Chlortetracycline, a fluorescent probe for pH of calcium stores in cells

Jose Jacob; Deepthi Chandran; Ranjana Sasidharan; Leena Kuruvila; U. K. Madhusudan; Nivedita L. Rao; Dibyajyoti Banerjee

Chlortetracycline, a fluorescent probe for pH of calcium stores in cells

Jose Jacob, Deepthi Chandran, Ranjana Sasidharan, Leena Kuruvila, U. K. Madhusudan, Nivedita L. Rao, Dibyajyoti Banerjee

Research output: Contribution to journal › Article › peer-review

3 Citations (Scopus)

Abstract

Endoplasmic reticulum is the major storage site for Ca²⁺ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H⁺ concentration, in addition to its established sensitivity to Ca²⁺. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca²⁺ with decrease in pH. Concentrations of H⁺ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H⁺ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.

Original language	English
Pages (from-to)	671-674
Number of pages	4
Journal	Current Science
Volume	84
Issue number	5
Publication status	Published - 10-03-2003
Externally published	Yes

All Science Journal Classification (ASJC) codes

Agricultural and Biological Sciences(all)
General

Cite this

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title = "Chlortetracycline, a fluorescent probe for pH of calcium stores in cells",

abstract = "Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.",

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AU - Jacob, Jose

AU - Chandran, Deepthi

AU - Sasidharan, Ranjana

AU - Kuruvila, Leena

AU - Madhusudan, U. K.

AU - Rao, Nivedita L.

AU - Banerjee, Dibyajyoti

PY - 2003/3/10

Y1 - 2003/3/10

N2 - Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.

AB - Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.

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Chlortetracycline, a fluorescent probe for pH of calcium stores in cells

Abstract

All Science Journal Classification (ASJC) codes

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