Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

Mahadev Rao, Bradley A. Carlson, Sergey V. Novoselov, Donald P. Weeks, Vadim N. Gladyshev, Dolph L. Hatfield

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec TRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

Original languageEnglish
Pages (from-to)923-930
Number of pages8
JournalRNA
Volume9
Issue number8
DOIs
Publication statusPublished - 01-08-2003

Fingerprint

Selenocysteine
Chlamydomonas reinhardtii
Serine-tRNA Ligase
Archaea
Transfer RNA
selenocysteinyl-tRNA
Eukaryota
Serine
Proteins
Nucleotides
Bacteria
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

Cite this

Rao, M., Carlson, B. A., Novoselov, S. V., Weeks, D. P., Gladyshev, V. N., & Hatfield, D. L. (2003). Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec. RNA, 9(8), 923-930. https://doi.org/10.1261/rna.5510503
Rao, Mahadev ; Carlson, Bradley A. ; Novoselov, Sergey V. ; Weeks, Donald P. ; Gladyshev, Vadim N. ; Hatfield, Dolph L. / Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec. In: RNA. 2003 ; Vol. 9, No. 8. pp. 923-930.
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Rao, M, Carlson, BA, Novoselov, SV, Weeks, DP, Gladyshev, VN & Hatfield, DL 2003, 'Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec', RNA, vol. 9, no. 8, pp. 923-930. https://doi.org/10.1261/rna.5510503

Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec. / Rao, Mahadev; Carlson, Bradley A.; Novoselov, Sergey V.; Weeks, Donald P.; Gladyshev, Vadim N.; Hatfield, Dolph L.

In: RNA, Vol. 9, No. 8, 01.08.2003, p. 923-930.

Research output: Contribution to journalArticle

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Rao M, Carlson BA, Novoselov SV, Weeks DP, Gladyshev VN, Hatfield DL. Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec. RNA. 2003 Aug 1;9(8):923-930. https://doi.org/10.1261/rna.5510503