin on Paracetamol and CCl4-induced hepatotoxicity in rodents (2002) Fitoterapia, 73, pp. 557-563; Kukongviriyapan, V., Janyacharoen, T., Kukongviriyapan, U., Hepatoprotective and antioxidant activities of Tetracera loureiri (2003) Phytother. Res, 17, pp. 717-721; Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J., Protein measurement with the Folin phenol reagent (1951) J. Biol. Chem, 193, pp. 265-275; Mitra, S.K., Seshadri, S.J., Venkataranganna, M.V., Gopumadhavan, S., Udupa, V., Sarma, D.N.K., Effect of HD-03 - A herbal formulation in galactosamine induced hepatotoxicity in rats (2000) Ind. J. Physiol. Pharmacol, 44, pp. 82-86; Mukherjee, K.L., (1988) Medical Laboratory Technology, , Tata Mc-Graw Hill Publication Company, New Delhi; Niehaus, W.G., Samuelson, B., Formation of malondialdehyde from phospholipids arachidonate during microsomal lipid peroxidation (1968) Eur. J. Biochem, 17, pp. 126-130; Pushpangadan, Annie Shirwaikar, Govindarajan, and Rawat, A.K.S., Antiulcer and antimicrobial activity of Anogeissus latifolia. J. Ethno. Pharmacol. 2006: 106: 57-61; Reddy, K.K., Rajadurai, S., Nayudamma, Y., Studies on Dhava ( Anogeissus latifolia) Tannins: Part 3-Polyphenols of Bark, Sapwood and Heartwood of Dhava (1965) Ind. J. Chem, 3, pp. 308-310; Schiodt, F.V., Atillasoy, E., Shakil, A.O., Etiology and outcome for 295 patients with acute liver failure in the United States (1999) Liver. Transplant. Surg, 5, pp. 29-34; Mehrotra, S., Shirwaikar, A., Govindarajan, Pushpangadan, Antioxidant potential of Anogeissus latifolia (2004) Biol.Pharm. Bull, 27 (8), pp. 1266-1269; Whitcomb, D.C., Block, G.D., Association of acetaminophen hepatotoxicity with fasting and ethanol use (1994) JAMA, 272, pp. 1845-1850; Zimmerman, H.J., Seeff, L.B., Enzymes in hepatic disease, in Goodly (1970) Diagnostic Enzmology, pp. 1-38. , E.L. ed, Lea and Febiger, Philadelphia, USA

PY - 2008

Y1 - 2008

N2 - This study was undertaken to investigate the chemoprotective activity of ethanol extract of the bark of Anogeissus latifolia (ALE) against Paracetamol induced hepatotoxicity in rat model. The ethanol extract of ALE at 100 mg and 300 mg/kg and Liv-52 (a reference heptoprotective agent) at 2 ml/kg for seven days. The effect of extract on serum amino transferase (ALAT), serum aspartate aminotransferase (ASAT) alkaline phospate (ALP) and histopathology were studied. Ethanol extract of ALE showed a dose dependant significant reduction in the levels of ASAT, ALAT and ALP that was altered because of paracetamol intoxication in rats. Histopathological observations confirmed the beneficial roles of ALE and Liv-52. The ethanol extract of ALE was also investigated for antioxidant activity; by lipid peroxidation method. From these results, it was suggested that extract of ALE colud protect from paracetamol-induced Heptotoxicity by its antioxidative effects.

AB - This study was undertaken to investigate the chemoprotective activity of ethanol extract of the bark of Anogeissus latifolia (ALE) against Paracetamol induced hepatotoxicity in rat model. The ethanol extract of ALE at 100 mg and 300 mg/kg and Liv-52 (a reference heptoprotective agent) at 2 ml/kg for seven days. The effect of extract on serum amino transferase (ALAT), serum aspartate aminotransferase (ASAT) alkaline phospate (ALP) and histopathology were studied. Ethanol extract of ALE showed a dose dependant significant reduction in the levels of ASAT, ALAT and ALP that was altered because of paracetamol intoxication in rats. Histopathological observations confirmed the beneficial roles of ALE and Liv-52. The ethanol extract of ALE was also investigated for antioxidant activity; by lipid peroxidation method. From these results, it was suggested that extract of ALE colud protect from paracetamol-induced Heptotoxicity by its antioxidative effects.

M3 - Article

VL - 2

SP - 303

EP - 327

JO - Pharmacologyonline

JF - Pharmacologyonline

SN - 1827-8620

ER -

Khan S, Pradeep HA, Ashok Kumar BS, Anil Kumar M, Manimaran S, Chandrasekhar R et al. Chemoprotective effect of Anogeissus latifolia bark extract on paracetamol-induced hepatotoxicity. Pharmacologyonline. 2008;2:303-327.