TY - JOUR
T1 - Circulation of Asian-I and Cosmopolitan genotypes of Dengue-2 virus in northeast India, 2016-2017
AU - Ujwal, S.
AU - Sabeena, S.
AU - Bhaskar, R.
AU - D'Souza, G.
AU - Santhosha, D.
AU - Auti, A.
AU - Kumar, R.
AU - Ramachandran, S.
AU - Hindol, Maity
AU - Aithal, A.
AU - Bhandari, Y.
AU - Jazeel, A.
AU - Arunkumar, G.
N1 - Funding Information:
The study was partially supported by CDC Cooperative Agreement (5U01GH001051) and ICMR grant File No. 5/8/7/15/2010/ECD-I). The authors sincerely acknowledge the help of the clinicians in Sonapur District Hospital, Assam and Sub-District Hospital, Longtarai, Manu, Tripura state and express their gratitude to the State health officials of Assam and Tripura for supporting the study.
Publisher Copyright:
© 2019 Wolters Kluwer Medknow Publications. All rights reserved.
Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2019/9
Y1 - 2019/9
N2 - Background & objectives: Dengue is a major public health problem in northeast India where the majority of the cases go unreported and undiagnosed. Even though all four dengue serotypes are reported, there is a dearth of information on genetic diversity. The present cross-sectional study was undertaken during 2016-17 to determine the genetic variance of dengue virus serotype 2 (DENV-2) based on the envelope (E) glycoprotein gene. Methods: The serum samples collected from the northeast parts of India, as a part of hospital-based acute febrile illness surveillance, were serotyped. Viral RNA was extracted from DENV-2 serum samples using QIAquick® RNA Extraction Kit. The E gene was amplified by conventional reverse-transcriptase polymerase chain reaction (RT-PCR) and the PCR products were sequenced. Results: The E glycoprotein gene of nine serum samples with high viral RNA concentration (Ct <25) was sequenced. The E gene sequences of eight DENV-2 strains from Assam and Meghalaya aligned with genotype IV (Cosmopolitan) and one strain from Tripura segregated with Asian-I genotype. Interpretation & conclusion: Ongoing laboratory-based surveillance is mandatory to understand the transmission dynamics of dengue in endemic countries. This study concluded that in northeast India, presently two distinct genotypes of DENV-2, namely genotype IVb (Cosmopolitan) and Asian-I genotype are in circulation.
AB - Background & objectives: Dengue is a major public health problem in northeast India where the majority of the cases go unreported and undiagnosed. Even though all four dengue serotypes are reported, there is a dearth of information on genetic diversity. The present cross-sectional study was undertaken during 2016-17 to determine the genetic variance of dengue virus serotype 2 (DENV-2) based on the envelope (E) glycoprotein gene. Methods: The serum samples collected from the northeast parts of India, as a part of hospital-based acute febrile illness surveillance, were serotyped. Viral RNA was extracted from DENV-2 serum samples using QIAquick® RNA Extraction Kit. The E gene was amplified by conventional reverse-transcriptase polymerase chain reaction (RT-PCR) and the PCR products were sequenced. Results: The E glycoprotein gene of nine serum samples with high viral RNA concentration (Ct <25) was sequenced. The E gene sequences of eight DENV-2 strains from Assam and Meghalaya aligned with genotype IV (Cosmopolitan) and one strain from Tripura segregated with Asian-I genotype. Interpretation & conclusion: Ongoing laboratory-based surveillance is mandatory to understand the transmission dynamics of dengue in endemic countries. This study concluded that in northeast India, presently two distinct genotypes of DENV-2, namely genotype IVb (Cosmopolitan) and Asian-I genotype are in circulation.
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U2 - 10.4103/0972-9062.289402
DO - 10.4103/0972-9062.289402
M3 - Article
C2 - 32655072
AN - SCOPUS:85087902440
SN - 0972-9062
VL - 56
SP - 231
EP - 236
JO - Journal of Vector Borne Diseases
JF - Journal of Vector Borne Diseases
IS - 3
ER -
