Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma

F. Zölzer, O. Basu, P. U. Devi, S. P. Mohanty, C. Streffer

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Objectives: Proliferating cell nuclear antigen (PCNA) has often been used as a marker to aid assessment of tumour growth fraction. This paper addresses the question of whether it can be used as an S-phase marker, when the non-chromatin-bound form of the protein is removed by pepsin treatment.Materials and methods: Cytofluorometric measurements were carried out after immunofluorescence staining of PCNA and counterstaining of DNA. S-phase fraction was determined with the help of windows on PCNA versus DNA scattergrams, or mathematically from DNA histograms.Results: S-phase fractions obtained using the two methods correlated well, but did not always agree, exact discrepancies depending on the mathematical model used for histogram analysis.Conclusions: Determination of S-phase fractions with the help of PCNA immunofluorescence staining is possible, and probably more reliable than calculation of S-fractions from DNA histograms. It thus offers an alternative to assays involving BrdU labelling in vivo.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalCell Proliferation
Volume43
Issue number6
DOIs
Publication statusPublished - 01-12-2010

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Proliferating Cell Nuclear Antigen
Multiple Myeloma
S Phase
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Chromatin
Blood Cells
DNA
Fluorescent Antibody Technique
Staining and Labeling
Pepsin A
Bromodeoxyuridine
Theoretical Models
Growth
Neoplasms
Proteins
Therapeutics

All Science Journal Classification (ASJC) codes

  • Cell Biology

Cite this

Zölzer, F. ; Basu, O. ; Devi, P. U. ; Mohanty, S. P. ; Streffer, C. / Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma. In: Cell Proliferation. 2010 ; Vol. 43, No. 6. pp. 579-583.
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Chromatin-bound PCNA as S-phase marker in mononuclear blood cells of patients with acute lymphoblastic leukaemia or multiple myeloma. / Zölzer, F.; Basu, O.; Devi, P. U.; Mohanty, S. P.; Streffer, C.

In: Cell Proliferation, Vol. 43, No. 6, 01.12.2010, p. 579-583.

Research output: Contribution to journalArticle

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AU - Basu, O.

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AU - Mohanty, S. P.

AU - Streffer, C.

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