Chlortetracycline, a fluorescent probe for pH of calcium stores in cells

Jose Jacob, Deepthi Chandran, Ranjana Sasidharan, Leena Kuruvila, U. K. Madhusudan, Nivedita L. Rao, Dibyajyoti Banerjee

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.

Original languageEnglish
Pages (from-to)671-674
Number of pages4
JournalCurrent Science
Volume84
Issue number5
Publication statusPublished - 10-03-2003
Externally publishedYes

Fingerprint

Chlortetracycline
chlortetracycline
Fluorescent Dyes
Calcium
calcium
Nigericin
cells
Ionophores
nigericin
Endoplasmic Reticulum
Buffers
ionophores
Lymphocytes
endoplasmic reticulum
lymphocytes
buffers
blood

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)
  • General

Cite this

Jacob, J., Chandran, D., Sasidharan, R., Kuruvila, L., Madhusudan, U. K., Rao, N. L., & Banerjee, D. (2003). Chlortetracycline, a fluorescent probe for pH of calcium stores in cells. Current Science, 84(5), 671-674.
Jacob, Jose ; Chandran, Deepthi ; Sasidharan, Ranjana ; Kuruvila, Leena ; Madhusudan, U. K. ; Rao, Nivedita L. ; Banerjee, Dibyajyoti. / Chlortetracycline, a fluorescent probe for pH of calcium stores in cells. In: Current Science. 2003 ; Vol. 84, No. 5. pp. 671-674.
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Jacob, J, Chandran, D, Sasidharan, R, Kuruvila, L, Madhusudan, UK, Rao, NL & Banerjee, D 2003, 'Chlortetracycline, a fluorescent probe for pH of calcium stores in cells', Current Science, vol. 84, no. 5, pp. 671-674.

Chlortetracycline, a fluorescent probe for pH of calcium stores in cells. / Jacob, Jose; Chandran, Deepthi; Sasidharan, Ranjana; Kuruvila, Leena; Madhusudan, U. K.; Rao, Nivedita L.; Banerjee, Dibyajyoti.

In: Current Science, Vol. 84, No. 5, 10.03.2003, p. 671-674.

Research output: Contribution to journalArticle

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AU - Jacob, Jose

AU - Chandran, Deepthi

AU - Sasidharan, Ranjana

AU - Kuruvila, Leena

AU - Madhusudan, U. K.

AU - Rao, Nivedita L.

AU - Banerjee, Dibyajyoti

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AB - Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.

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Jacob J, Chandran D, Sasidharan R, Kuruvila L, Madhusudan UK, Rao NL et al. Chlortetracycline, a fluorescent probe for pH of calcium stores in cells. Current Science. 2003 Mar 10;84(5):671-674.