Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec

Mahadev Rao, Bradley A. Carlson, Sergey V. Novoselov, Donald P. Weeks, Vadim N. Gladyshev, Dolph L. Hatfield

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29 Citations (Scopus)

Abstract

Eukaryotic selenocysteine (Sec) protein insertion machinery was thought to be restricted to animals, but the occurrence of both Sec-containing proteins and the Sec insertion system was recently found in Chlamydomonas reinhardtii, a member of the plant kingdom. Herein, we used RT-PCR to determine the sequence of C. reinhardtii Sec tRNA[Ser]Sec, the first non-animal eukaryotic Sec tRNA[Ser]Sec sequence. Like its animal counterpart, it is 90 nucleotides in length, is aminoacylated with serine by seryl-tRNA synthetase, and decodes specifically UGA. Evolutionary analyses of known Sec tRNAs identify the C. reinhardtii form as the most diverged eukaryotic Sec TRNA[Ser]Sec and reveal a common origin for this tRNA in bacteria, archaea, and eukaryotes.

Original languageEnglish
Pages (from-to)923-930
Number of pages8
JournalRNA
Volume9
Issue number8
DOIs
Publication statusPublished - 01-08-2003

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology

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    Rao, M., Carlson, B. A., Novoselov, S. V., Weeks, D. P., Gladyshev, V. N., & Hatfield, D. L. (2003). Chlamydomonas reinhardtii selenocysteine tRNA[Ser]Sec. RNA, 9(8), 923-930. https://doi.org/10.1261/rna.5510503