Endoplasmic reticulum is the major storage site for Ca2+ that is mobilized during activation of cell functions. Chlortetracycline (CTC) loaded into calcium stores of fresh, isolated human blood lymphocytes was found to be sensitive to changes in H+ concentration, in addition to its established sensitivity to Ca2+. Determination of pH of calcium stores was based on dissociation of fluorescent CTC·Ca2+ with decrease in pH. Concentrations of H+ at the calcium stores were calibrated to different levels by equilibrating it with different extracellular pH buffers using the H+ ionophore, nigericin. It was possible to determine the pH of two pools of calcium stores; they were 7.2 and 6.2.
|Number of pages||4|
|Publication status||Published - 10-03-2003|
All Science Journal Classification (ASJC) codes
- Agricultural and Biological Sciences(all)